Identification and validation of a QTL influencing bitter pit symptoms in apple (Malus x domestica)

Bitter pit is one of the most economically important physiological disorders affecting apple fruit production, causing soft discrete pitting of the cortical flesh of the apple fruits which renders them unmarketable. The disorder is heritable; however, the environment and cultural practices play a major role in expression of symptoms. Bitter pit has been shown to be controllable to a certain extent using calcium sprays and dips; however, their use does not entirely prevent the incidence of the disorder. Previously, bitter pit has been shown to be controlled by two dominant genes, and markers on linkage group 16 of the apple genome were identified that were significantly associated with the expression of bitter pit symptoms in a genome-wide association study. In this investigation, we identified a major QTL for bitter pit defined by two microsatellite (SSR) markers. The association of the SSRs with the bitter pit locus, and their ability to predict severe symptom expression, was confirmed through screening of individuals with stable phenotypic expression from an additional mapping progeny. The data generated in this current study suggest a two gene model could account for the control of bitter pit symptom expression; however, only one of the loci was detectable, most likely due to dominance of alleles carried by both parents of the mapping progeny used. The SSR markers identified are cost-effective, robust and multi-allelic and thus should prove useful for the identification of seedlings with resistance to bitter pit using marker-assisted selection in apple breeding programs

Storage of ‘Laetitia’ plums (Prunus salicina) under controlled atmosphere conditions

The optimum condition for storage of 'Laetitia' plums (Prunus salicina) under controlled atmosphere (CA) is not currently known. This study was aimed at assessing the effects of controlled atmospheric (CA) conditions on the postharvest quality preservation of ‘Laetitia’ plums. Besides regular atmosphere  (RA; 21kPa O2 + 0.03 kPa CO2) as condition/treatment control, the following CA conditions (kPa O2 + kPa CO2) were assessed: 1+3; 1+5; 2+5; 2+10; and 11+10. In all cases, the fruit were stored for 60 days at 0.5°C±0.1°C and 96±2% of relative humidity (RH). Upon removal from the cold storage chamber and after four days in ambient conditions (20±2°C/60±5% RH), the fruit were assessed in terms of: respiration and ethylene production rates; flesh firmness; texture; titrable acidity; red color index and hue angle (ho) of the skin; incidence of fruit cracking and internal breakdown (flesh browning). The fruit stored under the different CA conditions presented lower respiration and ethylene production rates, higher values of flesh firmness, texture and titrable acidity, lower development of skin red color, and lower incidence of skin cracking compared to the fruit under RA. CA conditions of 2+5, 1+5, and 1+3 resulted in a more substantial delay of ripening. CA conditions of 2+5 and 1+3 resulted in lower incidence of internal breakdown.Keywords: Ripening, physiological disorder, postharvest, Prunus salicina.African Journal of Biotechnology, Vol 13(31) 3239-324

Eficiência de acessos de Cucurbita maxima como polinizadores de abóbora híbrida do tipo "Tetsukabuto" Efficiency of Cucurbita maxima accessions as pollinators in the commercial production of pumpkin ("Tetsukabuto" type)

A abóbora tipo “Tetsukabuto” é um híbrido interespecífico, macho estéril, resultante do cruzamento entre Cucurbita maxima e C. moschata. Uma das estratégias utilizadas para produção comercial de frutos de “Tetsukabuto” tem sido o plantio adjacente e concomitante de acessos de C. maxima ou C. moschata como polinizadores. Este trabalho teve por objetivo avaliar a eficiência de diferentes acessos de C. maxima como fontes de pólen para produção comercial de abóbora tipo “Tetsukabuto”. A cultivar de moranga "Exposição" e o acesso "Nirvana" foram utilizados como polinizadores para produção de frutos de dois híbridos do tipo varietal “Tetsukabuto” ("Jabras" e "Kyoto"). Foram avaliados os tratamentos (cruzamentos) Jabras x Exposição, Jabras x Nirvana, Tetsukabuto x Exposição, Tetsukabuto x Nirvana, em quatro repetições. Foram determinados os parâmetros massa dos frutos, diâmetro dos frutos, espessura da polpa, número e massa de sementes. O acesso "Nirvana" (cultivar em fase de validação) pode ser utilizado na produção comercial como polinizador de abóboras deste grupo varietal sem nenhum prejuízo quando comparado com a moranga "Exposição".<br>"Tetsukabuto" pumpkins are interspecific hybrids between Cucurbita maxima x C. moschata accessions. Such hybrids, however, are often male-sterile due to the impaired ability of the staminate flowers to produce functional pollen. The use of intervening rows of staminate (pollen-donor) plants is one strategy employed in order to have commercial fruit production. In the present work, two C. maxima accessions ["Exposição" (E) and "Nirvana" (N)] were employed as pollen-donors (staminate parents) and their efficiency was compared considering fruit and seed yield parameters. Two pistillate Tetsukabuto hybrids ["Jabras" (J) and "Kyoto Tetsukabuto" (KT)] were employed in four possible unidirectional crosses (J x E, J x N, KT x E and KT x N). Fruits were produced using standard manual pollination techniques done on a daily basis (during the morning). After harvesting, the following traits were evaluated: fruit weight, fruit diameter, fruit flesh depth, number of seeds per fruit and total seed weight. The results indicated that both staminate parents are equivalent for all traits. The accession "Nirvana" (a cultivar in a pre-release stage) could be considered a commercial alternative to "Exposição" (the traditional staminate parent under Brazilian conditions) due to its improved fruit quality attributes, which could provide an additional source of revenues for the "Tetsukabuto" growers