On Equivalence of Critical Collapse of Non-Abelian Fields

We continue our study of the gravitational collapse of spherically symmetric skyrmions. For certain families of initial data, we find the discretely self-similar Type II critical transition characterized by the mass scaling exponent $\gamma \approx 0.20$ and the echoing period $\Delta \approx 0.74$. We argue that the coincidence of these critical exponents with those found previously in the Einstein-Yang-Mills model is not accidental but, in fact, the two models belong to the same universality class.Comment: 7 pages, REVTex, 2 figures included, accepted for publication in Physical Review

Pre-Existing Adenovirus Immunity Modifies a Complex Mixed Th1 and Th2 Cytokine Response to an Ad5/HIV-1 Vaccine Candidate in Humans

The results of the recent Step Study highlight a need to clarify the effects of pre-existing natural immunity to a vaccine vector on vaccine-induced T-cell responses. To investigate this interaction, we examined the relationship between pre-existing Ad5 immunity and T-cell cytokine response profiles in healthy, HIV-uninfected recipients of MRKAd5 HIV-1 gag vaccine (HVTN 050, ClinicalTrials.gov #NCT00849732). Participants were grouped by baseline Ad5 neutralizing antibody titer as either Ad5-seronegative (titer ≤18; n = 36) or Ad5-seropositive (titer >200; n = 34). Samples from vaccine recipients were analyzed for immune responses to either HIV-1 Gag peptide pools or Ad5 empty vector using an ex vivo assay that measures thirty cytokines in the absence of long-term culture. The overall profiles of cytokine responses to Gag and Ad5 had similar combinations of induced Th1- and Th2-type cytokines, including IFN-γ, IL-2, TNF-α, IP-10, IL-13, and IL-10, although the Ad5-specific responses were uniformly higher than the Gag-specific responses (p<0.0001 for 9 out of 11 significantly expressed analytes). At the peak response time point, PBMC from Ad5-seronegative vaccinees secreted significantly more IP-10 in response to Gag (p = 0.008), and significantly more IP-10 (p = 0.0009), IL-2 (p = 0.006) and IL-10 (p = 0.05) in response to Ad5 empty vector than PBMC from Ad5-seropositive vaccinees. Additionally, similar responses to the Ad5 vector prior to vaccination were observed in almost all subjects, regardless of Ad5 neutralizing antibody status, and the levels of secreted IFN-γ, IL-10, IL-1Ra and GM-CSF were blunted following vaccination. The cytokine response profile of Gag-specific T cells mirrored the Ad5-specific response present in all subjects before vaccination, and included a number of Th1- and Th2-associated cytokines not routinely assessed in current vaccine trials, such as IP-10, IL-10, IL-13, and GM-CSF. Together, these results suggest that vector-specific humoral responses may reduce vaccine-induced T-cell responses by previously undetected mechanisms

A Cell Permeable Peptide Inhibitor of NFAT Inhibits Macrophage Cytokine Expression and Ameliorates Experimental Colitis

Nuclear factor of activated T cells (NFAT) plays a critical role in the development and function of immune and non-immune cells. Although NFAT is a central transcriptional regulator of T cell cytokines, its role in macrophage specific gene expression is less defined. Previous work from our group demonstrated that NFAT regulates Il12b gene expression in macrophages. Here, we further investigate NFAT function in murine macrophages and determined the effects of a cell permeable NFAT inhibitor peptide 11R-VIVIT on experimental colitis in mice. Treatment of bone marrow derived macrophages (BMDMs) with tacrolimus or 11R-VIVIT significantly inhibited LPS and LPS plus IFN-γ induced IL-12 p40 mRNA and protein expression. IL-12 p70 and IL-23 secretion were also decreased. NFAT nuclear translocation and binding to the IL-12 p40 promoter was reduced by NFAT inhibition. Experiments in BMDMs from IL-10 deficient (Il10−/−) mice demonstrate that inhibition of IL-12 expression by 11R-VIVIT was independent of IL-10 expression. To test its therapeutic potential, 11R-VIVIT was administered systemically to Il10−/− mice with piroxicam-induced colitis. 11R-VIVIT treated mice demonstrated significant improvement in colitis compared to mice treated with an inactive peptide. Moreover, decreased spontaneous secretion of IL-12 p40 and TNF in supernatants from colon explant cultures was demonstrated. In summary, NFAT, widely recognized for its role in T cell biology, also regulates important innate inflammatory pathways in macrophages. Selective blocking of NFAT via a cell permeable inhibitory peptide is a promising therapeutic strategy for the treatment of inflammatory bowel diseases

Biodesalination: a case study for applications of photosynthetic bacteria in water treatment

Shortage of freshwater is a serious problem in many regions worldwide, and is expected to become even more urgent over the next decades as a result of increased demand for food production and adverse effects of climate change. Vast water resources in the oceans can only be tapped into if sustainable, energy-efficient technologies for desalination are developed. Energization of desalination by sunlight through photosynthetic organisms offers a potential opportunity to exploit biological processes for this purpose. Cyanobacterial cultures in particular can generate a large biomass in brackish and seawater, thereby forming a low-salt reservoir within the saline water. The latter could be used as an ion exchanger through manipulation of transport proteins in the cell membrane. In this article, we use the example of biodesalination as a vehicle to review the availability of tools and methods for the exploitation of cyanobacteria in water biotechnology. Issues discussed relate to strain selection, environmental factors, genetic manipulation, ion transport, cell-water separation, process design, safety, and public acceptance

Biodesalination: a case study for applications of photosynthetic bacteria in water treatment

Shortage of freshwater is a serious problem in many regions worldwide, and is expected to become even more urgent over the next decades as a result of increased demand for food production and adverse effects of climate change. Vast water resources in the oceans can only be tapped into if sustainable, energy-efficient technologies for desalination are developed. Energization of desalination by sunlight through photosynthetic organisms offers a potential opportunity to exploit biological processes for this purpose. Cyanobacterial cultures in particular can generate a large biomass in brackish and seawater, thereby forming a low-salt reservoir within the saline water. The latter could be used as an ion exchanger through manipulation of transport proteins in the cell membrane. In this article, we use the example of biodesalination as a vehicle to review the availability of tools and methods for the exploitation of cyanobacteria in water biotechnology. Issues discussed relate to strain selection, environmental factors, genetic manipulation, ion transport, cell-water separation, process design, safety, and public acceptance

'Biodesalination': a synthetic biology approach for the use of photosynthetic bacteria in water treatment

Shortage of freshwater is a serious global problem, and expected to become even more urgent over the next decades. Many of the driest regions worldwide are close to the sea, but irrigation of fields with seawater–even if diluted–leads to the build-up of salt levels in the soil that are toxic to all common food crops (http://www.unwater.org). Current desalination technologies such as membrane-based reverse osmosis, are successfully used in large-scale desalination plants, however, they are expensive and energy inefficient [1]. Our multi-disciplinary team of biologists and engineers from 5 UK universities is working on an innovative desalination technology based on biological processes [2]. The “Biodesalination” strategy envisions the use of photosynthetic cyanobacteria modified with light-driven ion transport proteins to function as ion exchangers that selectively remove sodium chloride from seawater. This process would harness solar energy to provide a more cost effective and energetically sustainable desalination process

'Biodesalination': a synthetic biology approach for the use of photosynthetic bacteria in water treatment

Shortage of freshwater is a serious global problem, and expected to become even more urgent over the next decades. Many of the driest regions worldwide are close to the sea, but irrigation of fields with seawater–even if diluted–leads to the build-up of salt levels in the soil that are toxic to all common food crops (http://www.unwater.org). Current desalination technologies such as membrane-based reverse osmosis, are successfully used in large-scale desalination plants, however, they are expensive and energy inefficient [1]. Our multi-disciplinary team of biologists and engineers from 5 UK universities is working on an innovative desalination technology based on biological processes [2]. The “Biodesalination” strategy envisions the use of photosynthetic cyanobacteria modified with light-driven ion transport proteins to function as ion exchangers that selectively remove sodium chloride from seawater. This process would harness solar energy to provide a more cost effective and energetically sustainable desalination process