Time-resolved characterization of a pulsed discharge in a stationary bubble

In recent years, plasma generation in water has been proposed for the application of water treatment. The process efficiency is believed to be improved by the introduction of bubbles in the plasma active region. For further optimization, the initiating and developmental mechanisms of plasma inside bubbles need to be understood to a greater extent. In order to meet this necessity, we investigated pulsed electrical discharge inside a stationary bubble in water. This paper deals with the evolution of the discharge and of the bubble shape during discharge, investigated by electrical characterization and fast imaging. Only several microseconds after the application of the voltage pulse, plasma light is observed. Different phases are observed during plasma formation. The plasma is strongest at the bubble surface, causing the surrounding water to evaporate. This leads to both the formation of propagating streamers into the water and the expansion and collapse of the bubble. These observations show that plasma inside a bubble has the strongest activity at the bubble surface, making it attractive for water treatment

Interaction of hemojuvelin with neogenin results in iron accumulation in human embryonic kidney 293 cells

Type 2 hereditary hemochromatosis (HH) or juvenile hemochromatosis is an early onset, genetically heterogeneous, autosomal recessive disorder of iron overload. Type 2A HH is caused by mutations in the recently cloned hemojuvelin gene (HJV; also called HFE2) (Papanikolaou, G., Samuels, M. E., Ludwig, E. H., MacDonald, M. L., Franchini, P. L., Dube, M. P., Andres, L., MacFarlane, J., Sakellaropoulos, N., Politou, M., Nemeth, E., Thompson, J., Risler, J. K., Zaborowska, C., Babakaiff, R., Radomski, C. C., Pape, T. D., Davidas, O., Christakis, J., Brissot, P., Lockitch, G., Ganz, T., Hayden, M. R., and Goldberg, Y. P. (2004) Nat. Genet. 36, 77–82), whereas Type 2B HH is caused by mutations in hepcidin. HJV is highly expressed in both skeletal muscle and liver. Mutations in HJV are implicated in the majority of diagnosed juvenile hemochromatosis patients. In this study, we stably transfected HJV cDNA into human embryonic kidney 293 cells and characterized the processing of HJV and its effect on iron homeostasis. Our results indicate that HJV is a glycosylphosphatidylinositol-linked protein and undergoes a partial autocatalytic cleavage during its intracellular processing. HJV co-immunoprecipitated with neogenin, a receptor involved in a variety of cellular signaling processes. It did not interact with the closely related receptor DCC (deleted in Colon Cancer). In addition, the HJV G320V mutant implicated in Type 2A HH did not co-immunoprecipitate with neogenin. Immunoblot analysis of ferritin levels and transferrin-55Fe accumulation studies indicated that the HJV-induced increase in intracellular iron levels in human embryonic kidney 293 cells is dependent on the presence of neogenin in the cells, thus linking these two proteins to intracellular iron homeostasis

Nucleon-nucleon cross sections in neutron-rich matter and isospin transport in heavy-ion reactions at intermediate energies

Nucleon-nucleon (NN) cross sections are evaluated in neutron-rich matter using a scaling model according to nucleon effective masses. It is found that the in-medium NN cross sections are not only reduced but also have a different isospin dependence compared with the free-space ones. Because of the neutron-proton effective mass splitting the difference between nn and pp scattering cross sections increases with the increasing isospin asymmetry of the medium. Within the transport model IBUU04, the in-medium NN cross sections are found to influence significantly the isospin transport in heavy-ion reactions. With the in-medium NN cross sections, a symmetry energy of $E_{sym}(\rho)\approx 31.6(\rho /\rho_{0})^{0.69}$ was found most acceptable compared with both the MSU isospin diffusion data and the presently acceptable neutron-skin thickness in $^{208}$Pb. The isospin dependent part $K_{asy}(\rho _{0})$ of isobaric nuclear incompressibility was further narrowed down to $-500\pm 50$ MeV. The possibility of determining simultaneously the in-medium NN cross sections and the symmetry energy was also studied. The proton transverse flow, or even better the combined transverse flow of neutrons and protons, can be used as a probe of the in-medium NN cross sections without much hindrance from the uncertainties of the symmetry energy.Comment: 32 pages including 14 figures. Submitted to Phys. Rev.

Determination of the stiffness of the nuclear symmetry energy from isospin diffusion

With an isospin- and momentum-dependent transport model, we find that the degree of isospin diffusion in heavy ion collisions at intermediate energies is affected by both the stiffness of the nuclear symmetry energy and the momentum dependence of the nucleon potential. Using a momentum dependence derived from the Gogny effective interaction, recent experimental data from NSCL/MSU on isospin diffusion are shown to be consistent with a nuclear symmetry energy given by $E_{\text{sym}}(\rho)\approx 31.6(\rho /\rho_{0})^{1.05}$ at subnormal densities. This leads to a significantly constrained value of about -550 MeV for the isospin-dependent part of the isobaric incompressibility of isospin asymmetric nuclear matter.Comment: 4 pages, 4 figures, 1 table, revised version, to appear in PR

Random Surfing Without Teleportation

In the standard Random Surfer Model, the teleportation matrix is necessary to ensure that the final PageRank vector is well-defined. The introduction of this matrix, however, results in serious problems and imposes fundamental limitations to the quality of the ranking vectors. In this work, building on the recently proposed NCDawareRank framework, we exploit the decomposition of the underlying space into blocks, and we derive easy to check necessary and sufficient conditions for random surfing without teleportation.Comment: 13 pages. Published in the Volume: "Algorithms, Probability, Networks and Games, Springer-Verlag, 2015". (The updated version corrects small typos/errors

Differential isospin-fractionation in dilute asymmetric nuclear matter

The differential isospin-fractionation (IsoF) during the liquid-gas phase transition in dilute asymmetric nuclear matter is studied as a function of nucleon momentum. Within a self-consistent thermal model it is shown that the neutron/proton ratio of the gas phase becomes {\it smaller} than that of the liquid phase for energetic nucleons, although the gas phase is overall more neutron-rich. Clear indications of the differential IsoF consistent with the thermal model predictions are demonstrated within a transport model for heavy-ion reactions. Future comparisons with experimental data will allow us to extract critical information about the momentum dependence of the isovector strong interaction.Comment: Rapid Communication, Phys. Rev. C (2007) in pres

Lessons from LIMK1 enzymology and their impact on inhibitor design

LIM domain kinase 1 (LIMK1) is a key regulator of actin dynamics. It is thereby a potential therapeutic target for the prevention of fragile X syndrome and amyotrophic lateral sclerosis. Herein, we use X-ray crystallography and activity assays to describe how LIMK1 accomplishes substrate specificity, to suggest a unique ‘rock-and-poke’ mechanism of catalysis and to explore the regulation of the kinase by activation loop phosphorylation. Based on these findings, a differential scanning fluorimetry assay and a RapidFire mass spectrometry activity assay were established, leading to the discovery and confirmation of a set of small-molecule LIMK1 inhibitors. Interestingly, several of the inhibitors were inactive towards the closely related isoform LIMK2. Finally, crystal structures of the LIMK1 kinase domain in complex with inhibitors (PF-477736 and staurosporine, respectively) are presented, providing insights into LIMK1 plasticity upon inhibitor binding

Nuclear symmetry energy and its density slope at normal density extracted from global nucleon optical potentials

Based on the Hugenholtz-Van Hove theorem, it is shown that both the symmetry energy E$_{sym}(\rho)$ and its density slope $L(\rho)$ at normal density $\rho_0$ are completely determined by the global nucleon optical potentials that can be extracted directly from nucleon-nucleus scatterings, (p,n) charge exchange reactions and single-particle energy levels of bound states. Adopting a value of $m^*/m=0.7$ for the nucleon effective k-mass in symmetric nuclear matter at $\rho_0$ and averaging all phenomenological isovector nucleon potentials constrained by world data available in the literature since 1969, the best estimates of $E_{sym}(\rho_0)=31.3$ MeV and $L(\rho_0)=52.7$ MeV are simultaneously obtained. Uncertainties involved in the estimates are discussed.Comment: 4 pages including 2 figure

Regional and temporal changes in AIDS in Europe before HAART

In a prospective observational study 4485 patients from 46 clinical centres in 17 European countries were followed between April 1994 and November 1996. Information on AIDS-defining events (ADEs) were collected together with basic demographic data, treatment history and laboratory results. The centres were divided into four geographical regions (north, central, south-west and south-east) so that it was possible to identify any existing regional differences in ADEs. The regional differences that we observed included a higher risk of all forms of Mycobacterium tuberculosis infections (Tb) and wasting disease in the south-west and an increased risk of infections with the Mycobacterium avium complex (MAC) in the north. In Cox multivariable analyses, where north was used as the reference group, we observed hazard ratios of 6.87, 7.77, 2.29 and 0.16 (P < 0.05 in all cases) for pulmonary Tb, extrapulmonary Tb, wasting disease and MAC respectively in the south-west. Pneumocystis carinii pneumonia (PCP) was less commonly diagnosed in the central region (RH = 0.51, 95% CI 0.32-0.79, P = 0.003) and most common in the south-east (RH = 1.04, 95% CI 0.71-1.51, P = 0.85). Comparisons with a similar 'AIDS in Europe' study that concentrated on the early phase of the epidemic reveal that most of the regional differences that were observed in the 1980s still persist in the mid-1990s

Current measurement by real-time counting of single electrons

The fact that electrical current is carried by individual charges has been known for over 100 years, yet this discreteness has not been directly observed so far. Almost all current measurements involve measuring the voltage drop across a resistor, using Ohm's law, in which the discrete nature of charge does not come into play. However, by sending a direct current through a microelectronic circuit with a chain of islands connected by small tunnel junctions, the individual electrons can be observed one by one. The quantum mechanical tunnelling of single charges in this one-dimensional array is time correlated, and consequently the detected signal has the average frequency f=I/e, where I is the current and e is the electron charge. Here we report a direct observation of these time-correlated single-electron tunnelling oscillations, and show electron counting in the range 5 fA-1 pA. This represents a fundamentally new way to measure extremely small currents, without offset or drift. Moreover, our current measurement, which is based on electron counting, is self-calibrated, as the measured frequency is related to the current only by a natural constant.Comment: 9 pages, 4 figures; v2: minor revisions, 2 refs added, words added to title, typos correcte