Chlamydia psittaci st24: clonal strains of one health importance dominate in Australian horse, bird and human infections

Chlamydia psittaci is traditionally regarded as a globally distributed avian pathogen that can cause zoonotic spill-over. Molecular research has identified an extended global host range and significant genetic diversity. However, Australia has reported a reduced host range (avian, horse, and human) with a dominance of clonal strains, denoted ST24. To better understand the widespread of this strain type in Australia, multilocus sequence typing (MLST) and ompA genotyping were applied on samples from a range of hosts (avian, equine, marsupial, and bovine) from Australia. MLST confirms that clonal ST24 strains dominate infections of Australian psittacine and equine hosts (82/88; 93.18%). However, this study also found novel hosts (Australian white ibis, King parrots, racing pigeon, bovine, and a wallaby) and demonstrated that strain diversity does exist in Australia. The discovery of a C. psittaci novel strain (ST306) in a novel host, the Western brush wallaby, is the first detection in a marsupial. Analysis of the results of this study applied a multidisciplinary approach regarding Chlamydia infections, equine infectious disease, ecology, and One Health. Recommendations include an update for the descriptive framework of C. psittaci disease and cell biology work to inform pathogenicity and complement molecular epidemiologySusan I. Anstey, Vasilli Kasimov, Cheryl Jenkins, Alistair Legione, Joanne Devlin, Jemima Amery-Gale ... et al

Kualitas Hidup Pasien Diabetes Melitus Tipe 2 di Puskesmas Se Kota Kupang

Diabetes Mellitus is well known as a chronic disease which can lead to a decrease in quality of life in all domains. The study aims to explore the diabetic type 2 patient\u27s quality of life and find out the factors affecting in type 2 diabetic mellitus patients. The cross-sectional study design is used that included 65 patient with type 2 diabetes mellitus, in 11 public health centers of Kupang City. Data were collected by using Short Form Survey (SF-36) that assessed 8-scale health profile. Independent sample t-test is used to analyze the correlation between the factors affecting and the quality of life. the study showed that the QoL of DM patients decreased in all 8- health profile including physical functioning, social functioning, mental health, general health, pain, change in the role due to physical problems and emotional problems. The Study also showed there was a relationship between gender, duration of suffering from Diabetes mellitus, and complications to the quality of life. Male perceived a better quality of life than female

Bound, free and in between: a review of pronouns in Ngarrindjeri in the world as it was

Ngarrindjeri, a language from southern South Australia, is being revived on the basis of material recorded from 1840 until the 1960s. This material shows a heavy use of three types of pronouns, suggesting a language that is ‘pronoun happy’. When reviving a language, it is essential to know how pronouns work, but the earliest source does not include the kinds of texts that allow analysis of how speakers use pronouns. Texts representing actual connected free speech in Ngarrindjeri are not attested until nearly a century later, by Ronald and Catherine Berndt and Norman Tindale. We compare the forms, meanings and second position distribution of Ngarrindjeri pronouns over time and across sources, considering dialect variation and language change. We show that the pronoun form-meaning pairs in texts recorded in the 1930s and 1940s are consistent with those recorded in the nineteenth century, and so we can have some confidence in using the Berndt and Tindale texts to reconstruct pronoun function. Confidence is further enhanced by showing the similarity in pronoun functions in texts recorded on the same topic from the same speaker, Albert Karloan, by the Berndts and Tindale. This review of Ngarrindjeri pronouns opens up possibilities for language revivalists.Mary-Anne Gale, Rob Amery, Jane Simpson and David Wilkin

What women want: Teaching and learning pronouns in Ngarrindjeri

Ngarrindjeri is one of many Aboriginal languages being actively revived in southern Australia. Women in the Ngarrindjeri community have expressed a desire to speak, read and write their language with the same richness as when it was spoken fluently over 70 years ago. Like many Aboriginal languages, Ngarrindjeri has a rich selection of free and bound pronouns, which express person, number and case, but unlike most other Australian languages, it has a third set of reduced free form pronouns. This tripartite set is used to express discourse saliency and continuing topic, and to definitize noun phrases. This paper addresses the issue of teaching and learning how to use Ngarrindjeri pronouns in traditional ways, but for contemporary purposes. Learning Ngarrindjeri requires understanding grammatical categories such as case that differ substantially from English, plus understanding the use of free forms for discourse saliency, bound forms for continuing topics, and free reduced forms where English uses articles. Finally, it requires memorizing a large number of pronoun forms. We share anecdotes on learning pronouns from individual authors, and a reflection from a young Ngarrindjeri woman. We then propose strategies and resources to make it easier to learn, remember and use the complex, regularized pronoun paradigms of Ngarrindjeri.Mary-Anne Gale, Angela Giles, Jane Simpson, Rob Amery and David Wilkin

A high prevalence of beak and feather disease virus in non-psittacine Australian birds

PURPOSE: Beak and feather disease virus (BFDV) is a circovirus and the cause of psittacine beak and feather disease (PBFD). This disease is characterized by feather and beak deformities and is a recognized threat to endangered Psittaciformes (parrots and cockatoos). The role that non-psittacine birds may play as reservoirs of infection is unclear. This study aimed to begin addressing this gap in our knowledge of PBFD. METHODOLOGY: Liver samples were collected from birds presented to the Australian Wildlife Health Centre at Zoos Victoria's Healesville Sanctuary for veterinary care between December 2014 and December 2015, and tested for BFDV DNA using polymerase chain reaction coupled with sequencing and phylogenetic analyses.Results/Key findings. Overall BFDV was detected in 38.1 % of 210 birds. BFDV was detected at high prevalence (56.2 %) in psittacine birds, in the majority of cases without any observed clinical signs of PBFD. We also found that BFDV was more common in non-psittacine species than previously recognized, with BFDV detected at 20.0 % prevalence in the non-psittacine birds tested, including species with no clear ecological association with psittacines, and without showing any detectable clinical signs of BFDV infection. CONCLUSION: Further research to determine the infectivity and transmissibility of BFDV in non-psittacine species is indicated. Until such work is undertaken the findings from this study suggest that every bird should be considered a potential carrier of BFDV, regardless of species and clinical presentation. Veterinary clinics and wildlife rehabilitation facilities caring for birds that are susceptible to PBFD should reconsider biosecurity protocols aimed at controlling BFDV

Recent history of psittacosis in Australia: expanding our understanding of the epidemiology of this important globally distributed zoonotic disease

Psittacosis is a human systemic disease caused by infection with Chlamydia psittaci. Shortly after reports emerged of a global pandemic associated with contact with imported parrots, Australian researchers including Macfarlane Burnet and others demonstrated that C. psittaci was widespread in Australian parrots. Australian cases over the last two decades have revealed that environmental exposure and contact with infected horses are also risk factors in an increasingly complicated epidemiological picture for this zoonotic disease

Eimeria taggarti n. sp., a novel coccidian (Apicomplexa: Eimeriorina) in the prostate of an Antechinus flavipes

A novel coccidian species was discovered in the prostate of an Antechinus flavipes (yellow-footed antechinus) in South Australia, during the period of post-mating male antechinus immunosuppression and mortality. This novel coccidian is unusual because it develops extra-intestinally and sporulates endogenously within the prostate gland of its mammalian host. Histological examination of prostatic tissue revealed dense aggregations of spherical and thin-walled tetrasporocystic, dizoic sporulated coccidian oocysts within tubular lumina, with unsporulated oocysts and gamogonic stages within the cytoplasm of glandular epithelial cells. This coccidian was observed occurring concurrently with dasyurid herpesvirus 1 infection of the antechinus' prostate. Eimeria-specific 18S small subunit ribosomal DNA PCR amplification was used to obtain a partial 18S rDNA nucleotide sequence from the antechinus coccidian. Bayesian phylogenetic analysis based on 18S rDNA gene sequences revealed that the novel coccidian clusters with reptile-host coccidians, forming an ancestral basal lineage of the eimeriid clade. The species has been named Eimeria taggarti n. sp., based on both sporulated oocyst morphology and molecular characterization. It is suspected that E. taggarti is sexually transmitted via excretion of sporulated oocysts and/or free sporocysts with prostatic secretions in semen.J. Amery-Gale, J.M. Devlin, L. Tatarczuch, D.A. Taggart, D.J. Schultz, J.A. Charles and I. Beveridg

Avian viral surveillance in Victoria, Australia, and detection of two novel avian herpesviruses

Viruses in avian hosts can pose threats to avian health and some have zoonotic potential. Hospitals that provide veterinary care for avian patients may serve as a site of exposure of other birds and human staff in the facility to these viruses. They can also provide a useful location to collect samples from avian patients in order to examine the viruses present in wild birds. This study aimed to investigate viruses of biosecurity and/or zoonotic significance in Australian birds by screening samples collected from 409 birds presented to the Australian Wildlife Health Centre at Zoos Victoria's Healesville Sanctuary for veterinary care between December 2014 and December 2015. Samples were tested for avian influenza viruses, herpesviruses, paramyxoviruses and coronaviruses, using genus- or family-wide polymerase chain reaction methods coupled with sequencing and phylogenetic analyses for detection and identification of both known and novel viruses. A very low prevalence of viruses was detected. Columbid alphaherpesvirus 1 was detected from a powerful owl (Ninox strenua) with inclusion body hepatitis, and an avian paramyxovirus most similar to Avian avulavirus 5 was detected from a musk lorikeet (Glossopsitta concinna). Two distinct novel avian alphaherpesviruses were detected in samples from a sulphur-crested cockatoo (Cacatua galerita) and a tawny frogmouth (Podargus strigoides). Avian influenza viruses and avian coronaviruses were not detected. The clinical significance of the newly detected viruses remains undetermined. Further studies are needed to assess the host specificity, epidemiology, pathogenicity and host-pathogen relationships of these novel viruses. Further genome characterization is also indicated, and would be required before these viruses can be formally classified taxonomically. The detection of these viruses contributes to our knowledge on avian virodiversity. The low level of avian virus detection, and the absence of any viruses with zoonotic potential, suggests low risk to biosecurity and human health