α-sinucleína, gotas lipídicas y un interactoma sin resolver

La α- sinucleína (α- sin) es una proteína citosólica pequeña, cuya agregación y fibrilación están estrechamente asociadas a un grupo de patologías degenerativas llamadas sinucleinopatías. Diferentes estudios han demostrado que esta proteína posee una alta afinidad de unión a lípidos, y a su vez se ha propuesto que actuaría como moduladora del metabolismo lipídico.Fil: Alza, N.. Consejo Nacional de Investigaciones Científicas y TécnicasFil: Conde, M.. Consejo Nacional de Investigaciones Científicas y TécnicasFil: Scodelaro Bilbao, P.. Consejo Nacional de Investigaciones Científicas y TécnicasFil: González Pardo, M.. Consejo Nacional de Investigaciones Científicas y TécnicasFil: Salvador, G.. Consejo Nacional de Investigaciones Científicas y Técnica

Soy protein modulates the effects of intoxication with cadmiun in rat mammary gland

We studied the effects of different diets at molecular, biochemical and cellular levels on rat mammary gland (MG). We looked at the potential protective outcome of those diets under exposure to Cadmiun (Cd), which is an important environmental contaminant. For this purpose, 4 female lots Wistar rats were used: 2 lots received casein and 2 lots soybean (Soy) as protein source. Within each group, 1 lot received regular water (control) and the other, 15 ppm of Cd in the drinking water for 60 days (6 animal in each experimental condition, 3 independent experiments).Facultad de Ciencias Médica

Quality control of plant mixtures commercialized as sedatives in Bahia Blanca, Argentina

El consumo de plantas con fines medicinales, práctica tradicional desde tiempos inmemorables, se ha incrementado en los últimos años con respecto a anteriores. Esta práctica no parece acompañarse de un incremento en el control estatal de calidad de productos que incluyen plantas medicinales. Por esta razón se realizó un análisis de rótulos e identificación de los componentes vegetales de las mezclas sedantes comercializadas en Bahía Blanca, por ser las mismas de uso ampliamente difundido. Se relevaron todas las farmacias y principales herboristerías de la ciudad, hallándose cinco marcas comerciales. Las irregularidades observadas en los rótulos son variadas. A partir del análisis macroscópico y micrográfico, se hallaron adulteraciones de algún tipo en la totalidad de las mezclas.The consumption of plants with medicinal purposes, traditional practice from immemorial times, has increased in the last years with regard to previous. This practice does not seem to be accompanied of an increase in the state quality control of products that include medicinal plants. For this reason we propose to realize an analysis of labels and identification of the vegetable components of the sedative mixtures that are commercialized in Bahía Blanca for being one of those of more spread use. There were relieved all the drugstores and principal herbalists of the city, being recognized five commercial marks. The irregularities observed in the labels are varied. From macroscopic and micrographycal analysis, adulterations of some type were found in all mixtures.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Chiral Aminophosphines as Catalysts for Enantioselective Double-Michael Indoline Syntheses

The bisphosphine-catalyzed double-Michael addition of dinucleophiles to electron-deficient acetylenes is an efficient process for the synthesis of many nitrogen-containing heterocycles. Because the resulting heterocycles contain at least one stereogenic center, this double-Michael reaction would be even more useful if an asymmetric variant of the reaction were to be developed. Aminophosphines can also facilitate the double-Michael reaction and chiral amines are more readily available in Nature and synthetically; therefore, in this study we prepared several new chiral aminophosphines. When employed in the asymmetric double-Michael reaction between <em>ortho</em>-tosylamidophenyl malonate and 3-butyn-2-one, the chiral aminophosphines produced indolines in excellent yields with moderate asymmetric induction

Inhibition of NO Production by Grindelia argentina and Isolation of Three New Cytotoxic Saponins

A bioassay-guided phytochemical analysis of the ethanolic extract of Grindelia argentina Deble & Oliveira-Deble (Asteraceae) allowed the isolation of a known flavone, hispidulin, and three new oleanane-type saponins, 3-O-β-D-xylopyranosyl-(1→3)-β-D-glucopyranosyl-2β,3β,16α,23-tetrahydroxyolean-12-en-28-oic acid 28-O-β-D-xylopyranosyl-(1→2)-β-D-apiofuranosyl-(1→3)-β-D-xylopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl ester (2), 3-O-β-D-glucopyranosyl-2β,3β,23-trihydroxyolean-12-en-28-oic acid 28-O-β-D-xylopyranosyl-(1→2)-β-D-apiofuranosyl-(1→3)-β-D-xylopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl ester, (3) and 3-O-β-D-xylopyranosyl-(1→3)-β-D-glucopyranosyl-2β,3β,23-trihydroxyolean-12-en-28-oic acid 28-O-β-D-xylopyranosyl-(1→2)-β-D-apiofuranosyl-(1→3)-β-D-xylopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl ester (4), named grindeliosides A–C, respectively. Their structures were determined by extensive 1D- and 2D-NMR experiments along with mass spectrometry and chemical evidence. The isolated compounds were evaluated for their inhibitory activities against LPS/IFN-γ-induced NO production in RAW 264.7 macrophages and for their cytotoxic activities against the human leukemic cell line CCRF-CEM and MRC-5 lung fibroblasts. Hispidulin markedly reduced LPS/IFN-γ-induced NO production (IC50 51.4 μM), while grindeliosides A–C were found to be cytotoxic, with grindelioside C being the most active against both CCRF-CEM (IC50 4.2±0.1 μM) and MRC-5 (IC50 4.5±0.1 μM) cell lines.Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Pferschy Wenzig, Eva Maria. University of Graz; AustriaFil: Ortmann, Sabine. University of Graz; AustriaFil: Kretschmer, Nadine. University of Graz; AustriaFil: Kunert, Olaf. University of Graz; AustriaFil: Rechberger, Gerald N.. University of Graz; AustriaFil: Bauer, Rudolf. University of Graz; AustriaFil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentin