The roles of poly(ADP-ribose)-metabolizing enzymes in alkylation-induced cell death

Poly(ADP-ribose) (PAR) has been identified as a DNA damage-inducible cell death signal upstream of apoptosis-inducing factor (AIF). PAR causes the translocation of AIF from mitochondria to the nucleus and triggers cell death. In living cells, PAR molecules are subject to dynamic changes pending on internal and external stress factors. Using RNA interference (RNAi), we determined the roles of poly(ADP-ribose) polymerases-1 and -2 (PARP-1, PARP-2) and poly(ADP-ribose) glycohydrolase (PARG), the key enzymes configuring PAR molecules, in cell death induced by an alkylating agent. We found that PARP-1, but not PARP-2 and PARG, contributed to alkylation-induced cell death. Likewise, AIF translocation was only affected by PARP-1. PARP-1 seems to play a major role configuring PAR as a death signal involving AIF translocation regardless of the death pathway involved

Slowly cooling white dwarfs in M13 from stable hydrogen burning

White dwarfs (WDs) are the final evolutionary product of the vast majority of stars in the Universe. They are electron-degenerate structures characterized by no stable thermonuclear activity, and their evolution is generally described as a pure cooling process. Their cooling rate is adopted as cosmic chronometer to constrain the age of several Galactic populations, including the disk, globular and open clusters. By analysing high-resolution photometric data of two very similar Galactic globular clusters (M3 and M13), we find a clear-cut and unexpected overabundance of bright WDs in M13. Theoretical models suggest that, consistent with the horizontal branch morphology, this overabundance is due to a slowing down of the cooling process in ~70% of the WDs in M13, caused by stable thermonuclear burning in their residual hydrogen-rich envelope. The presented observational evidence of quiescent thermonuclear activity occurring in cooling WDs brings new attention on the use of the WD cooling rate as cosmic chronometer for low-metallicity environments

Formation and evolution of a 0.242 Msun helium white dwarf in presence of element diffusion

A 0.242 Msun object that finally becomes a helium white dwarf is evolved from Roche lobe detachment down to very low luminosities. In doing so, we employ our stellar code to which we have added a set of routines that compute the effects due to gravitational settling, and chemical and thermal diffusion. Initial model is constructed by abstracting mass to a 1 Msun red giant branch model up to the moment at which the model begins to evolve bluewards. We find that element diffusion introduces noticeable changes in the internal structure of the star. In particular, models undergo three thermonuclear flashes instead of one flash as we found with the standard treatment. This fact has a large impact on the total mass fraction of hydrogen left in the star at entering the final cooling track. As a result, at late stages of evolution models with diffusion are characterized by a much smaller nuclear energy release, and they evolve significantly faster compared to those found with the standard treatment. We find that models in which diffusion is considered predict evolutionary ages for the white dwarf companion to the millisecond pulsar PSR B1855+09 in good agreement with the spin-down age of the pulsar.Comment: 6 pages, 3 figures, 12th European Workshop on White Dwarf

Labels direct infants’ attention to commonalities during novel category learning

Recent studies have provided evidence that labeling can influence the outcome of infants’ visual categorization. However, what exactly happens during learning remains unclear. Using eye-tracking, we examined infants’ attention to object parts during learning. Our analysis of looking behaviors during learning provide insights going beyond merely observing the learning outcome. Both labeling and non-labeling phrases facilitated category formation in 12-month-olds but not 8-month-olds (Experiment 1). Non-linguistic sounds did not produce this effect (Experiment 2). Detailed analyses of infants’ looking patterns during learning revealed that only infants who heard labels exhibited a rapid focus on the object part successive exemplars had in common. Although other linguistic stimuli may also be beneficial for learning, it is therefore concluded that labels have a unique impact on categorization

Effect of biochar on the mirobial enzymes activity of soil with Eucalyptus.

Session 5- Bacterial and Plant Physiology - Poster V.5