IN VITRO INVESTIGATION OF ANTIBACTERIAL ACTIVITY OF DRUGS USED IN SEDATION IN INTENSIVE CARE UNIT

WOS: 000496261400099Introduction: Sepsis is a systemic inflammatory response to infection and is a life-threatening condition. Sedation is important such as these patients. Some drugs that used for sedation may have antimicrobial effects. the aim of this study was to investigate the antimicrobial activity of propofol, ketamine, thiopental, dexmedotomidine and midazolam, which are widely used in intensive care in the form of infusion for sedation. Materials and methods: in vitro antimicrobial activities of dexmedetomidine, midazolam, ketamine, propofol and thiopental sodyom drugs of different concentrations were investigated by using the agar well diffusion method. Ampicillin, streptomycin and fluconazole were used as standard antibacterial and antifungal drugs, respectively. While selecting the microorganisma, the agents which are unexpected to cause sepsis were included in the study in addition to the causative agents leading to sepsis, in order to understand the antimicrobial effect more clearly. All test microorganisms as follows: E. coli ATCC, Y. pseudotuberculosis ATCC, P. auroginosa ATCC, E. faecalis ATCC, S. aureus ATCC, B. cereus, M. smegmatis ATCC, C. albicans ATCC, C. tropicalis ATCC and S. cerevisiae ATCC. Results: Ketamine was found to have antimicrobial activity against all of the microorganisms tested, but the highest efficacy was determined against S. aureus. Bactericidal (and fungocidal) activity of ketamine was determined against all other microorganisms. Conclusion: We consider that it can be important to conduct the trial on the doses used in practice, and to demonstrate the usefulness of the antibacterial activity of these drugs in practice. in conclusion, in patients with sepsis followed in the intensive care unit, ketamine may be preferred for sedation. Thus, this can contribute to the patient's antibiotherapy. However, further clinical research is needed to evaluate the effectiveness of antimicrobial activity of ketamine in clinical practice and to assess the effectiveness of reducing the mortality in patients with sepsis

Synthesis of Novel Antipyrine Derivatives Possessing Remarkable Antimicrobial Activities

WOS: 000498981700012In this study, new triazole, thiazole, oxazole and Mannich bases were synthesized starting from antipyrine. All the newly synthesized compounds were screened for their antimicrobial activities against some test microorganisms and among them most of them show very good antituberculosis activity beside gram positive and gram negative bacteria. the structures of all synthesized compounds were determined by spectroscopic methods such as FT-IR, H-1 NMR and C-13 NMR.Scientific research project of Karadeniz Technical University (KTU-BAP) [5289]This work was supported by the Scientific research project of Karadeniz Technical University (KTU-BAP) [grant numbers 5289]

Antimicrobial Activity and Composition of Rindera lanata (LAM.) Bunge var. canescens (ADC) Kosn. Essential oil Obtained by Hydrodistillation and Microwave Assisted Distillation

YUCEL, TAYYIBE BEYZA/0000-0002-2632-8325WOS: 000403499200013The composition of essential oils of Rindera lanata (LAM). Bunge var. canescens (A.D.C) Kosn. obtained by hydro-distillation (HD) and microwave assisted distillation (MW) by GC, GC/MS. Thirty three and thirty nine compounds were identified in the oils representing 81.20% and 78.57% of the oils obtained by HD and MW respectively. Aldehydes were shown to be the main group of constituents of the MW 40.11% and 15.23%, respectively. However, the major group in the HD was found to be 25.35% alcohols and 23.78% hydrocarbons. 6-Methyl heptan-2-ol (15.97%) was the main compound of the HD. Furthermore, in the MW assisted essential oil, the major compound present was decane (10.50%). Terpenoid class compounds were found in essential oils and oxygenated monoterpenes were determined as major group (13.34% and 7.19%). Antimicrobial activity of the isolated essential oils of the plant was also investigated and they showed moderate antimicrobial activity against the tested microorganisms.Karadeniz Technical University Research Fund; State Planning Agency (DPT) of TurkeyTurkiye Cumhuriyeti Kalkinma BakanligiThis work was supported by grants from Karadeniz Technical University Research Fund and State Planning Agency (DPT) of Turkey

Antimicrobial Activity and Composition of Rindera lanata (LAM.) Bunge var. canescens (ADC) Kosn. Essential oil Obtained by Hydrodistillation and Microwave Assisted Distillation

YUCEL, TAYYIBE BEYZA/0000-0002-2632-8325WOS: 000403499200013The composition of essential oils of Rindera lanata (LAM). Bunge var. canescens (A.D.C) Kosn. obtained by hydro-distillation (HD) and microwave assisted distillation (MW) by GC, GC/MS. Thirty three and thirty nine compounds were identified in the oils representing 81.20% and 78.57% of the oils obtained by HD and MW respectively. Aldehydes were shown to be the main group of constituents of the MW 40.11% and 15.23%, respectively. However, the major group in the HD was found to be 25.35% alcohols and 23.78% hydrocarbons. 6-Methyl heptan-2-ol (15.97%) was the main compound of the HD. Furthermore, in the MW assisted essential oil, the major compound present was decane (10.50%). Terpenoid class compounds were found in essential oils and oxygenated monoterpenes were determined as major group (13.34% and 7.19%). Antimicrobial activity of the isolated essential oils of the plant was also investigated and they showed moderate antimicrobial activity against the tested microorganisms.Karadeniz Technical University Research Fund; State Planning Agency (DPT) of TurkeyTurkiye Cumhuriyeti Kalkinma BakanligiThis work was supported by grants from Karadeniz Technical University Research Fund and State Planning Agency (DPT) of Turkey

Antimicrobial Activity of Bacillus Strains Isolated from Spring Water and A Novel Bacteriocin: RS108

WOS: 000331428900033The aim of this research was to investigate the bacteriocins produced by Bacillus strains isolated from the spring water of Rize, in Turkey. Bacillus cereus RS108 was identified by both conventional and molecular methods, bacteriocin RS108 which was produced by RS108, was partially characterized. A broad range of indicator strains, including several species of bacteria and yeast like fungi, was inhibited by a crude bacteriocin obtained from culture supernatant fluid. The best antimicrobial activity of RS108 was detected on S. pyogenes, L. monocitogenes, and another B. cereus strains, and at the late exponential growth phase. RS108 was stable at 90 degrees C, but the activity was lost when the temperature reached 100 degrees C. It was inactivated by proteinase K. It was resistant 10% ration of some solvent (chloroform, ethanol etc.), but sensitive high concentration. Bacteriocin activity was observed in the pH range of 3.0-9.0. SDS-PAGE analysis of the partially purified bacteriocin shows that the molecular weight of bacteriocin RS108 is approximately 4 kDa

Synthesis and biological activity studies of new hybrid molecules containing tryptamine moiety

WOS: 000298882100010The synthesis of N'-(4-substitutedphenylsulfonyl)-2-{4-[2-(1H-indol-yl)ethyl]-3-(4-chlorobenzyl)-5-oxo-4,5-dihydro-1H-1,2,4-triazol-1-yl}acetohydrazides (3a-c), 2-{4-[2-(1H-indol-3-yl)ethyl]-3-(4-chlorobenzyl)-5-oxo-4,5-dihydro-1H-1,2,4-triazol-1-yl}-N'-aryl methylidene acetohydrazides (4a-f) and 4-[2-(1H-indol-3-yl)ethyl]-5-(4-substitutedbenzyl)-2-[(5-sulfanyl-1,3,4-oxadiazol-2-yl)methyl]-2,4-dihydro-3H-1,2,4-triazol-3-ones (5a, b) was performed starting from the corresponding acid hydrazides (2a, b) which was reported earlier. The treatment of 1,3,4-oxadiazole derivatives (5a, b) with hydrazine hydrate produced 4-amino-5-sulfanyl-4H-1,2,4-triazol-3-yl derivatives (6a, b). Then, compound 6b was converted to the corresponding Schiff base (7) by the treatment with anisaldehyde. The synthesis of 5-(4-chlorobenzyl)-4-[2-(1H-indol-3-yl)ethyl]-2-[(4-benzyl-5-oxo-4,5-dihydro-1H-1,2,4-triazol-3-yl)methyl]-2,4-dihydro-3H-1,2,4-triazol-3-one (8) and 5-(4-methylbenzyl)-4-[2-(1H-indol-3-yl)ethyl]-2-[(4-benzyl-5-oxo-4,5-dihydro-1H-1,2,4-triazol-3-yl)methyl]-2,4-dihydro-3H-1,2,4-triazol-3-one (10) was carried out by the reaction of acid hydrazides (2a, b) with aryl iso(thio)cyanates either via the formation of the intermediates (9a, b) (for 10) or direct cyclization (for 8). 1,3-Oxa(thia)zol-2(3H)-ylidene]acetohydrazide derivatives (11a, b) were obtained by the reaction of 9a, b with 4-chlorophenacyl bromide. All newly synthesized compounds were screened for their antimicrobial activities and some of which was found to be active against the test microorganisms.Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [107T333]; Karadeniz Technical University, BAP, TurkeyKaradeniz Teknik University [2007.111.002.6]This project was supported by Scientific and Technological Research Council of Turkey (TUBITAK, Project no: 107T333) and Karadeniz Technical University, BAP, Turkey (Ref. No. 2007.111.002.6) and is gratefully acknowledged

SOME AZO DYES CONTAINING EUGENOL AND GUAIACOL, SYNTHESIS, ANTIOXIDANT CAPACITY, UREASE INHIBITORY PROPERTIES AND ANTI-HELICOBACTER PYLORI ACTIVITY

Sasmaz, Selami/0000-0001-5311-7550; kantar, cihan/0000-0002-5234-0782WOS: 000442387600004There are many studies about azo compounds at literature. However, antioxidant capacity, urease inhibitory and anti-Helicobacter pylori activity properties are not well known. Therefore, some azo dyes containing eugenol and guaiacol were synthesized by coupling reactions of different amines with eugenol and guaiacol, respectively. the newly synthesized compounds were screened for their antioxidant properties by employing three in vitro assays like 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay and CUPric Reducing Antioxidant Capacity (CUPRAC). All compounds were assayed for inhibitory effect against urease and Helicobacter pylori growth in vitro. These results suggest that compounds 1, 5, 6 and 7 can be novel urease inhibitory and anti-Helicobacter pylori agent.Recep Tayyip Erdogan University Scientific Research Project Units [2015.53007.102.02.02]This study was supported by the Recep Tayyip Erdogan University Scientific Research Project Units (Project No: 2015.53007.102.02.02)

Trichoderma lixii ID11D seed biopriming mitigates dose dependent salt toxicity in maize

PEHLIVAN, NECLA/0000-0002-2045-8380WOS: 000396112600014Certain number of commercial products has been developed to grow stress resistant plants for the sustainability of agriculture in current era with full of energy dependency and hunger besides global warming. Given the risk of these products on the microbial environment in the rhizosphere and food security of mankind due to the accumulated chemical pollution through the food web, organic fungal plant growth promoting agents might be used. Hence, effects of Trichoderma lixii ID11D (TXD), our local fungal isolate on Zea mays L. cv. Samada 07 as a seed biopriming agent was investigated under dose dependent NaCl stress in this study. Fungus was characterized based on ITS (internal transcribed spacer) regions via clustering approach after isolated from tea plantation area. the higher the NaCl concentration, the more effective the TXD was observed. Improved electron transport rate (ETR), maximum quantum efficiency of PS II (Fv/Fm), the effective quantum yield of PS2 (Phi PS2), photochemical quenching (qP) and decreased non-photochemical quenching (NPQ) was detected in TXD primed plants after NaCl expose. TXD seed biopriming increased the lengths, fresh and dry weights of root/shoots and decreased the lipid peroxidation (MDA) remarkably. Non-enzymatic pathway was found to be more effective than enzymatic one for seed bioprimed plants proven by higher RWC, soluble protein, proline, chlorophyll, carotenoid, and less H2O2 and MDA levels under each dose of NaCl. Detected phenotypic and biochemical improvements pave the way of the potential usage of the formulated fungus biopreperations as a pre-harvest agent in agriculturally important cereals in the future and going beyond lab-based level.Recep Tayyip Erdogan University Research FundRecep Tayyip Erdogan University [2012.102.03.3, 2015.53001.102.03.07]This work was financed by the Recep Tayyip Erdogan University Research Fund (Project Numbers: 2012.102.03.3 and 2015.53001.102.03.07). We thank PhD candidate Sule Guzel for her valuable help

Salt stress resilience potential of a fungal inoculant isolated from tea cultivation area in maize

PEHLIVAN, NECLA/0000-0002-2045-8380WOS: 000405518200005Agriculture needs to be sustained by organic processes in current era as population explosion energy and the number of individuals undernourished are raising public concerns. Global warming poses additional threat by lifting the damage of salt stress especially in agro-economically vital crops like maize whose cultivation dates back to Mayans. To that end, cost-effective and organic fungal agents may be great candidates in stress resilience. We isolated the fungal strain from the soil of tea plants and characterized that via 5.8 S rDNA gene with internal transcribed spacer ITS-1 and ITS-2 regions, then named the target strain as TA. Reduced maximum quantum efficiency of PS II (Fv/Fm), the effective quantum yield of PS2 (Phi FPS2), electron transport rate (ETR), photochemical quenching (qP) and increased non-photochemical quenching (NPQ) were detected in maize plants stressed with dose dependent salt. Enhanced Fv/Fm, Phi FPS2, ETR, qP and decreased NPQ was observed in TA primed plus NaCl treated plants. TA biopriming significantly increased the lengths, fresh and dry weights of root/shoots and decreased the lipid peroxidation. Maize seedlings bioprimed with TA had less MDA and higher soluble protein, proline, total chlorophyll, carotenoid and RWC under NaCl. Furthermore, SOD, GPX and GR activities were much more increased in root and leaves of TA primed seedlings, however CAT activity did not significantly change. This is the first report to our knowledge that TA reverses the damage of NaCl stress on maize growth through improving water status, antioxidant machinery and especially photosynthetic capacity.Research Fund of Recep Tayyip Erdogan UniversityRecep Tayyip Erdogan University [2012.102.03.3, 2015.53001.102.03.07]This work was supported by the Research Fund of Recep Tayyip Erdogan University (project numbers: 2012.102.03.3 and 2015.53001.102.03.07)

Composition of Volatile Components and Biological Activities of Aethionema diastrophis

EYUPOGLU, Ozan Emre/0000-0002-4449-0537WOS: 000400195700044[No abstract available]KTU Research FundKaradeniz Technical University [5581]This study was supported by the KTU Research Fund (Project No. 5581)