Role of Next-Generation RNA-Seq Data in Discovery and Characterization of Long Non-Coding RNA in Plants

The next-generation sequencing (NGS) technologies embrace advance sequencing technologies that can generate high-throughput RNA-seq data to delve into all the possible aspects of the transcriptome. It involves short-read sequencing approaches like 454, illumina, SOLiD and Ion Torrent, and more advance single-molecule long-read sequencing approaches including PacBio and nano-pore sequencing. Together with the help of computational approaches, these technologies are revealing the necessity of complex non-coding part of the genome, once dubbed as “junk DNA.” The ease in availability of high-throughput RNA-seq data has allowed the genome-wide identification of long non-coding RNA (lncRNA). The high-confidence lncRNAs can be filtered from the set of whole RNA-seq data using the computational pipeline. These can be categorized into intergenic, intronic, sense, antisense, and bidirectional lncRNAs with respect to their genomic localization. The transcription of lncRNAs in plants is carried out by plant-specific RNA polymerase IV and V in addition to RNA polymerase II and target the epigenetic regulation through RNA-directed DNA methylation (RdDM). lncRNAs regulate the gene expression through a variety of mechanism including target mimicry, histone modification, chromosome looping, etc. The differential expression pattern of lncRNA during developmental processes and different stress responses indicated their diverse role in plants

Learning from Past in the Commercial Air Transport Industry: A Bibliometric Analysis and Systematic Literature Review in the Safety Management Framework

In the commercial air transport industry, numerous accidents have occurred because stakeholders have failed to learn lessons from the past. The reactive hazard identification (HI) methodology in the current regulatory safety management framework provides a fresh opportunity to negotiate the challenges of the air transportation sector\u27s predicted growth and societal safety expectations. In the regulatory framework, ‘learning from the past’ is related to the reactive method of HI, and it is viewed as a data-driven decision-making process in this review. This literature review aims to investigate academic literature on ‘learning from the past’ in the aviation industry to comprehend an overview and identify the gaps in the scholarly approaches and regulatory provisions. The data sources for the review comprise the research articles indexed in Scopus and Web of Science databases from 2000 to July 2023. This review follows a mixed methodology consisting of a bibliometric analysis using an R tool to develop a broad understanding and, for specific gap identification, a systematic literature review in compliance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. All 283 studies are included in the bibliometric analysis, while only 24 studies are found eligible based on the predefined inclusion/exclusion criteria for systematic review. The results reveal that ‘learning from the past’ has not been explored as a data-driven decision-making process for HI in aviation industry settings. The stagewise barriers in the learning process and the impact of each stage on overall learning remain unexplored. The regulatory provisions associated with safety information from aviation activities\u27 resilient (positive) outcomes for learning from the past purpose, organizational safety culture, and harmonization with the criminal justice system are vulnerable

Value stream mapping to reduce the lead-time of a product development process

Product development (PD) is a broad field of endeavor dealing with the planning, design, creation, and marketing of a new product. This revolutionary research domain has become of paramount importance to beat the competition for multidisciplinary products which are larger in size and have a longer development time. The main focus of this paper is to exploit lean thinking concepts in order to manage, improve and develop the product faster while improving or at least maintaining the level of performance and quality. Lean thinking concepts encompass a board range of tools and methods intended to produce bottom line results however, value stream mapping (VSM) method is used to explore the wastes, inefficiencies, non-valued added steps in a single, definable process out of complete product development process (PDP). This single step is highly complex and occurs once while the PDP lasts for 3-5 years. A case study of gas turbine product has been discussed to illustrate and justify the use of proposed framework. In order to achieve this, the following have been performed: First of all a current state map is developed using the Gemba walk. Furthermore, Subject Matter Experts (SMEs) brainstormed to explore the wastes and their root causes found during the Gemba walk and current state mapping. A future state map is also developed with removing all the wastes/inefficiencies. Besides numerous intangible benefits, it is expected that the VSM framework will help the development teams to reduce the PD lead-time by 50%

Berberine modulates AP-1 activity to suppress HPV transcription and downstream signaling to induce growth arrest and apoptosis in cervical cancer cells

<p>Abstract</p> <p>Background-</p> <p>Specific types of high risk Human papillomaviruses (HR-HPVs) particularly, HPV types 16 and 18 cause cervical cancer and while the two recently developed vaccines against these HPV types are prophylactic in nature, therapeutic options for treatment and management of already existing HPV infection are not available as yet. Because transcription factor, Activator Protein-1 (AP-1) plays a central role in HPV-mediated cervical carcinogenesis, we explored the possibility of its therapeutic targeting by berberine, a natural alkaloid derived from a medicinal plant species, <it>Berberis </it>which has been shown to possess anti-inflammatory and anti-cancer properties with no known toxicity; however, the effect of berberine against HPV has not been elucidated.</p> <p>Results-</p> <p>We studied the effect of berberine on HPV16-positive cervical cancer cell line, SiHa and HPV18-positive cervical cancer cell line, HeLa using electrophoretic mobility gel shift assays, western and northern blotting which showed that berberine could selectively inhibit constitutively activated AP-1 in a dose- and time-dependent manner and downregulates HPV oncogenes expression. Inhibition of AP-1 was also accompanied by changes in the composition of their DNA-binding complex. Berberine specifically downregulated expression of oncogenic c-Fos which was also absent in the AP-1 binding complex. Treatment with berberine resulted in repression of E6 and E7 levels and concomitant increase in p53 and Rb expression in both cell types. Berberine also suppressed expression of telomerase protein, hTERT, which translated into growth inhibition of cervical cancer cells. Interestingly, a higher concentration of berberine was found to reduce the cell viability through mitochondria-mediated pathway and induce apoptosis by activating caspase-3.</p> <p>Conclusion-</p> <p>These results indicate that berberine can effectively target both the host and viral factors responsible for development of cervical cancer through inhibition of AP-1 and blocking viral oncoproteins E6 and E7 expression. Inhibition of AP-1 activity by berberine may be one of the mechanisms responsible for the anti-HPV effect of berberine. We propose that berberine is a potentially promising compound for the treatment of cervical cancer infected with HPV.</p

Electrical Discharge Coating a Potential Surface Engineering Technique: A State of the Art

Electrical discharge coating (EDC) process is used to deposit material on workpiece surface from sacrificial or green compact tool electrode in an electrical discharge machine. The paper presents the mechanism of EDC using green compact electrode and powder mixed dielectric methods. The tool electrode material, electrode size, process parameters, and type of dielectrics can directly affect the surface integrity of workpiece. Here, a process map of EDC as a function of process parameters, its classification, advantages, and applications for a wide range of engineering materials offers a proper template for the evaluation of coating phenomena. This study shows that EDC is an economic process as compared to other costlier techniques. Additionally, the effect of various EDM and EDC parameters on surface integrity and tribological behavior of deposited coatings is studied with their pros and cons. Finally, the current research trends of EDC and its challenges are elaborated.info:eu-repo/semantics/publishedVersio

Safety management system and hazards in the aircraft maintenance industry: a systematic literature review

In the last decade, the aircraft maintenance industry has experienced a paradigm shift in safety management. This is primarily due to the implementation of Safety Management Systems (SMS) in its business practices. The critical facet of such SMS recognizes hazards ahead of time. This review aims to undertake scholarly research to enable the identification of numerous hazards within the aircraft maintenance industry. This will be done by reviewing research articles indexed in Scopus and Web of Science databases from 2010 to September 2022. Complying with the guidelines of the PRISMA 2020 updated statement, the Systematic Literature Review (SLR) methodology is adopted for the review. The SMS-based framework was formulated to determine the inclusion and exclusion criteria, which identified 39 studies for inclusion. The key outcomes are (i) Thirty-five studies identified six hazard-prone areas and associated hazards of the aircraft maintenance industry, whereas four research studies (two each) underscored the factors impeding the safety critical SMS enactment and organizational learning from past accidents and incidents, (ii) Reviewed literature is a mix of both reactive and proactive methodologies of hazard identification (iii) Learning from past events is critical in safety management

Human Immunodeficiency Virus Type-1 (HIV-1) Transcriptional Regulation, Latency and Therapy in the Central Nervous System

The central nervous system (CNS) is highly compartmentalized and serves as a specific site of human immunodeficiency virus (HIV) infection. Therefore, an understanding of the cellular populations that are infected by HIV or that harbor latent HIV proviruses is imperative in the attempts to address cure strategies, taking into account that HIV infection and latency in the CNS may differ considerably from those in the periphery. HIV replication in the CNS is reported to persist despite prolonged combination antiretroviral therapy due to the inability of the current antiretroviral drugs to penetrate and cross the blood–brain barrier. Consequently, as a result of sustained HIV replication in the CNS even in the face of combination antiretroviral therapy, there is a high incidence of HIV-associated neurocognitive disorders (HAND). This article, therefore, provides a comprehensive review of HIV transcriptional regulation, latency, and therapy in the CNS

Human Immunodeficiency Virus Type-1 (HIV-1) Transcriptional Regulation, Latency and Therapy in the Central Nervous System

The central nervous system (CNS) is highly compartmentalized and serves as a specific site of human immunodeficiency virus (HIV) infection. Therefore, an understanding of the cellular populations that are infected by HIV or that harbor latent HIV proviruses is imperative in the attempts to address cure strategies, taking into account that HIV infection and latency in the CNS may differ considerably from those in the periphery. HIV replication in the CNS is reported to persist despite prolonged combination antiretroviral therapy due to the inability of the current antiretroviral drugs to penetrate and cross the blood–brain barrier. Consequently, as a result of sustained HIV replication in the CNS even in the face of combination antiretroviral therapy, there is a high incidence of HIV-associated neurocognitive disorders (HAND). This article, therefore, provides a comprehensive review of HIV transcriptional regulation, latency, and therapy in the CNS