1 research outputs found
AGT haplotype in ITGA4 gene is related to antibody-mediated rejection in heart transplant patients
[Abstract] Introduction.
One of the main problems involved in heart transplantation (HT) is antibody-mediated rejection
(AMR). Many aspects of AMR are still unresolved, including its etiology, diagnosis and
treatment. In this project, we hypothesize that variants in genes involved in B-cell biology in
HT patients can yield diagnostic and prognostic information about AMR.
Methods.
Genetic variants in 61 genes related to B-cell biology were analyzed by next generation
sequencing in 46 HT patients, 23 with and 23 without AMR.
Results.
We identified 3 single nucleotide polymorphisms in ITGA4 gene (c.1845G>A, c.2633A>G,
and c.2883C>T) that conformed the haplotype AGT-ITGA4. This haplotype is associated
with the development of AMR. Moreover, AMR patients with the haplotype AGT-ITGA4
present lower levels of integrin α-4 in serum samples compared to the reference GAC haplotype
in control patients.
Conclusion.
We can conclude that polymorphisms in genes related to the biology of B-cells could have
an important role in the development of AMR. In fact, the AGT haplotype in ITGA4 gene
could potentially increase the risk of AMR.Instituto de Salud Carlos III; PI13/0217