The \u3cem\u3elet-7\u3c/em\u3e MicroRNA Family Members \u3cem\u3emir\u3c/em\u3e-48, \u3cem\u3emir\u3c/em\u3e-84, and mir-241 Function Together to Regulate Developmental Timing in \u3cem\u3eCaenorhabditis elegans\u3c/em\u3e

The microRNA let-7 is a critical regulator of developmental timing events at the larval-to-adult transition in C. elegans. Recently, microRNAs with sequence similarity to let-7 have been identified. We find that doubly mutant animals lacking the let-7 family microRNA genes mir-48 and mir-84 exhibit retarded molting behavior and retarded adult gene expression in the hypodermis. Triply mutant animals lacking mir-48, mir-84, and mir-241 exhibit repetition of L2-stage events in addition to retarded adult-stage events. mir-48, mir-84, and mir-241 function together to control the L2-to-L3 transition, likely by base pairing to complementary sites in the hbl-1 3′ UTR and downregulating hbl-1 activity. Genetic analysis indicates that mir-48, mir-84, and mir-241 specify the timing of the L2-to-L3 transition in parallel to the heterochronic genes lin-28 and lin-46. These results indicate that let-7 family microRNAs function in combination to affect both early and late developmental timing decisions

A rapid, chromatography-free route to substituted acridine–isoalloxazine conjugates under microwave irradiation

Microwave irradiation was applied to a sequence of condensation reactions from readily available 9-chloroacridines to provide a range of novel acridine–isoalloxazine conjugates. The combination of these two moieties, both of biological interest, was achieved by a chromatography free route

Promissory note, 5 May 1851

https://egrove.olemiss.edu/aldrichcorr_c/1011/thumbnail.jp

Tax receipt, 1857

https://egrove.olemiss.edu/aldrichcorr_c/1136/thumbnail.jp

Implementation planning for UADPS-SP: a computer based supply and financial control system

Since its inception in 1963, the Uniform Automated Data Processing System for Stock Points (UADPS-SP) has grown steadily both in the scope of supply, accounting, and data processing functions it performs and in the numbers and types of activities it services. The basic purpose of this thesis is to provide guidance to prospective UADPS-SP customers on the preparations required for successful conversion of their local supply and accounting operations to one of the centrally-designed systems provided by the Fleet Material Support Office (FMSO) under the direction of the Naval Supply Systems Command (NAVSUP)http://archive.org/details/implementationpl00bohaLieutenant, Supply Corps, United States NavyLieutenant Commander, Supply Corps, United States NavyApproved for public release; distribution is unlimited

Microwave Components with MEMS Switches

RF MEMS switches with metal-metal contacts are being developed for microwave applications where broadband, high linearity performance is required. These switches provide less than 0.2 dB insertion loss through 40 GHz. This paper describes the integration of these switches into selected microwave components such as reconfigurable antenna elements, tunable filters, switched delay lines, and SPDT switches. Microwave and millimeter wave measured results from these circuits are presented

Association of acculturation with cardiac structure and function among Hispanics/Latinos: a cross-sectional analysis of the echocardiographic study of Latinos.

OBJECTIVE:Hispanics/Latinos, the largest immigrant population in the USA, undergo the process of acculturation and have a large burden of heart failure risk. Few studies have examined the association of acculturation on cardiac structure and function. DESIGN:Cross-sectional. SETTING:The Echocardiographic Study of Latinos. PARTICIPANTS:1818 Hispanic adult participants with baseline echocardiographic assessment and acculturation measured by the Short Acculturation Scale, nativity, age at immigration, length of US residence, generational status and language. PRIMARY AND SECONDARY OUTCOME MEASURES:Echocardiographic assessment of left atrial volume index (LAVI), left ventricular mass index (LVMI), early diastolic transmitral inflow and mitral annular velocities. RESULTS:The study population was predominantly Spanish-speaking and foreign-born with mean residence in the US of 22.7 years, mean age of 56.4 years; 50% had hypertension, 28% had diabetes and 44% had a body mass index &gt;30 kg/m2. Multivariable analyses demonstrated higher LAVI with increasing years of US residence. Foreign-born and first-generation participants had higher E/e' but lower LAVI and e' velocities compared with the second generation. Higher acculturation and income &gt;20K were associated with higher LVMI, LAVI and E/e' but lower e' velocities. Preferential Spanish-speakers with an income <20K had a higher E/e'. CONCLUSIONS:Acculturation was associated with abnormal cardiac structure and function, with some effect modification by socioeconomic status

Paper: Expert Viewers\u27 Preferences for Higher Frame Rate 3D Film

Recently the movie industry has been advocating the use of frame rates significantly higher than the traditional 24 frames per second. This higher frame rate theoretically improves the quality of motion portrayed in movies, and helps avoid motion blur, judder and other undesirable artifacts. Previously we reported that young adult audiences showed a clear preference for higher frame rates, particularly when contrasting 24 fps with 48 or 60 fps. We found little impact of shutter angle (frame exposure time) on viewers’ choices. In the current study we replicated this experiment with an audience composed of imaging professionals who work in the film and display industry who assess image quality as an aspect of their everyday occupation. These viewers were also on average older and thus could be expected to have attachments to the “film look” both through experience and training. We used stereoscopic 3D content, filmed and projected at multiple frame rates (24, 48 and 60 fps), with shutter angles ranging from 90◦ to 358◦, to evaluate viewer preferences. In paired-comparison experiments we assessed preferences along a set of five attributes (realism, motion smoothness, blur/clarity, quality of depth and overall preference). As with the young adults in the earlier study, the expert viewers showed a clear preference for higher frame rates, particularly when contrasting 24 fps with 48 or 60 fps. We found little impact of shutter angle on viewers’ choices, with the exception of one clip at 48 fps where there was a preference for larger shutter angle. However, this preference was found for the most dynamic “warrior” clip in the experts but in the slower moving “picnic” clip for the naïve viewers. These data confirm the advantages afforded by high-frame rate capture and presentation in a cinema context in both naïve audiences and experienced film professionals.https://source.sheridancollege.ca/centres_sirt_works/1002/thumbnail.jp

Most \u3cem\u3eCaenorhabditis elegans\u3c/em\u3e MicroRNAs are Individually Not Essential for Development or Viability

MicroRNAs (miRNAs), a large class of short noncoding RNAs found in many plants and animals, often act to post-transcriptionally inhibit gene expression. We report the generation of deletion mutations in 87 miRNA genes in Caenorhabditis elegans, expanding the number of mutated miRNA genes to 95, or 83% of known C. elegans miRNAs. We find that the majority of miRNAs are not essential for the viability or development of C. elegans, and mutations in most miRNA genes do not result in grossly abnormal phenotypes. These observations are consistent with the hypothesis that there is significant functional redundancy among miRNAs or among gene pathways regulated by miRNAs. This study represents the first comprehensive genetic analysis of miRNA function in any organism and provides a unique, permanent resource for the systematic study of miRNAs

Evidence that Viewers Prefer Higher Frame Rate Film

High frame rate (HFR) movie-making refers to the capture and projection of movies at frame rates several times higher than the traditional 24 frames per second. This higher frame rate theoretically improves the quality of motion portrayed in movies, and helps avoid motion blur, judder and other undesirable artefacts. However, there is considerable debate in the cinema industry regarding the acceptance of HFR content given anecdotal reports of hyper-realistic imagery that reveals too much set and costume detail. Despite the potential theoretical advantages, there has been little empirical investigation of the impact of high-frame rate techniques on the viewer experience. In this study we use stereoscopic 3D content, filmed and projected at multiple frame rates (24, 48 and 60 fps), with shutter angles ranging from 90 degrees to 358 degrees, to evaluate viewer preferences. In a paired-comparison paradigm we assessed preferences along a set of five attributes (realism, motion smoothness, blur/clarity, quality of depth and overall preference). The resulting data show a clear preference for higher frame rates, particularly when contrasting 24 fps with 48 or 60 fps. We found little impact of shutter angle on viewers’ choices, with the exception of one measure (motion smoothness) for one clip type. These data are the first empirical evidence of the advantages afforded by high frame rate capture and presentation in a cinema context.https://source.sheridancollege.ca/centres_sirt_works/1000/thumbnail.jp