3 research outputs found
Skin Prick Tests and Allergy Diagnosis
Skin testing remains an essential diagnostic tool in modern allergy practice.
A signifi cant variability has been reported regarding technical procedures, interpretation of results and documentation. This review has the aim of consolidating methodological recommendations through a critical analysis on past and recent data. This will allow a better understanding on skin prick test (SPT) history; technique; (contra-) indications; interpretation
of results; diagnostic pitfalls; adverse reactions; and variability factors
Synthesis and application of a bromomethyl substituted scaffold to be used for efficient optimization of anti-virulence activity
Pilicides are a class of compounds that attenuate virulence in Gram negative bacteria by blocking the
chaperone/usher pathway in Escherichia coli. It has also been shown that compounds derived from the
peptidomimetic scaffold that the pilicides are based on can prevent both Ab aggregation and curli
formation. To facilitate optimizations towards the different targets, a new synthetic platform has been
developed that enables fast and simple introduction of various substituents in position C-7 on the
peptidomimetic scaffold. Importantly, this strategy also enables introduction of previously unattainable
heteroatoms in this position. Pivotal to the synthetic strategy is the synthesis of a C-7 bromomethyl
substituted derivative of the ring-fused dihydrothiazolo 2-pyridone pilicide scaffold. From this versatile
and reactive intermediate various heteroatom-linked substituents could be introduced on the scaffold
including amines, ethers, amides and sulfonamides. In addition, carbon-carbon bonds could be introduced
to the sp3-hybridized bromomethyl substituted scaffold by SuzukieMiyaura cross couplings.
Evaluation of the 24 C-7 substituted compounds in whole-bacterial assays provided important structure
eactivity data and resulted in the identification of a number of new pilicides with activity as good or
better than those developed previousl