9 research outputs found
Molluscicidal activity of Bacillus thuringiensis strains against Biomphalaria alexandrina snails
Schistosomiasis is a parasitic disease transmitted to man and different warm blooded animals by means of snails. Great effort has been made to control the transmission of the disease by many strategies. Consequently, the utilization of particular molluscicides is viewed as a standout amongst the best measures for molluscs control. Recently, microbial pathogen used as non-traditional molluscicides which have attracted significant research attention due to the increasing, worldwide development of resistance to chemical molluscicides in molluscs populations. The present work aimed to study the molluscicidal impacts of eleven isolates of Bacillus thuringiensis which were isolated from soils of six Egyptian governorates toward Biomphalaria alexandrina snails. Results showed that, B. thuringiensis provides an effective biological control agent against B. alexandrina snails. Out of the tested isolates, four isolates; Qalyubia, Asyut 1, Qena and North Sinai 2 isolates show high-level molluscicidal activity. The obtained results indicated that LC50 and LC90 values were ranged between 133.27â457.74Â mg/mL and 270.32â781.05Â mg/mL, respectively. The most noteworthy molluscicidal impact was displayed by Qalyubia isolate which isolated from Qalyubia governorate with mortality rate extended from 20% to100% at five treatment concentrations of 100â500Â mg/mL. The LC50 and LC90 values for Qalyubia isolate were 133.27Â mg/mL and 270.32Â mg/mL, respectively. Keywords: Biomphalaria alexandrina, Bacillus thuringiensis, Molluscicidal activity, Biological contro
Giardia lamblia in household persons and buffalo calves; prevalence, molecular identification and associated risk factors
Occurrence and Public Health Importance of Ascaridoid Nematodes in Red Porgy, Pagrus pagrus (Perciformes: Sparidae)
Anisakid nematodes have been found in a variety of marine fishes worldwide and they are known to cause anisakiasis and allergic symptoms in human. One-hundred and five fish specimens of different sizes of red porgy (Pagrus pagrus) were collected randomly from Sharkia fish markets originating from the Gulf of Suez, Red Sea, Egypt and examined for the presence of ascaridoid nematodes. The overall prevalence of ascaridoids was 36.19% (38/105). Four morphotypes belonging to two genera of anisakid nematodes (Anisakis type I and Terranova type II larvae) as well as one genus of raphidascarid; Hysterothylacium (larvae and adult) were reported during this study. Hysterothylacium larvae were the most predominant species (23.81%) and abundance value =1.68. Interestingly, Pagrus pagrus was found as new host and locality records for larvae of Terranova type II. The results revealed a significant statistical correlation between fish length and number of the recovered ascaridoid nematodes (rs= 0.244, P < 0.05). There was a positive correlation between the host length and presence of Anisakis type I L3 (rs=0.276, P < 0.001). Significant differences were observed between the occurrence of Terranova type II L3 and Hysterothylacium L3 occurrence (rs= 0.388, P < 0.001) and adult (rs= 0.442, P < 0.001). In addition, a highly significant relationship was observed between the presence of Hysterothylacium L3 and adult (rs= 0.428, P < 0.001). Furthermore, the obtained results were lower than the recommended Egyptian standards (˂100 parasites/fish), demonstrating that human health risk through consumption of fish flesh is not possible
Assessment of serum level of corticotropin-releasing factor in primary nocturnal enuresis
Microwave-Assisted Synthesis, Biological Activity Evaluation, Molecular Docking, and ADMET Studies of Some Novel Pyrrolo [2,3-b] Pyrrole Derivatives
Novel pyrrolo [2,3-b] pyrrole derivatives were synthesized and their hypolipidemic activity was assessed in hyperlipidemic rats. The chemical structures of the new derivatives were confirmed through spectral analysis. Compounds 5 and 6 were revealed to be the most effective hypolipidemic agents, with considerable hypocholesterolemic and hypotriglyceridemic effects. They appear to be promising candidates for creating new powerful derivatives with anti-atherosclerotic and hypolipidemic properties. As for antimicrobial activity, some of the tested compounds showed moderate activity against Pseudomonas aeruginosa: compound 2 revealed an MIC value of 50 μg/mL, compared to 25 μg/mL for ciprofloxacin. Compound 3 showed good antimicrobial activity against Staphylococcus aureus, comparable to ciprofloxacin, and roughly half the activity of ampicillin, according to MIC values. Compound 2 has an MIC approximately 25% of that of clotrimazole against Candida albicans. Compound 2 also showed the highest antioxidant activity with 59% inhibition of radical scavenging activity. Additionally, the cytotoxic activity of these new derivatives 1–7 was investigated and most of them showed good anticancer activity against the three tested cell lines
Thieno[2,3‑<i>b</i>]thiophene Derivatives as Potential EGFR<sup>WT</sup> and EGFRT<sup>790M</sup> Inhibitors with Antioxidant Activities: Microwave-Assisted Synthesis and Quantitative In Vitro and In Silico Studies
Microwave-assisted synthesis and spectral analysis of
certain novel
derivatives of 3,4-diaminothieno[2,3-b]thiophene-2,5-dicarbonitrile 1–7 were carried out. Compounds 1–7 were examined for cytotoxicity against MCF-7 and A549 cell lines
using the quantitative MTT method, and gefitinib and erlotinib were
used as reference standards. Compounds 1–7 were
shown to be more active than erlotinib against the two cell lines
tested. Compound 2 outperformed regular erlotinib by
4.42- and 4.12-fold in MCF-7 and A549 cells, respectively. The most
cytotoxic compounds were subsequently studied for their suppression
of kinase activity using the homogeneous time-resolved fluorescence
assay versus epidermal growth factor receptor (EGFRWT)
and EGFR790M. With IC50 values of 0.28 ±
0.03 and 5.02 ± 0.19, compound 2 was demonstrated
to be the most effective against both forms of EGFR. Furthermore,
compound 2 also had the best antioxidant property, decreasing
the radical scavenging activity by 78%. Molecular docking research,
on the other hand, was carried out for the analyzed candidates (1–7) to study their mechanism of action as EGFR inhibitors.
In silico absorption, distribution, metabolism, excretion, and toxicity
tests were also performed to explain the physicochemical features
of the examined derivatives