Use of \u3cem\u3eN\u3c/em\u3e-Alkane Technique to Estimate Sheep Dry Matter Intake

Given the complexity of evaluating intake on grazing, some compounds have been studied to promote qualitative and quantitative estimates of animal physiology. In this sense, the technique using n-alkanes as a marker has been used in several animal species, especially in grazing ruminants (Dove and Mayes 1996). By definition, validation under grazing or browsing conditions is not possible, because actual intakes are unknown (Dove and Mayes 2005). Thus, the aim of the study was to evaluate the methodology of n-alkanes to estimate herbage intake by sheep in metabolic cages

Decreased expression of ADAMTS-1 in human breast tumors stimulates migration and invasion

Abstract\ud \ud \ud \ud Background\ud \ud ADAMTS-1 (a disintegrin and metalloprotease with thrombospondin motifs) is a member of the ADAMTS family of metalloproteases. Here, we investigated mRNA and protein levels of ADAMTS-1 in normal and neoplastic tissues using qPCR, immunohistochemistry and immunoblot analyses, and we addressed the role of ADAMTS-1 in regulating migration, invasion and invadopodia formation in breast tumor cell lines.\ud \ud \ud \ud Results\ud \ud In a series of primary breast tumors, we observed variable levels of ADAMTS-1 mRNA expression but lower levels of ADAMTS-1 protein expression in human breast cancers as compared to normal tissue, with a striking decrease observed in high-malignancy cases (triple-negative for estrogen, progesterone and Her-2). This result prompted us to analyze the effect of ADAMTS-1 knockdown in breast cancer cells in vitro. MDA-MB-231 cells with depleted ADAMTS-1 expression demonstrated increased migration, invasion and invadopodia formation. The regulatory mechanisms underlying the effects of ADAMTS-1 may be related to VEGF, a growth factor involved in migration and invasion. MDA-MB-231 cells with depleted ADAMTS-1 showed increased VEGF concentrations in conditioned medium capable of inducing human endothelial cells (HUVEC) tubulogenesis. Furthermore, expression of the VEGF receptor (VEGFR2) was increased in MDA-MB-231 cells as compared to MCF7 cells. To further determine the relationship between ADAMTS-1 and VEGF regulating breast cancer cells, MDA-MB-231 cells with reduced expression of ADAMTS-1 were pretreated with a function-blocking antibody against VEGF and then tested in migration and invasion assays; both were partially rescued to control levels.\ud \ud \ud \ud Conclusions\ud \ud ADAMTS-1 expression was decreased in human breast tumors, and ADAMTS-1 knockdown stimulated migration, invasion and invadopodia formation in breast cancer cells in vitro. Therefore, this series of experiments suggests that VEGF is involved in the effects mediated by ADAMTS-1 in breast cancer cells.This investigation was supported by The State of São Paulo Research Foundation (FAPESP grants 2006/54963-0, 2006/01026-0, 2008/57103-8, 2010/07699-1), and Brazilian National Council for Scientific and Technological Development (CNPq grant 470779/2007-1). The authors also want to thank Dr. Stanley Zucker (Stony Brook University; Stony Brook, NY, USA) and Dr. Rama Khokha (University of Western Ontario, Toronto, Canada) for suggestions made on data analysis

Diet Switching by Mammalian Herbivores in Response to Exotic Grass Invasion.

Invasion by exotic grasses is a severe threat to the integrity of grassland ecosystems all over the world. Because grasslands are typically grazed by livestock and wildlife, the invasion is a community process modulated by herbivory. We hypothesized that the invasion of native South American grasslands by Eragrostis plana Nees, an exotic tussock-forming grass from Africa, could be deterred by grazing if grazers switched dietary preferences and included the invasive grass as a large proportion of their diets. Bos taurus (heifers) and Ovis aries (ewes) grazed plots with varying degrees of invasion by E. plana in a replicated manipulative experiment. Animal positions and species grazed were observed every minute in 45-min grazing session. Proportion of bites and steps in and out of E. plana tussocks were measured and used to calculate several indices of selectivity. Both heifers and ewes exhibited increasing probability of grazing E. plana as the proportion of area covered by tussocks increased, but they behaved differently. In agreement with expectations based on the allometry of dietary preferences and morphology, ewes consumed a low proportion of E. plana, except in areas that had more than 90% E. plana cover. Heifers consumed proportionally more E. plana than ewes. Contrary to our hypothesis, herbivores did not exhibit dietary switching towards the invasive grass. Moreover, they exhibited avoidance of the invasive grass and preference for short-statured native species, both of which should tend to enhance invasion. Unless invasive plants are highly palatable to livestock, the effect of grazing to deter the invasion is limited, due to the inherent avoidance of the invasive grass by the main grazers in the ecosystem, particularly sheep

Partial selectivity levels (mean ± standard error) for inter-tussock stratum by ewes.

<p>The continuous line represents the feeding station to bite scale and the dashed line represents the search path or patch scale. Values >1.0 mean that the inter-tussock was selected more than expected by random encounter.</p

Wald’s Chi-sq tests for terms in the model for probability of biting intra-tussock in ewes.

<p>Wald’s Chi-sq tests for terms in the model for probability of biting intra-tussock in ewes.</p

Wald’s Chi-sq tests for terms in the model for probability of biting tussock in heifers.

<p>Wald’s Chi-sq tests for terms in the model for probability of biting tussock in heifers.</p

Wald’s Chi-sq tests for terms in the model for probability of biting tussock in ewes.

<p>Wald’s Chi-sq tests for terms in the model for probability of biting tussock in ewes.</p

F tests for terms in the model of selectivity of ewes as a function of plot-level treatment and scale of selectivity.

<p>F tests for terms in the model of selectivity of ewes as a function of plot-level treatment and scale of selectivity.</p

Histograms of tussock cover measured in a 1-m² grid in each of the treatments applied at the whole plot or paddock level (0, 25, 50 and 75% of <i>Eragrostis plana</i> Nees.) Spatial variability in tussock cover resulted in a variety of values observed within each treatment.

<p>Histograms of tussock cover measured in a 1-m² grid in each of the treatments applied at the whole plot or paddock level (0, 25, 50 and 75% of <i>Eragrostis plana</i> Nees.) Spatial variability in tussock cover resulted in a variety of values observed within each treatment.</p