Health Disparities in Cardiovascular Disease and High Blood Pressure Among Adults in Rural Underserved Communities

Purpose: This study examined the factors contributing to health disparities in cardiovascular disease (CVD) and high blood pressure (HBP) among adults in three rural underserved communities in southeast Georgia. Socioeconomic status as well as geographic location plays a significant role in one’s quality of health outcomes. Methods: Individuals in three counties in southern Georgia participated in the study. The study was motivated by review of retrospective data from the 2008 Georgia Cardiovascular Health Initiative (CVHI) database to explain the factors contributing to the incidence of health disparities. A survey questionnaire was administered by telephone to adult members of households to determine the incidence of health disparities in CVD and HBP among rural African American and White adult populations. Six hundred respondents participated in the survey but four hundred completed surveys were used in the study, yielding a 67% response rate. Data were analyzed using applied multivariate logistic analysis. Findings: Findings indicated that older men and male residents in Counties A and B regardless of racial background were significantly more likely to be diagnosed with both HBP and CVD. College educated women were significantly less likely to have HBP. Findings also revealed that married men were significantly less likely to have CVD. Uncontrolled elevated cholesterol levels contributed to the incidence of chronic HBP and CVD. Conclusions: The findings add to the current knowledge of research and to the understanding of the critical elements in reducing health disparities among populations in rural underserved communities

Bioanalysis of aminoglycosides using high-performance liquid chromatography

Aminoglycosides are broad-spectrum antibiotics used in the treatment of gram-negative bacterial infections. Due to their nephrotoxic and ototoxic potential (narrow therapeutic index), the use of aminoglycoside for clinical indications requires monitoring. The objective of this review was to identify relevant literature reporting liquid chromatographic methods for the bioanalysis of aminoglycosides in both preclinical and clinical settings/experiments. Data on liquid chromatographic methods were collected from articles in an online academic database (PubMed, Science Direct, Scopus, and Google Scholar). All 71 articles published from 1977 to 2020 were included in the review. Reversed-phase liquid chromatography was the most used method for the bioanalysis of aminoglycosides. Fluorescence or ultraviolet detection methods were mostly used from 1977 to 2002 (51 articles), while mass spectrometry was predominantly used as a detector from 2003 to 2020 (15 articles). Sixty-seven articles reported calibration ranges, which varied significantly for the various drugs assayed: some in the range of 0.1-0.5 ng/mL and others 1250-200000 ng/mL. Also, 61 articles reported R2 values (0.964-1.0) for almost all analytes under consideration. Sixty-three articles reported percent recoveries mostly between 61.0 % to 114.0 %, with only two articles reporting recoveries of 4.9 % and 36 %. Out of the 71 reviewed articles, 56 reported intermediate precision values ranging between 0.331 % to 19.76 %, which is within the acceptable limit of 20 %. This review will serve as a guide for research and/or routine clinical monitoring of aminoglycosides in biological matrices

Impacto de las actividades de construcción de puertos en la estructura, composición y diversidad de la población de peces: estudio de caso de Tema, Ghana

Ports offer economic progress to many coastal developing countries. In view of the economic importance, the rising demand for maritime benefits requires the expansion of existing ports. However, marine ecosystems may become vulnerable to negative impact from the construction of these maritime structures. Therefore, the aim of the study was to establish the impact of Tema port expansion on the diversity and population structure of fish species. Data were collected quarterly from June 2018 to November 2021 through trawling fishing activities off the coast of Greater Accra, Ghana, and subsequently analysed using Primer 6 software. Sampling locations along the coast of Greater Accra included Vernon Bank (VNB), Disposal area (DSA) and Offshore Sakumono (OSK). The increased and reduced number of species at VNB and DSA, respectively, could be alluded to dredging and disposal of dredged spoils. Diversity indices at OSK site was higher than those observed in disturbed areas due to the absence of impact in this control area. Thus, it is recommended to conduct further studies on the assemblage of invasive species that migrated to the VNB during dredging activities, since these species can have severe economic impact on fishing activities of artisanal fishermen, especially at the local level.Los puertos ofrecen progreso económico a muchos países costeros en desarrollo. En vista de la importancia económica, la creciente demanda de beneficios marítimos requiere la ampliación de los puertos existentes. Sin embargo, los ecosistemas marinos pueden volverse vulnerables al impacto negativo de la construcción de estas estructuras marítimas. Por lo tanto, el objetivo del estudio fue establecer el impacto de la expansión del puerto de Tema sobre la diversidad y estructura de la población de peces. Los datos se recopilaron trimestralmente desde junio de 2018 hasta noviembre de 2021 mediante actividades de pesca de arrastre fren-te a la costa del Gran Accra, Ghana. Los mismos se analizaron utilizando el software Primer 6. Los lugares de muestreo a lo largo de la costa del Gran Accra incluyeron Vernon Bank (VNB), Disposal area (DSA) y Offshore Sakumono (OSK). El mayor y menor número de especies en VNB y DSA, respectivamente, podría deberse al dragado y disposición de los escombros removidos. Los índices de diversidad en el sitio OSK fueron más altos que los observados en áreas perturbadas debido a la ausencia de impacto en esta área de control. Por lo tanto, se recomienda realizar más estudios sobre las asociaciones de especies invasoras que migraron al VNB durante las actividades de dragado, ya que estas especies pueden tener severos impactos económicos en la actividad pesquera de los pescadores artesanales, especialmente a nivel local

Elaboration of Additional Modules on Climate Smart Agriculture and Climate Information System for Staff, Students, and other Stakeholders in Universities in Africa

The Accelerating Impacts of CGIAR Climate Research for Africa project (AICCRA) working through CCAFS, intends to make CGIAR-led cutting-edge science practices/technologies/tools available throughout Africa; especially in Sub-regions extremely vulnerable to climate change. The Regional Universities Forum for Capacity Building in Agriculture (RUFORUM), a network of 150 universities in 38 countries spanning the whole African continent is a partner in the AICCRA project. RUFORUM’s contribution in the AICCRA project is focused on mobilising African universities, create awareness and enhance the use of Climate Smart Agriculture (CSA) and Climate information services (CIS) knowledge and products developed by the CGIAR Centres and other research institutions engaged in CSA and CIS. Enhancing the use of CSA and CIS involves capacity, knowledge and technology audits at national and institutional level, mobilise CGIAR and other research centres to provide CSA and CIS knowledge, technology and skills and training of faculty to deploy the CSA and CIS products in training, research and outreach. Knowledge transfer and capacity building activities therefore form the central part of RUFORUM’s participation in the AICCRA project

Why All the Hype about ChatGPT? Academics’ Views of a Chat-based Conversational Learning Strategy at an Open Distance e-Learning Institution

The launch of ChatGPT has been revolutionary. This AI chatbot can produce conversations which are indistinguishable from that of humans. This exploratory qualitative study is foregrounded in a constructivist-interpretative perspective. The principal objective of this paper is to explore the views of academics on ChatGPT as an AI-based learning strategy at an open distance e-learning (ODeL) institution of higher education. Thirteen academics, who were enrolled as study participants, posted their views of ChatGPT as an AI-based learning strategy on a Teams chat at an institution of higher learning. The results support a few research studies on ChatGPT. The academics recognized the benefits and risks of using ChatGPT for teaching and learning. Further investigations are recommended to explore similar studies in higher education spaces and specifications

Effect of organic fertilizers on physical and chemical quality of sugar loaf pineapple (Ananas comosus L) grown in two ecological sites in Ghana

Pineapple has a large demand for plant nutrients and for this reason; fertilization is almost mandatory where the fruit is destined for sale. As consumer demand for organic food grows, organic production and certification is seen as a valuable alternative for smallholder farmers in developing countries. This study sought to investigate the effects of different organic fertilizers on the physical and chemical quality of pineapple (Ananas comosus L.) cultivated in two different ecological zones in Ghana. Organic fertilizers treatments were POME (Palm oil meal effluent), Phos-K (PH), Yara (Y) and combination of Phos-K and POME (PH+P) and Yara + POME (Y+P) in the ratio of 1:1. Soil without fertilizer was control. A farmer field demonstration and an on-station experimental trial were conducted parallel at Nsakyi and at the Biotechnology and Nuclear Agriculture Research Institute (BNARI) research farm, respectively. The experiment was laid out in the Randomised Completely Block Randomized Design (RCBD) with five fertilizer treatments and four replications. Sugar loaf pineapples were harvested at 17 months upon maturity, washed, peeled, and juice extracted for analyzing vitamin C, Total Soluble Solids, pH, Titratable acidity, juice yield and colour. Fruits were weighed with Sartorius scale and percentage weight loss estimated over 15 days. The weight loss of pineapples treated with POME (25.56 ± 0.62 %) from BNARI farm was significantly (p<0.05) higher than all the other pineapple treaments. pH of all pineapple samples significantly (P<0.05) differed for all the different fertilizer treatments for both farms. Phos-K significantly (p<0.05) enhanced the vitamin C content of pineapples from both ecological sites Lightness (L*) and yellowness (b*) for juice of pineapple treated with Phos-K from BNARI farm was significantly (p<0.05) higher, than samples from Nsakyi farms indicating desirable visual appeal for sugar loaf pineapples. Organic fertilizers significantly (p<0.05) influenced the pH, vitamin C and juice yield of pineapples grown in the two different ecological zones. Irrespective of the ecological differences in Nsakyi and BNARI farm sites, the application of Phos-K to pineapple significantly (p<0.05) increased the juice yield which is a desirable quality of pineapple. Thus the application of Phos-K organic fertilizer, better improved the physical and chemical quality attributes of pineapple. However, there is the need to conduct further work on application rate and utilization efficiency of organic fertilizers that will produce maximum pineapple quality and yield.Keywords: Organic fertilizers, Juice, weight los

Evaluating the content validity of two versions of an instrument used in measuring pediatric pain knowledge and attitudes in the Ghanaian context

In this article, we compared the content validity of two instruments used in measuring pediatric pain knowledge and attitudes. This was considered necessary due to the universal differences in culture, semantics and healthcare resources in different parts of the globe. Thirteen (13) pediatric experts in Ghana assessed the content validity of two instruments: the 42-item Pediatric Nurses' Knowledge and Attitudes Survey Regarding Pain (PNKAS) and the 41-item Pediatric Healthcare Providers' Knowledge and Attitudes Survey Regarding Pain (PHPKASRP). The relevance and clarity of each item on these instruments were rated on a four-point likert scaled options from 1 (not relevant/ not clear) to 4 (very relevant/ very clear). The item-level content validity index (I-CVI) was calculated by dividing the number of experts who rated an item with 3 or 4 by the total number of experts. The average scale-level content validity index (S-CVI/Ave) was also estimated by summing up the I-CVIs of all items and dividing them by the total number of items. The I-CVIs on the PNKAS ranged from 0.62 to 1.00 for the relevance component and 0.69 to 1.00 for the clarity component. The I-CVIs on the PHPKASRP ranged from 0.62 to 1.00 for both the relevance and clarity components. The S-CVI/Ave were 0.87 and 0.89 for the relevance and clarity aspects on the PNKAS respectively. The S-CVI/Ave for the PHPKASRP instrument were 0.86 and 0.89 for the relevance and clarity aspects correspondingly. At the end of the validation process, 5 items were revised on both instruments whilst 37 and 36 items were maintained on the PNKAS and PHPKASRP instruments respectively. The PNKAS and PHPKASRP have an acceptable level of content validity in the Ghanaian context and recommended for educational and research purposes. Other forms of validity and reliability of these instruments should also be examined in future studies.</p

ANTIOXIDANT AND ANTI-PROLIFERATIVE EFFECTS OF AN ETHYL ACETATE FRACTION OF THE HYDRO-ETHANOLIC EXTRACT OF SYNEDRELLA NODIFLORA (L) GAERTN

Objective: Synedrella nodiflora is traditionally used in the treatment of several ailments. Pharmacologically, this plant has anticonvulsant, sedative, anti-nociceptive and anti-proliferative effects. This study further investigated S. nodiflora for its antioxidant and in vitro inhibition of cancerous cell lines. Methods: Phytochemical assays, and the DPPH radical scavenging method were employed in preliminary screening for antioxidant activities of the crude hydro-ethanolic extract (SNE) and resulting fractions. The potent ethyl acetate fraction (EAF), was further investigated for total phenol and flavonoid contents, reducing power, lipid peroxidation potential, and cytotoxic effects on human breast cancer (MCF-7), leukemic (Jurkat), and normal liver (Chang’s liver) cell lines. Results: The extract contained phenols, flavonoids, tannins, glycosides, sterols, terpenoids, and alkaloids. It scavenged for DPPH with an IC50 of 114 µg/ml, whereas that of EAF was 8.9 µg/ml. EAF prevented peroxidation of egg lecithin at an IC50 of 24.01±0.08 µg/ml. These IC50s are four and three times lower than the reference standards. EAF produced anti-proliferative effects against MCF-7, and Jurkat cell lines with IC50s of 205.2 and 170.9 µg/ml, respectively. EAF had a high IC50 of 252.2 µg/ml against Chang’s liver cells. At 0.1 mg/ml EAF had similar total flavonoid content to SNE, but a significantly higher total phenol content. Conclusion: The ethyl acetate fraction of S. nodiflora, exhibited the most potent antioxidant activity. It inhibited the proliferation of breast and leukemic cancer cell lines, whiles having weak cytotoxic effect on normal liver cells. These can be explored for further drug development

Adapted motivational interviewing to improve the uptake of treatment for glaucoma in Nigeria: study protocol for a randomized controlled trial.

BACKGROUND: Glaucoma is a chronic eye disease associated with irreversible visual loss. In Africa, glaucoma patients often present late, with very advanced disease. One-off procedures, such as laser or surgery, are recommended in Africa because of lack of or poor adherence to medical treatment. However, acceptance of surgery is usually extremely low. To prevent blindness, adherence to treatment needs to improve, using acceptable, replicable and cost-effective interventions. After reviewing the literature and interviewing patients in Bauchi (Nigeria) motivational interviewing (MI) was selected as the intervention for this trial, with adaptation for glaucoma (MIG). MI is designed to strengthen personal motivation for, and commitment to a specific goal by eliciting and exploring a person's reasons for change within an atmosphere of acceptance and compassion. The aim of this study is to assess whether MIG increases the uptake of laser or surgery amongst glaucoma patients where this is the recommended treatment. The hypothesis is that MIG increases the uptake of treatment. This will be the first trial of MI in Africa. METHODS: This is a hospital based, single centre, randomized controlled trial of MIG plus an information sheet on glaucoma and its treatment (the latter being "standard care") compared with standard care alone for glaucoma patients where the treatment recommended is surgery or laser.Those eligible for the trial are adults aged 17 years and above who live within 200 km of Bauchi with advanced glaucoma where the examining ophthalmologist recommends surgery or laser. After obtaining written informed consent, participants will be randomly allocated to MIG plus standard care, or standard care alone. Motivational interviewing will be delivered in Hausa or English by one of two MIG trained personnel. One hundred and fifty participants will be recruited to each arm. The primary outcome is the proportion of participants undergoing laser or surgery within two months of the date given to re attend for the procedure. MIG quality will be assessed using the validated MI treatment integrity scale. DISCUSSION: Motivational interviewing may be an important tool to increase the acceptance of treatment for glaucoma. The approach is potentially scalable and may be useful for other chronic conditions in Africa. TRIAL REGISTRATION: ISRCTN79330571 (Controlled-Trials.com)