Serpa PDO cheese: towards identification of chemical markers involved in organoleptic attributes

info:eu-repo/semantics/publishedVersio

A cultura do sorgo em sistemas integrados lavoura-pecuária ou lavoura-pecuária floresta

bitstream/item/64078/1/circ-172-1.pd

Serpa PDO cheese: towards identification of chemical markers involved in organoleptic attributes

Serpa is a PDO cheese manufactured using raw ovine milk and extracts of C. cardunculus L. as rennet, without addition of starter cultures and followed by a minimum (but safe) ripening period. Both the processing technology and ingredients used result in a high microbial biodiversity that allows the development of a unique flavor. Variations in the manufacture process and distinct milk composition among producers result in a considerably heterogeneous cheese. The present study aimed at screening some groups of sensorial related compounds during two consecutive months of production, towards the identification of chemical markers involved in the specificity of Serpa cheese. The results suggested a high diversity and heterogenous chemical composition according to the producer and month. The free amino acids (FAAs) profile suggested the presence of almost all amino acids in the analyzed cheeses, being glutamic acid, alanine, leucine, valine and phenylalanine the most prevalent ones. Regarding the organic acid profile, lactic and acetic acids were the dominant groups. The volatile analysis suggested a high diversity and variability of volatile composition between cheeses, including several chemical groups, namely, ethyl esters, aldehydes and alcohols. The identification of sensorial chemical markers will be crucial to guide the selection and development of an autochthonous starter culture to improve Serpa’s quality and safety..info:eu-repo/semantics/publishedVersio

Localization Of Metastasis Within The Sentinel Lymph Node Biopsies: A Predictor Of Additional Axillary Spread Of Breast Cancer? [localização Da Metástase No Interior Dos Linfonodos Sentinelas: Um Preditor De Disseminação Adicional Axilar Em Câncer De Mama?]

Purpose: To explore the relationship between morphological characteristics and histologic localization of metastasis within sentinel lymph nodes (SLN) and axillary spread in women with breast cancer. Methods: We selected 119 patients with positive SLN submitted to complete axillary lymph node dissection from July 2002 to March 2007. We retrieved the age of patients and the primary tumor size. In the primary tumor, we evaluated histologic and nuclear grade, and peritumoral vascular invasion (PVI). In SLNs we evaluated the size of metastasis, their localization in the lymph node, number of foci, number of involved lymph nodes, and extranodal extension. Results: Fifty-one (42.8%) patients had confirmed additional metastasis in non-sentinel lymph nodes (NLSN). High histologic grade, PVI, intraparenchymatous metastasis, extranodal neoplastic extension and size of metastasis were associated with positive NLSN. SLN metastasis affecting the capsule were associated to low risk incidence of additional metastasis. After multivariate analysis, PVI and metastasis size in the SLN remained as the most important risk factors for additional metastasis. Conclusions: The risk of additional involvement of NSLN is higher in patients with PVI and it increases progressively according the histologic localization in the lymph node, from capsule, where the afferent lymphatic channel arrives, to the opposite side of capsule promoting the extranodal extension. Size of metastasis greater than 6.0 mm presents higher risk of additional lymph node metastasis.3511483489Giuliano, A.E., Mapping a pathway for axillary staging: A personal perspective on the current status of sentinel lymph node dissection for breast cancer (1999) Arch Surg., 134 (2), pp. 195-199Ernst, M.F., Voogd, A.C., Balder, W., Klinkenbijl, J.H., Roukema, J.A., Early and late morbidity associated with axillary levels I-III dissection in breast cancer (2002) J Surg Oncol., 79 (3), pp. 151-155Edge, S.B., Niland, J.C., Bookman, M.A., Theriault, R.L., Ottesen, R., Lepisto, E., Emergence of sentinel node biopsy in breast cancer as standard-of-care in academic comprehensive cancer centers (2003) J Natl Cancer Inst., 95 (20), pp. 1514-1521Noguchi, M., Sentinel lymph node biopsy as an alternative to routine axillary lymph node dissection in breast cancer patients (2001) J Surg Oncol., 76 (2), pp. 144-156Veronesi, U., Paganelli, G., Viale, G., Luini, A., Zurrida, S., Galimberti, V., A randomized comparison of sentinel-node biopsy with routine axillary dissection in breast cancer (2003) N Engl J Med., 349 (6), pp. 546-553Fisher, B., Anderson, S., Bryant, J., Margolese, R.G., Deutsch, M., Fisher, E.R., Twenty-year follow-up of a randomized trial comparing total mastectomy, lumpectomy, and lumpectomy plus irradiation for the treatment of invasive breast cancer (2002) N Engl J Med., 347 (16), pp. 1233-1241Giuliano, A.E., Hunt, K.K., Ballman, K.V., Beitsch, P.D., Whitworth, P.W., Blumencranz, P.W., Axillary dissection vs no axillary dissection in women with invasive breast cancer and sentinel node metastasis: A randomized clinical trial (2011) JAMA., 305 (6), pp. 569-575Giuliano, A.E., Kirgan, D.M., Guenther, J.M., Morton, D.L., Lymphatic mapping and sentinel lymphadenectomy for breast cancer (1994) Ann Surg., 220 (3), pp. 391-401Czerniecki, B.J., Scheff, A.M., Callans, L.S., Spitz, F.R., Bedrosian, I., Conant, E.F., Immunohistochemistry with pancytokeratins improves the sensitivity of sentinel lymph node biopsy in patients with breast carcinoma (1999) Cancer., 85 (5), pp. 1098-1103Albertini, J.J., Lyman, G.H., Cox, C., Yeatman, T., Balducci, L., Ku, N., Lymphatic mapping and sentinel node biopsy in the patient with breast cancer (1996) JAMA., 276 (22), pp. 1818-1822Veronesi, U., Paganelli, G., Galimberti, V., Viale, G., Zurrida, S., Bedoni, M., Sentinel-node biopsy to avoid axillary dissection in breast cancer with clinically negative lymph-nodes (1997) Lancet., 349 (9069), pp. 1864-1867Kamath, V.J., Giuliano, R., Dauway, E.L., Cantor, A., Berman, C., Ku, N.N., Characteristics of the sentinel lymph node in breast cancer predict further involvement of higher-echelon nodes in the axilla: A study to evaluate the need for complete axillary lymph node dissection (2001) Arch Surg., 136 (6), pp. 688-692Veronesi, U., Paganelli, G., Viale, G., Galimberti, V., Luini, A., Zurrida, S., Sentinel lymph node biopsy and axillary dissection in breast cancer: Results in a large series (1999) J Natl Cancer Inst., 91 (4), pp. 368-373Van Zee, K.J., Manasseh, D.M., Bevilacqua, J.L., Boolbol, S.K., Fey, J.V., Tan, L.K., A nomogram for predicting the likelihood of additional nodal metastases in breast cancer patients with a positive sentinel node biopsy (2003) Ann Surg Oncol., 10 (10), pp. 1140-1151Cserni, G., Boross, G., Maráz, R., Leidenius, M.H., Meretoja, T.J., Heikkila, P.S., Multicentre validation of different predictive tools of non-sentinel lymph node involvement in breast cancer (2012) Surg Oncol., 21 (2), pp. 59-65(2010) AJCC Cancer Staging Manual, , American Joint Committee on Cancer. 7th ed. New York: Springer VerlagElston, C.W., Ellis, I.O., Pathological prognostic factors in breast cancer. I. The value of histological grade in breast cancer: Experience from a large study with long-term follow-up (1991) Histopathology., 19 (5), pp. 403-410Carcoforo, P., Maestroni, U., Querzoli, P., Lanzara, S., Maravegias, K., Feggi, L., Primary breast cancer features can predict additional lymph node involvement in patients with sentinel node micrometastases (2006) World J Surg., 30 (9), pp. 1653-1657Fougo, J.L., Afonso, M., Senhorães Senra, F., Dias, T., Leal, C., Araújo, C., Predictive factors for non-sentinel lymph node involvement in breast cancer patients with a positive sentinel node: Should we consider sentinel node-related factors? (2009) Clin Transl Oncol., 11 (3), pp. 165-171Hwang, R.F., Krishnamurthy, S., Hunt, K.K., Mirza, N., Ames, F.C., Feig, B., Clinicopathologic factors predicting involvement of nonsentinel axillary nodes in women with breast cancer (2003) Ann Surg Oncol., 10 (3), pp. 248-254Mustać, E., Matusan-Ilijas, K., Marijić, B., Smokvina, M., Jonjić, N., Predicting the likelihood of additional nodal metastases in breast carcinoma patients with positive sentinel node biopsy (2010) Int J Surg Pathol., 18 (1), pp. 36-41Viale, G., Maiorano, E., Pruneri, G., Mastropasqua, M.G., Valentini, S., Galimberti, V., Predicting the risk for additional axillary metastases in patients with breast carcinoma and positive sentinel lymph node biopsy (2005) Ann Surg., 241 (2), pp. 319-325Bolster, M.J., Peer, P.G., Bult, P., Thunnissen, F.B., Schapers, R.F., Meijer, J.W., Risk factors for non-sentinel lymph node metastases in patients with breast cancer. The outcome of a multi-institutional study (2007) Ann Surg Oncol., 14 (1), pp. 181-189Bernardi, S., Bertozzi, S., Londero, A.P., Giacomuzzi, F., Angione, V., Dri, C., Nine years of experience with the sentinel lymph node biopsy in a single Italian center: A retrospective analysis of 1,050 cases (2012) World J Surg., 36 (4), pp. 714-722Abdessalam, S.F., Zervos, E.E., Prasad, M., Farrar, W.B., Yee, L.D., Walker, M.J., Predictors of positive axillary lymph nodes after sentinel lymph node biopsy in breast cancer (2001) Am J Surg., 182 (4), pp. 316-320Chu, K.U., Turner, R.R., Hansen, N.M., Brennan, M.B., Bilchik, A., Giuliano, A.E., Do all patients with sentinel node metastasis from breast carcinoma need complete axillary node dissection? (1999) Ann Surg., 229 (4), pp. 536-541Fleming, F.J., Kavanagh, D., Crotty, T.B., Quinn, C.M., McDermott, E.W., O'Higgins, N., Factors affecting metastases to non-sentinel lymph nodes in breast cancer (2004) J Clin Pathol., 57 (1), pp. 73-76Joseph, K.A., El-Tamer, M., Komenaka, I., Troxel, A., Ditkoff, B.A., Schnabel, F., Predictors of nonsentinel node metastasis in patients with breast cancer after sentinel node metastasis (2004) Arch Surg., 139 (6), pp. 648-651Wong, S.L., Edwards, M.J., Chao, C., Tuttle, T.M., Noyes, R.D., Woo, C., Predicting the status of the nonsentinel axillary nodes: A multicenter study (2001) Arch Surg., 136 (5), pp. 563-568Zavagno, G., De Salvo, G.L., Bozza, F., Scalco, G., Marconato, R., Valletta, S., Number of metastatic sentinel nodes as predictor of axillary involvement in patients with breast cancer (2004) Breast Cancer Res Treat., 86 (2), pp. 171-179Meretoja, T.J., Vironen, J.H., Heikkilä, P.S., Leidenius, M.H., Outcome of selected breast cancer patients with micrometastasis or isolated tumor cells in sentinel node biopsy and no completion axillary lymph node dissection (2010) J Surg Oncol., 102 (3), pp. 215-219Ozcinar, B., Muslumanoglu, M., Igci, A., Gurdal, S.O., Yavuz, E., Kecer, M., Clinical importance of micrometastasis in sentinel lymph nodes (2011) Breast., 20 (1), pp. 31-33Turner, R.R., Chu, K.U., Qi, K., Botnick, L.E., Hansen, N.M., Glass, E.C., Pathologic features associated with nonsentinel lymph node metastases in patients with metastatic breast carcinoma in a sentinel lymph node (2000) Cancer., 89 (3), pp. 574-581Sachdev, U., Murphy, K., Derzie, A., Jaffer, S., Bleiweiss, I.J., Brower, S., Predictors of nonsentinel lymph node metastasis in breast cancer patients (2002) Am J Surg., 183 (3), pp. 213-217Reynolds, C., Mick, R., Donohue, J.H., Grant, C.S., Farley, D.R., Callans, L.S., Sentinel lymph node biopsy with metastasis: Can axillary dissection be avoided in some patients with breast cancer? (1999) J Clin Oncol., 17 (6), pp. 1720-1726Diaz, L.K., Hunt, K., Ames, F., Meric, F., Kuerer, H., Babiera, G., Histologic localization of sentinel lymph node metastases in breast cancer (2003) Am J Surg Pathol., 27 (3), pp. 385-389Paish, E.C., Green, A.R., Rakha, E.A., McMillan, R.D., Maddison, J.R., Ellis, I.O., Three-dimensional reconstruction of sentinel lymph nodes with metastatic breast cancer indicates three distinct patterns of tumour growth (2009) J Clin Pathol., 62 (7), pp. 617-62

Adubação nitrogenada e consorciação do milho com brachiaria ruziziensis em sistema plantio direto.

A produtividade do milho, bem como da forragem, quando em consórcio, pode ser influenciada pela adubação nitrogenada, bem como pelo arranjo entre as plantas. Assim, esse trabalho objetivou avaliar o efeito da adubação nitrogenada e consorciação do milho com Brachiaria ruziziensis sobre aspectos produtivos dessas culturas, em sistema plantio direto. Utilizou-se o delineamento experimental de blocos ao acaso, com os tratamentos distribuídos em parcelas subdivididas, com quatro repetições. Foram testadas três modalidades de cultivo (milho solteiro, consorciado com braquiária na entrelinha e consorciado com braquiária na linha + entrelinha do milho) nas parcelas e cinco doses de N total (0, 50, 100, 150, 200 kg ha-1) nas subparcelas. Avaliaram-se o rendimento de grãos e teores de N, P e K nas folhas do milho e a matéria seca de parte aérea da braquiária. A adubação nitrogenada aumentou linearmente a produtividade do milho no cultivo solteiro, bem como aumentou os teores foliares de N. Embora a análise de variância não tenha mostrado efeito significativo, os dados dos teores foliares de K apresentaram ajuste quadrático com as doses de N, ano nível de 5% de probabilidade. Em relação às modalidades de cultivo, os teores foliares de N e P foram maiores na consorciação do milho com braquiária na linha + entrelinha, em relação às duas outras modalidades, que não diferiram entre si. A matéria seca de braquiária não sofreu influência das doses de N, sendo significativamente maior no cultivo na linha + entrelinha do milho

Avaliação da imunogenicidade e proteção de vacinas recombinantes contra Corynebacterium pseudotuberculosis em camundongos.

Linfadenite caseosa é uma doença infectocontagiosa, causada pela bactéria Gram-positiva Corynebacterium pseudotuberculosis, que acomete principalmente ovinos e caprinos. Há necessidade do desenvolvimento de novas vacinas mais eficientes contra C. pseudotuberculosis, entre estas, as vacinas de DNA e de proteínas recombinantes. Isto tem sido favorecido pelos estudos sobre o genoma desta bactéria e seus determinantes moleculares. Devido à magnitude da resposta imune das vacinas de DNA ser relativamente fraca, nanopartículas preparadas por biomateriais têm sido utilizadas com o intuito de aumentar a resposta imune induzidas por estas vacinas. O gene psec foi identificado por imunovarredura de expressão gênica de C. pseudotuberculosis, e codifica uma proteína de membrana, que está envolvida na fisiologia da bactéria e invasão de células hospedeiras

Arranjo de plantas de braquiária em consórcio com a cultura do milho.

bitstream/item/111171/1/circ-202.pd