Dark Charades

It is my belief that every creative act must stand or fall on its intrinsic merits without recourse to props or other would-be aids that anyone of authority or otherwise, might attempt to supply. After the fact of creation the only legitimate manner by which the author may attempt to strengthen his work is by revision. He may, if he so desires, use footnotes or appendices or introductions to explain what he is attempting to do or say in his work. He has no right to attempt to force the reader to accept or agree with what the author hopes he has said in his work. The poem or story must speak for itself. I make no claims that the poems in this volume are great works of art; I do, however, claim that if they make any pretense to being worth reading, this claim must be supported by the poems themselves and not by any word I may write in this introduction. The poems must stand or fall solely on their intrinsic merit. If what they have to say is not worth saying, or is said in a dull or unintelligible manner, let them be forgotten

A systematic approach to project portfolio selection for economic development in municipalities: a case study in Vienna, Missouri

Economic development is essential for the survival of all municipalities in the United States. Economic growth, created through project implementation, brings new capital into municipalities by increasing the tax base. Smaller municipalities often struggle to implement projects that promote economic growth. These municipalities run into problems because they lack resources to hire an economic consultant and capabilities to independently complete the strategic planning process. This thesis describes an easy-to-use and easy-to-implement systematic ten-step approach to project portfolio selection for economic growth within smaller municipalities...The ten-step approach was employed in a case study performed in Vienna, Missouri. The case study involved eight taxpayer-owned projects ranging in budget from three hundred dollars to over one-million dollars. A group of sixteen community members were involved in the application of the ten-step approach. By following the systematic approach, these community members developed a project portfolio for the City of Vienna aimed at promoting economic development

X-Ray Microanalysis of Urinary Stones, a Comparison with Other Methods

A previous study of urinary stones by a combined electron microscopy demonstrated the potential of scanning electron microscopy and X- ray analysis as an analytic tool for urinary stones. Electron diffraction was chosen for the final confirmation of crystals in the study. Although electron diffraction is highly accurate for this purpose, it is desirable to establish the sensitivity of X-ray analysis for the identification of stone components relative to the more commonly used methods. Eighty six consecutive urinary stones were analyzed by X-ray analysis and the findings were compared with those of X-ray diffraction, infrared spectrometry and chemical analysis. The results indicate that X-ray analysis exceeds X-ray diffraction and infrared spectroscopy in its sensitivity for the identification of stone components several fold. This was largely due to the inability of the latter methods to detect apatite in more than half of the apatite containing stones. The findings in X-ray analysis had the best correlation with chemical analysis, which was applied mainly to the detection of apatite. X-ray analysis is particularly suited for the detection of rare and minor inorganic components of urinary stones such as silica and gypsum, and is obviously one of the most powerful tools for the analysis of urinary stones. Further application of X-ray analysis to urinary stone is likely to discern rare inorganic components of urinary stones overlooked by other methods

Les émotions collectives et le mouvement des fédérations (1789-1790)

Entre l'été 1789 et l'été 1790, le mouvement fédératif s'étend à tout le territoire français : après des débuts modestes sous la forme de rassemblements locaux en soutien aux gardes nationaux dans la région de la Loire, il rassemble rapidement des communautés et des régions entières, jusqu'à attirer des dizaines puis des centaines de milliers de participants. Cet article revient sur l'histoire du mouvement en portant une attention particulière au rôle des émotions collectives dans la mobilisation populaire. Les récits des acteurs et témoins des fêtes fédératives, que diffusent à travers le pays les brochures, feuilles volantes, journaux et la correspondance, évoquent tous la genèse d'un nouvel esprit révolutionnaire mêlant union fraternelle et solidarité contre les ennemis de la Révolution et contribuent à attirer des foules toujours plus nombreuses. Alors que l'étude des émotions s'est très souvent limitée à l'analyse de leurs expressions littéraires et de leurs effets sur les élites, ce projet élargit quant à lui le champ d'application du concept et cherche à comprendre comment ces rassemblements pleins d'émotion ont façonné la première année de la Révolution.Between the summers of 1789 and 1790, the Federation movement swept across France. What began as local National Guard rallies soon became vast gatherings, drawing tens and soon hundreds of thousands of participants. This article emphasizes the uses of collective emotion – both fraternal union and fear of enemies – in the movement, and its spread through pamphlet, newspaper and epistolary accounts. Whereas the study of emotion in the Revolution has largely been limited to literary manifestations and effects on elites, this project looks at how the emotions of large-scale gatherings helped shape the period

Medical Laboratory Scientists’ Perceptions of Their Affective Domain Development

The affective and professional development of health care professionals, including medical laboratory science (MLS) professionals, is important for their employability and provision of effective patient care. The problem is that affective development is critical to graduate employability and the quality of care provided in health care settings, yet there is limited research about which activities and experiences are perceived by MLS students as beneficial for their affective domain development. The purpose of this basic qualitative study was to understand how MLS students describe the usefulness of program learning activities and experiences as supporting their affective development, within the framework of social cognitive and emotional intelligence theories, through semistructured interviews. A total of 12 MLS program graduates from one large Midwestern university were interviewed. Data were analyzed using open manual coding to identify emergent themes. The data revealed that their affective domain development was influenced by interactions with different types of people through a variety of learning experiences and activities in diverse settings integrated during the program coursework. By integrating affective domain components into program coursework, MLS programs may provide opportunities that are supportive of student affective domain development that will be beneficial to their employability and the quality of care that they provide to patients. Developing coursework activities that enhance and are supportive of student affective development will promote positive social change by increasing graduate employability to help reduce the workforce shortage of MLS professionals, as well as promoting quality of patient care in healthcare settings

Men, Women, and Ghosts in Science

Science suffers because, by favouring the self-confident of both sexes, we discriminate against women

Molecular Plasticity of E-Cadherin and Sialyl Lewis X Expression, in Two Comparative Models of Mammary Tumorigenesis

The process of metastasis involves a series of steps and interactions between the tumor embolus and the microenvironment. Key alterations in adhesion molecules are known to dictate progression from the invasive to malignant phenotype followed by colonization at a distant site. The invasive phenotype results from the loss of expression of the E-cadherin adhesion molecule, whereas the malignant phenotype is associated with an increased expression of the carbohydrate ligand-binding epitopes, (e.g. Sialyl Lewis (x/a)) that bind endothelial E-selectin of the lymphatics and vasculature.Our study analyzed the expression of two adhesion molecules, E-cadherin and Sialyl Lewis x (sLe(x)), in both a canine mammary carcinoma and human inflammatory breast cancer (IBC) model, using double labelled immunofluorescence staining.Our results demonstrate that canine mammary carcinoma and human IBC exhibit an inversely correlated cellular expression of E-cadherin and sLe(x) within the same tumor embolus.Our results in these two comparative models (canine and human) suggest the existence of a biologically coordinated mechanism of E-cadherin and sLe(x) expression (i.e. molecular plasticity) essential for tumor establishment and metastatic progression

A production of Jean-Baptiste Molier`e's The doctor in spite of himself

The purpose of this thesis is to study the background surrounding the playwright and the play itself in preparation for a production of the play and then present a critical analysis of the production. The first chapter contains the biographical information on the playwright, the historical considerations necessary to have an understanding of the play, and the director's justification of choice of script and of his interpretation of that script. The second chapter is the director's prompt book of The Doctor in Spite of Himself, performed at The Parkway Playhouse, the University of North Carolina's summer theatre at Burnsville, North Carolina, at eight-thirty the evenings of July 12 through 15, 1972. Included are notations relevant to movement, picturization, and stage business. Floor plans and pictures provide additional material to aid in the understanding of the director's approach to the production. The final chapter consists of a critical analysis of the production. This chapter will contain three sections: (a) the director's interpretation, the style of the production, and the mood he attempted to establish} (2) the director's relationships with the actors during rehearsal and production; and (3) the audience response

EZH2 knockdown suppresses the growth and invasion of human inflammatory breast cancer cells.

INTRODUCTION: Inflammatory breast cancer (IBC) is the most metastatic variant of breast cancer with the poorest survival in all types of breast cancer patients and presently therapeutic targets for IBC are very limited. Enhancer of zeste homolog 2 (EZH2) is frequently expressed in human IBC and its expression positively correlates with worse clinical outcome. However, the molecular basis for EZH2 promoting IBC has not been explored. Here, we investigated the functional role of EZH2 in IBC cells by examining the effects of its knockdown on the formation of tumor spheroids and invasion of these cells in vitro and in vivo in an orthotopic xenograft model. METHODS: SUM149 and a new IBC cell line-FC-IBC-02 derived from pleural effusion fluid of an IBC patient were used in this study. Specific knockdown of EZH2 was performed using short hairpin RNA (shRNA) specific to the human EZH2 gene. Cell growth and the formation of tumor spheroids were examined in vitro. The effects of EZH2 knockdown on IBC cell migration and invasion were examined by a Boyden chamber assay. For the in vivo tumor growth studies, IBC cells were orthotopically transplanted into the mammary fat pads of immunodeficient mice. RESULTS: The results showed that EZH2 is expressed at higher levels in human IBC cell lines compared with normal human mammary epithelial cells, and the knockdown of EZH2 expression significantly suppressed cell growth and tumor spheroid formation of human IBC cells in vitro. In addition, EZH2 knockdown inhibited the migration and invasion of IBC cells. Significantly, EZH2 knockdown suppressed the angiogenesis and tumor growth of IBC cells in vivo. CONCLUSIONS: Our results provide direct evidence that EZH2 is critical for the formation of tumor spheroids and invasion of human IBC cells and could be a potential target for developing novel therapeutic strategies for human IBC

Gain in cellular organization of inflammatory breast cancer: A 3D in vitro model that mimics the in vivo metastasis

<p>Abstract</p> <p>Background</p> <p>The initial step of metastasis in carcinomas, often referred to as the epithelial-mesenchymal transition (EMT), occurs via the loss of adherens junctions (e.g. cadherins) by the tumor embolus. This leads to a subsequent loss of cell polarity and cellular differentiation and organization, enabling cells of the embolus to become motile and invasive. However highly malignant inflammatory breast cancer (IBC) over-expresses E-cadherin. The human xenograft model of IBC (MARY-X), like IBC, displays the signature phenotype of an exaggerated degree of lymphovascular invasion (LVI) <it>in situ </it>by tumor emboli. An intact E-cadherin/α, β-catenin axis mediates the tight, compact clump of cells found both <it>in vitro </it>and <it>in vivo </it>as spheroids and tumor emboli, respectively.</p> <p>Methods</p> <p>Using electron microscopy and focused ion beam milling to acquire <it>in situ </it>sections, we performed ultrastructural analysis of both an IBC and non-IBC, E-cadherin positive cell line to determine if retention of this adhesion molecule contributed to cellular organization.</p> <p>Results</p> <p>Here we report through ultrastructural analysis that IBC exhibits a high degree of cellular organization with polar elements such as apical/lateral positioning of E-cadherin, apical surface microvilli, and tortuous lumen-like (canalis) structures. In contrast, agarose-induced spheroids of MCF-7, a weakly invasive E-cadherin positive breast carcinoma cell line, do not exhibit ultrastructural polar features.</p> <p>Conclusions</p> <p>This study has determined that the highly metastatic IBC with an exaggerated malignant phenotype challenges conventional wisdom in that instead of displaying a loss of cellular organization, IBC acquires a highly structured architecture.</p> <p>These findings suggest that the metastatic efficiency might be linked to the formation and maintenance of these architectural features. The comparative architectural features of both the spheroid and embolus of MARY-X provide an <it>in vitro </it>model with tractable <it>in vivo </it>applications.</p