Investigations on microbiome of the used clinical device revealed many uncultivable newer bacterial species associated with persistent chronic infections

Introduction. Chronic persistent device-related infections (DRIs) often give culture-negative results in a microbiological investigation. In such cases, investigations on the device metagenome might have a diagnostic value. Materials and Methods. The 16SrRNA gene sequence analysis and next-generation sequencing (NGS) of clinical metagenome were performed to detect bacterial diversity on invasive medical devices possibly involved in culture-negative DRIs. Device samples were first subjected to microbiological investigation followed by metagenome analysis. Environmental DNA (e-DNA) isolated from device samples was subjected to 16SrRNA gene amplification followed by Sanger sequencing (n=14). In addition, NGS of the device metagenome was also performed (n=12). Five samples were only common in both methods. Results. Microbial growth was observed in only nine cases; among these, five cases were considered significant growth, and in the remaining four cases, growth was considered either insignificant or contaminated. Culture and sequencing analysis yielded identical results only in six cases. In culture-negative cases, Sanger sequencing of 16SrRNA gene and NGS of 16SrDNA microbiome was able to identify the presence of rarely described human pathogens, namely Streptococcus infantis, Gemella haemolysans, Meiothermus silvanus, Schlegelella aquatica, Rothia mucilaginosa, Serratia nematodiphila, and Enterobacter asburiae, along with some known common nosocomial pathogens. Bacterial species such as M. silvanus and S. nematodiphila that are never reported in human infection were also identified. Conclusions. Results of a small number of diverse samples of this pilot study might lead to a path to study a large number of device samples that may validate the diversity witnessed. The study shows that a culture free, a holistic metagenomic approach using NGS could help identify the pathogens in culture-negative chronic DRIs

PANC Study (Pancreatitis: A National Cohort Study): national cohort study examining the first 30 days from presentation of acute pancreatitis in the UK

Abstract Background Acute pancreatitis is a common, yet complex, emergency surgical presentation. Multiple guidelines exist and management can vary significantly. The aim of this first UK, multicentre, prospective cohort study was to assess the variation in management of acute pancreatitis to guide resource planning and optimize treatment. Methods All patients aged greater than or equal to 18 years presenting with acute pancreatitis, as per the Atlanta criteria, from March to April 2021 were eligible for inclusion and followed up for 30 days. Anonymized data were uploaded to a secure electronic database in line with local governance approvals. Results A total of 113 hospitals contributed data on 2580 patients, with an equal sex distribution and a mean age of 57 years. The aetiology was gallstones in 50.6 per cent, with idiopathic the next most common (22.4 per cent). In addition to the 7.6 per cent with a diagnosis of chronic pancreatitis, 20.1 per cent of patients had a previous episode of acute pancreatitis. One in 20 patients were classed as having severe pancreatitis, as per the Atlanta criteria. The overall mortality rate was 2.3 per cent at 30 days, but rose to one in three in the severe group. Predictors of death included male sex, increased age, and frailty; previous acute pancreatitis and gallstones as aetiologies were protective. Smoking status and body mass index did not affect death. Conclusion Most patients presenting with acute pancreatitis have a mild, self-limiting disease. Rates of patients with idiopathic pancreatitis are high. Recurrent attacks of pancreatitis are common, but are likely to have reduced risk of death on subsequent admissions. </jats:sec

Floating Drug Delivery System an Aid to Enhance Dissolution Profile of Gastric

With the GRDDS, the dose shape remains controllably in the stomach after oral administration, so that the medication may be continually delivered to its absorption receptors in the intestinal tract. The medicine is delivering in a controlled and extended way. Gastro-retentive dose in the stomach area may last for another few hours and substantially lengthen the gastric residence period of the medicines. While the bulk density in the system for the supply of floating medicines (FDDS) exceeds the gastric fluids, it remains for an extended duration in the stomach without altering the rate of decomposition. The medication distributes gradually as the system floats on the stomach juice. As a consequence, stomach residency takes longer and plasma concentrations are well monitored. The therapy of peptic ulcer illness might be beneficial for local activity in the upper portion of the intestine, i.e., a longer stomach residency. In addition, medicines rapidly absorbed in the GI tract will increase bioavailability through delayed stomach release. The regulated gastric retention of solid dose forms can also be accomplished by the simultaneous administration of pharmacological agents, or by sedimentation, flotation processes, muco-adhesion, expansion, changed shape systems, by delaying the stomach emptying. Keywords: Gastro-retentive drug delivery system, Floating drug delivery system, Muco-adhesion, Bioavailability

Effect of Physico-Chemical Properties of Soil under Various Depths on Different Cropping Systems in Ayodhya, India

A research work conducted during 2018-19 at Agronomy farm of Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya (U.P.) to evaluate the fertility status and effect of different cropping systems on soil physico-chemical characteristics and their correlation. The 300 representative soil samples with 4 depths viz. 0-15, 15-30, 30-60 and 60-90 cm soil samples were taken with manually driven post hole auger and processed for soil analysis from different plots of Agronomy farm. Results revealed that physico-chemical properties viz. particle Density, bulk density pH, EC, organic corban, nitrogen phosphorus potassium Sulphur and zinc varied vertically and horizontally in cultivated and fallow lands. The soils were sandy loam, clay loam, loam and silt loam.&nbsp; The frequency distribution of soil bulk density of entire farm ranged from 1.128-1.58 Mgm-3, particle density ranged 2.50-2.76 Mgm-3 and soil chemical properties, like, pH&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; ranged from 7.2-11.5, the E.C. is found in ranged from .13-1.01 dSm-1. While organic carbon was ranged from 1.4-6.70 g kg-1. and notable available Nitrogen in Agronomy farm was low in ranged from 170.15-221.36 kgha-1.The Phosphorus availability was found in ranged from 6.01-16.87 kgha-1 , while Potassium was found in ranged from 163.20-220.98 kgha-1. How ever the availability of Sulphur, was found in ranged from 8.62-18.32 kgha-1, Zinc ranged from in Agronomy farm 0.28-0.81mgkg-1. The significant positive correlation were observed among soil B.D with N, B.D with P, B.D with K, B.D with S, P.D. with S, P.D. with Zn. While the significant negative correlations were observed among PD with O.C., Zn with P, Zn with K and Zn with S in whole Agronomy Farm

Effect of Phosphorus Level on Nutrient Availability and Economics of Potato Crop (Solanum tuberosum L.)

The experiment was conducted at Narendra Deva University of Agriculture and Technology's Main Experiment Station, Department of Vegetable Science, Narendra Nagar (Kumarganj), ayodhya (U.P.), during the Rabi season of 2016–17. The soil in the experimental field has a sandy loam texture. Four replications of a Randomized Block Study were applied to seven treatments. The experimental findings showed that the use of RDF 100 kg P2O5 per ha was found better with respect to promotion of growth, yield and quality parameters of potato. The maximum value on growth characters were also recorded T7 -RDF kg P2O5 ha-1 is better as compare to (T5-90 kg P2O5 ha-1 and T6-120 kg P2O5 ha-1) using in potato variety Kufri Khyati. It was found significantly superior over rest of all the treatments. An application of treatment T7 -RDF 100 kg P2O5 ha-1 in was found to be most effective total phosphorus uptake by plant and tuber (24.31kg h-1) and available phosphorus (13.50 kg h-1). The tuber and plant content of phosphorus was found T7 (.280 %) and (0.156%) and dry matter (18.10%) tuber and (10.86%) in plant for highest tubers yield (388.79 q per ha) and maximum net return Rs. 139062 as benefit cost ratio 1.47 on this dose of phosphorus in the years investigation

A Review: Pulses Production, Productivity, Status and Way Forward for Enhancing Farmers Income of Bundelkhand Region, India

Total pulses area in Uttar Pradesh, contributes of pulses through the Bundelkhand region, more than half but the productivity is low because lack of technological approaches for pulse growing area. This review paper assessed the cultivation of pulses production, productivity and area, of Bundelkhand and in India also. The technologies and infrastructure need to be accepted pulses cultivation and accurate policies for the farmers through government.&nbsp; Pulses are next to cereals regarding their nutritional and economic significance to human health. The pulses crop highly water sensitive like chickpea, lentil, mung bean and black gram are dominant in drought prone areas and improve soil fertility by fixing atmospheric nitrogen. Rainfed Agriculture has emerged as an opportunity in raising pulse production which is depend on amount and distribution of rainfall growing area. The cropping system of pulses cultivation year-after-year has observed that incidence of disease, insect-pests and weeds increase in Bundelkhand region. In pigeon pea, chickpea and lentil crops, important biotic agent for affecting production. United Nations declared 2016 ' "International Year of Pulses" was to increase production and consumption of pulses 10% by 2025 and raise public awareness towards health through social media. The many initiatives schemes govern by government of India for increase the pulses growing area and production such as ISOPOM (Integrated Scheme of Oilseeds, Pulses, Oil palm and Maize) (2004-05 to 2009-10), NFSM Pulses (National Food Security Mission, 2007-08), for Technology demonstration. The Bundelkhand regions as 5 lakhs farmers of 14 districts of two states of Madhya Pradesh and Uttar Pradesh should be linked with Rani Lakshmi Bai Central Agricultural University (RLBCAU) and benefited from this university to produce pulses

Effect of Various Organic and Inorganic Sources of Nutrients on Growth, Yield, and Economics of Kharif Greengram [Vigna radiata. (L.) Wilczek] in the Bundelkhand Region, India

The application of organics alone does not result in a dramatic rise in crop yields due to their low nutritional status and large quantity demand, which is also a restriction. As a result of the aforementioned implications, Greengram may now be grown using a combination of organic and inorganic fertilizers, as well as bio-fertilizers. There for a felid experiment was conducted in&nbsp; Effect of various organic and inorganic sources of nutrients on growth, yield, and economics of Kharif Greengram [Vigna radiata. (L.) Wilczek] in the Bundelkhand Region.The experiment was laid out in randomized block design with 3 replication having 10 treatments. The&nbsp; application of Rhizobiumculture @ 25g/kg of seed along withVermicompost @ 2.5 t ha-1; FYM @5 t ha-1and 100% RDF was found significantly superior in plant height (69.90cm), number of leaves per plant (60.50), leaf area index (1.88) and Number of Rhizobium nodules plant-1 (123.37) at 45 DAS, number of branches (8.43), Plant dry matter (362.11 g m-2), pods plant-1 (28.60), grain pod-1 (11.20), Length of pods (9.87cm), Seed index (4.50g), grain yield (11.41q ha-1), and straw yield (27.45q ha-1), Gross monetary returns(82330 Rs ha-1) were found higher in same treatment T10, as compared to other combination and control. However, highest benefit: cost ratio (3.44) was recorded with use of Rhizobium culture and application of 100% RDF (T2) as compared to other combination and control

Proceedings of the International Conference on Frontiers in Desalination, Energy, Environment and Material Sciences for Sustainable Development

This proceeding contains articles on the various ideas of the academic community presented at the International Conference on Frontiers in Desalination, Energy, Environment and Material Sciences for Sustainable Development (FEEMSSD-2023) &amp; Annual Congress of InDA (InDACON-2023) jointly organized by the Madan Mohan Malaviya University of Technology Gorakhpur, KIPM-College of Engineering and Technology Gida Gorakhpur, and Indian Desalination Association, India on 16th-17th March 2023.  FEEMSSD-2023 &amp; InDACON-2023 focuses on addressing issues and concerns related to sustainability in all domains of Energy, Environment, Desalination, and Material Science and attempts to present the research and innovative outputs in a global platform. The conference aims to bring together leading academicians, researchers, technocrats, practitioners, and students to exchange and share their experiences and research outputs in Energy, Environment, Desalination, and Material Science.  Conference Title: International Conference on Frontiers in Desalination, Energy, Environment and Material Sciences for Sustainable Development &amp; Annual Congress of InDAConference Acronyms: FEEMSSD-2023 &amp; InDACON-2023Conference Date: 16th-17th March 2023Conference Location: Madan Mohan Malaviya University of Technology, GorakhpurConference Organizers: Madan Mohan Malaviya University of Technology Gorakhpur, KIPM-College of Engineering and Technology Gida Gorakhpur, and Indian Desalination Association, Indi