Examination of Faecal Caliform Presence in Groundwater in Ghana (A Case Study in Ketu South Municipality)

Samples were taken from wide-dam, open wells and compared with piped city water. Well water from  both districts showed widespread contamination with faecal bacteria. This study revealed high Faecal and Total coliforms in all the samples with varying numbers. The high coliforms content of the eight hand-dug wells poses a health risk and renders the water unsuitable for human consumption. The presence of pathogenic organisms in the water could be attributed to pit latrine in the vicinity that extent their influence on these water qualities, since they are sited close to them. © JASE

Evaluation of chemical properties in wells in Ghana (A case study in Ho Municipality)

The most important inorganic contaminant is nitrate ion, NO3-.Excess nitrate ion in drinking water is a potential health hazard: risk of methemoglobinemia (or “blue-baby” syndrome). Sources of nitrate in groundwater: nitrogen fertilizers, atmospheric deposition, human sewage deposited in septic systems. The results of the study show that the values of parameters of the water samples such as, alkalinity, phosphate and nitrate etc are higher than WHO standards. None of the samples had nil value of nitrates and nitrites. The levels of nitrogen ammonium were less than WHO limits of 1.5 mg/L. All the samples had the phosphate concentration higher than 0.01 mg/L (WHO) Guideline value. The mean values also exceeded the limits. It was recommended that, the focus of any programme designed to deliver safe drinking water should therefore be the effective management and operation of water sources, treatment plants and distribution systems whether piped or manual. This will demand action by water suppliers, environmental protection agencies and health bodies.

A simple method for the extension of shelf life of cultures of Phytophthora species causing black pod disease of cacao (Theobroma cacao L.)

Black pod disease of cacao caused by Phytophthora palmivora and Phytophthora megakarya in Ghana take heavy toll of cacao production in the field. Intensive research has been carried out worldwide on these pathogens. However, viability of the cultures during prolonged storage has remained a major challenge in the research. This paper reports findings of assessment of six storage media viz sterilised distilled water (SDW), sterilised and unsterilized soil suspension (SSS and USS), vegetable 8 juice broth (V8JB), Oat Meal Agar slant under mineral oil (at 4°C) and empty tube. Viability of the cultures was assessed on V8JA and in tetrazolium chloride test. Ability of zoospores of the cultures to infect cacao leaf discs was used to assess growth vigour and pathogenicity. Phytophthora cultures stored in SDW (26 ± 2°C; alternating day light and night) were preserved for 60 days (5 years). Both P. palmivora and P. megakarya performed better on SDW and SSS than on USS due to removal of staling substances in the soil medium by the sterilisation. Vigour of growth and pathogenicity of the stored cultures required re-inoculation of host tissue (cacao pod) in order to maintain potency to continually infect host

Carbon Footprint of the Large Scale Gold Mining Industry of Ghana

Mining has been an important economic activity accounting for a substantial part of foreign exchange and government revenue. The gold mining sector has undergone considerable expansion due to liberalization with more foreign mining companies investing in the sector in particular large-scale open pit gold mining. Resultant expansion in mining has led to heightened environmental concerns and significant challenges due to key activities used in the mining operations hence contributing to greenhouse gas emissions. Investigations were conducted to assess the sources and key activities in large scale gold open pit mining operations in Ghana giving rise to greenhouse gas generation. The study adopted the Intergovernmental Panel on Climate Change (IPCC) guideline for estimating greenhouse gas inventory. The operational boundary as against the organizational boundary was considered for the study. The operational boundary was drawn around the physical mining site. Within this boundary, the emissions were quantified and reported under direct emission due to company activities (Scope 1) and indirect emissions from secondary use or activity (Scope 2). The study found that activities due to land use, blasting, fuel use to power mobile equipment and stationary combustion sources, electricity use and waste management were the contributing greenhouse gas emission sources in a large scale gold mining operation with electricity use and fuel used in transportation accounting for 92.46% of the total emissions. Average contribution of the large scale gold mining industry in Ghana to the total national greenhouse gas emission inventory for the country was established to be 11.08%

Connecting DNA Origami Structures Using the Biotin-Streptavidin Specific Binding

This work made use of the strong interaction between biotin and streptavidin to connect designed DNA origami structures. The caDNAno software was used to design a 6 layer 3D origami cross-like structure. Selected DNA strands at the engineered attachment sites on the DNA origami structure were biotinylated. After folding of the origami structures, the biotinylated strands stick out of the attachment sites. Purified samples of origami structures were then mixed with streptavidin and the mixture purified. After characterization, we see that attachment only occurs at the biotinylated sites. Agarose gel electrophoresis, UV-vis spectroscopy and TEM were used to characterize the structure.Key words: DNA Origami, Interaction, Biotin-Streptavidin, Nanomaterials, TEMAbbreviation: EDTA, Ethylenediaminetetraacetic acid; TEM, Transmission electron microscop

Gastroenterology practice in the COVID-19 era: Ghana Association for the Study of Liver and Digestive Diseases (GASLIDD) position statement

The COVID-19 pandemic has impacted healthcare negatively across the globe. The practice of gastroenterology has been affected especially gastrointestinal (GI) endoscopy which is considered high risk for transmission of the virus. As a community of practitioners there is the need to share information and make evidence-based statements to guide GI practice in Ghana. This GASLIDD position statement based on the growing and rapidly evolving body of knowledge is to provide up to date information on the COVID-19 disease and guidance for the practice of gastroenterology in Ghana and beyond. It is to help the GI community of practice to maintain the highest level of health delivery and safety for our patients, staff, community and GI practitioners

Diaspora entrepreneurs' push and pull institutional factors for investing in Africa: insights from African returnees from the United Kingdom

Applying the institution-based views, this article conceptualises how diaspora entrepreneurs take stimuli from the push and pull institutional factors to develop business enterprises in their countries of origin. Using cases of African diaspora entrepreneurs in the UK and the grounded theory methodological approach, our conceptualised model demonstrates that the diasporas use the new knowledge, skills and wealth they have gained in the UK in tandem with support from trusted family, kinship and business ties at home to develop enterprises. It further demonstrates that diaspora entrepreneurs foster resilience to withstand weak formal institutions in their countries of origin and the discriminatory obstacles in the UK. We also found that institutional barriers which served as push factors that encouraged or forced migrants to leave their home countries to seek greener pastures abroad may later become pull factors that enable them to engage in diaspora entrepreneurship which is often characterised by paradoxes. Particularly, the informal institutions that constrain foreign investors can become assets for African diaspora entrepreneurs and help them set up new businesses and exploit market opportunities in Africa. The implications of the study for diaspora entrepreneurship literature are outlined

Diversity and proliferation of metallo-β-lactamases : a clarion call for clinically effective metallo-β-lactamase inhibitors

The worldwide proliferation of life-threatening metallo-β-lactamase (MBLs)-producing Gram-negative bacteria is a serious concern to public health. MBLs are compromising the therapeutic efficacies of β-lactams, particularly carbapenems, which are last-resort antibiotics indicated for various multidrug-resistant bacterial infections. Inhibiting enzymes mediating antibiotic resistance in bacteria is one of the major promising means in overcoming bacterial resistance. Compounds having potential MBLs-inhibitory activity have been reported, but none are currently under clinical trials. The need for developing safe and efficient MBL inhibitors (MBLIs) is obvious, particularly with the continuous spread of MBLs worldwide. In this review, the emergence and escalation of MBLs in Gram-negative bacteria are dicussed. The relationship between different class B β-lactamases identified up to 2017 are represented by a phylogenetic tree and summarized. On the other hand, approved and/or clinical-phase serine β-lactamase inhibitors are recapitulated to reflect the successful advances made in developing class A β-lactamase inhibitors. Reported MBLIs, their inhibitory properties and purported mode of inhibition are herein delineated. Insights into MBLs' structural variations and the challenges involved in developing potent MBLIs are also elucidated and discussed. Currently, natural products and MBL-resistant β-lactam analogues are the most promising agents that can become clinically efficient MBLIs. A deeper comprehension of the mechanism of action and activity spectrum of the various MBLs and their inhibitors will serve as a bedrock for further investigations that can result in clinically useful MBLIs to curb this global menace.http://aem.asm.org2019-03-01hj2018Medical Microbiolog

Individual Readiness for Change in the Context of Enterprise Resource Planning Systems Implementation

The present study takes a bottom-up approach and investigates the organizational implications of ERP systems implementation in organizations. We adopt a likely point of view of employees and study the ERP integration process along 3 dimensions: people, processes, and information. In this manner we discover the ERP-specific sources of resistance that could affect negatively the deployment of the software. Then, we argue that a general set of beliefs shapes employees readiness to change to ERP use and provides the foundation for resistance or for adoptive behavior. We define the concept of readiness for change in the context of ERP and introduce a readiness for change assessment approach. Then, we test empirically the study hypotheses upon which the research model was build. The results obtained offer insights into factors that can improve the effectiveness of ERP implementation strategies and underline the importance of change management for the success of such projects