Enhanced Retention In The Passive-Avoidance Task By 5-HT1A Receptor Blockade Is Not Associated With Increased Activity Of The Central Nucleus Of The Amygdala

The effect of blockade of S-HT1A receptors was investigated on (1) retention in a mildly aversive passive-avoidance task, and (2) spontaneous single-unit activity of central nucleus of the amygdala (CeA) neurons, a brain site implicated in modulation of retention. Systemic administration of the selective S-HT1A antagonist NAN-190 immediately after training markedly-and dose-dependently-facilitated retention in the passive-avoidance task; enhanced retention was time-dependent and was not attributable to variations in wattages of shock received by animals. Systemic administration of NAN-190 had mixed effects on spontaneous single-unit activity of CeA neurons recorded extracellularly in vivo; microiontophoretic application of S-HT, in contrast, consistently and potently suppressed CeA activity. The present findings-that S-HT1A receptor blockade by NAN-190 (1) enhances retention in the passive-avoidance task, and (2) does not consistently increase spontaneous neuronal activity of the CeA-provide evidence that a serotonergic system tonically inhibits modulation of retention in the passive-avoidance task through activation of the S-HT1A receptor subtype at brain sites located outside the CeA

Quantum Electrical Dipole in Triangular Systems: a Model for Spontaneous Polarity in Metal Clusters

Triangular symmetric molecules with mirror symmetry perpendicular to the 3-fold axis are forbidden to have a fixed electrical dipole moment. However, if the ground state is orbitally degenerate and lacks inversion symmetry, then a ``quantum'' dipole moment does exist. The system of 3 electrons in D_3h symmetry is our example. This system is realized in triatomic molecules like Na_3. Unlike the fixed dipole of a molecule like water, the quantum moment does not point in a fixed direction, but lies in the plane of the molecule and takes quantized values +/- mu_0 along any direction of measurement in the plane. An electric field F in the plane leads to a linear Stark splitting +/- mu_0 F}. We introduce a toy model to study the effect of Jahn-Teller distortions on the quantum dipole moment. We find that the quantum dipole property survives when the dynamic Jahn-Teller effect is included, if the distortion of the molecule is small. Linear Stark splittings are suppressed in low fields by molecular rotation, just as the linear Stark shift of water is suppressed, but will be revealed in moderately large applied fields and low temperatures. Coulomb correlations also give a partial suppression.Comment: 10 pages with 7 figures included; thoroughly revised with a new coauthor; final minor change

The Formation of Broad Line Clouds in the Accretion Shocks of Active Galactic Nuclei

Recent work on the gas dynamics in the Galactic Center has improved our understanding of the accretion processes in galactic nuclei, particularly with regard to properties such as the specific angular momentum distribution, density, and temperature of the inflowing plasma. This information can be valuable in trying to determine the origin of the Broad Line Region (BLR) in Active Galactic Nuclei (AGNs). In this paper, we explore various scenarios for the cloud formation based on the underlying principle that the source of plasma is ultimately that portion of the gas trapped by the central black hole from the interstellar medium. Based on what we know about the Galactic Center, it is likely that in highly dynamic environments such as this, the supply of matter is due mostly to stellar winds from the central cluster. Winds accreting onto a central black hole are subjected to several disturbances capable of producing shocks, including a Bondi-Hoyle flow, stellar wind-wind collisions, and turbulence. Shocked gas is initially compressed and heated out of thermal equilibrium with the ambient radiation field; a cooling instability sets in as the gas is cooled via inverse-Compton and bremsstrahlung processes. If the cooling time is less than the dynamical flow time through the shock region, the gas may clump to form the clouds responsible for broad line emission seen in many AGN spectra. Clouds produced by this process display the correct range of densities and velocity fields seen in broad emission lines. Very importantly, the cloud distribution agrees with the results of reverberation studies, in which it is seen that the central line peak responds slower to continuum changes than the line wings.Comment: 22 pages, 5 figure

A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture

Background. Bone engineering requires thicker three-dimensional constructs than the maximum thickness supported by standard cell-culture techniques (2 mm). A flow-perfusion bioreactor was developed to provide chemotransportation to thick (6 mm) scaffolds. Methods. Polyurethane scaffolds, seeded with murine preosteoblasts, were loaded into a novel bioreactor. Control scaffolds remained in static culture. Samples were harvested at days 2, 4, 6, and 8 and analyzed for cellular distribution, viability, metabolic activity, and density at the periphery and core. Results. By day 8, static scaffolds had a periphery cell density of 67% ± 5.0%, while in the core it was 0.3% ± 0.3%. Flow-perfused scaffolds demonstrated peripheral cell density of 94% ± 8.3% and core density of 76% ± 3.1% at day 8. Conclusions. Flow perfusion provides chemotransportation to thick scaffolds. This system may permit high throughput study of 3D tissues in vitro and enable prefabrication of biological constructs large enough to solve clinical problems

Field and intensity correlations in amplifying random media

We study local and nonlocal correlations of light transmitted through active random media. The conventional approach results in divergence of ensemble averaged correlation functions due to existence of lasing realizations. We introduce conditional average for correlation functions by omitting the divergent realizations. Our numerical simulation reveals that amplification does not affect local spatial correlation. The nonlocal intensity correlations are strongly magnified due to selective enhancement of the contributions from long propagation paths. We also show that by increasing gain, the average mode linewidth can be made smaller than the average mode spacing. This implies that light transport through a diffusive random system with gain could exhibit some similarities to that through a localized passive system, owing to dominant influence of the resonant modes with narrow width.Comment: 5 pages, 4 figure

Antenatal Steroid Therapy for Fetal Lung Maturation and the Subsequent Risk of Childhood Asthma: A Longitudinal Analysis

This study was designed to test the hypothesis that fetal exposure to corticosteroids in the antenatal period is an independent risk factor for the development of asthma in early childhood with little or no effect in later childhood. A population-based cohort study of all pregnant women who resided in Nova Scotia, Canada, and gave birth to a singleton fetus between 1989 and 1998 was undertaken. After a priori specified exclusions, 80,448 infants were available for analysis. Using linked health care utilization records, incident asthma cases developed after 36 months of age were identified. Extended Cox proportional hazards models were used to estimate hazard ratios while controlling for confounders. Exposure to corticosteroids during pregnancy was associated with a risk of asthma in childhood between 3–5 years of age: adjusted hazard ratio of 1.19 (95% confidence interval: 1.03, 1.39), with no association noted after 5 years of age: adjusted hazard ratio for 5–7 years was 1.06 (95% confidence interval: 0.86, 1.30) and for 8 or greater years was 0.74 (95% confidence interval: 0.54, 1.03). Antenatal steroid therapy appears to be an independent risk factor for the development of asthma between 3 and 5 years of age

Constraints on Cosmic Strings due to Black Holes Formed from Collapsed Cosmic String Loops

The cosmological features of primordial black holes formed from collapsed cosmic string loops are studied. Observational restrictions on a population of primordial black holes are used to restrict $f$, the fraction of cosmic string loops which collapse to form black holes, and $\mu$, the cosmic string mass-per-unit-length. Using a realistic model of cosmic strings, we find the strongest restriction on the parameters $f$ and $\mu$ is due to the energy density in $100 MeV$ photons radiated by the black holes. We also find that inert black hole remnants cannot serve as the dark matter. If earlier, crude estimates of $f$ are reliable, our results severely restrict $\mu$, and therefore limit the viability of the cosmic string large-scale structure scenario.Comment: (Plain Tex, uses tables.tex -- wrapped lines corrected), 11 pages, FERMILAB-Pub-93/137-

Phylogenetic Incongruence in E. coli O104: Understanding the Evolutionary Relationships of Emerging Pathogens in the Face of Homologous Recombination

Escherichia coli O104:H4 was identified as an emerging pathogen during the spring and summer of 2011 and was responsible for a widespread outbreak that resulted in the deaths of 50 people and sickened over 4075. Traditional phenotypic and genotypic assays, such as serotyping, pulsed field gel electrophoresis (PFGE), and multilocus sequence typing (MLST), permit identification and classification of bacterial pathogens, but cannot accurately resolve relationships among genotypically similar but pathotypically different isolates. To understand the evolutionary origins of E. coli O104:H4, we sequenced two strains isolated in Ontario, Canada. One was epidemiologically linked to the 2011 outbreak, and the second, unrelated isolate, was obtained in 2010. MLST analysis indicated that both isolates are of the same sequence type (ST678), but whole-genome sequencing revealed differences in chromosomal and plasmid content. Through comprehensive phylogenetic analysis of five O104:H4 ST678 genomes, we identified 167 genes in three gene clusters that have undergone homologous recombination with distantly related E. coli strains. These recombination events have resulted in unexpectedly high sequence diversity within the same sequence type. Failure to recognize or adjust for homologous recombination can result in phylogenetic incongruence. Understanding the extent of homologous recombination among different strains of the same sequence type may explain the pathotypic differences between the ON2010 and ON2011 strains and help shed new light on the emergence of this new pathogen

Gravitational wave bursts from cusps and kinks on cosmic strings

The strong beams of high-frequency gravitational waves (GW) emitted by cusps and kinks of cosmic strings are studied in detail. As a consequence of these beams, the stochastic ensemble of GW's generated by a cosmological network of oscillating loops is strongly non Gaussian, and includes occasional sharp bursts that stand above the ``confusion'' GW noise made of many smaller overlapping bursts. Even if only 10% of all string loops have cusps these bursts might be detectable by the planned GW detectors LIGO/VIRGO and LISA for string tensions as small as $G \mu \sim 10^{-13}$. In the implausible case where the average cusp number per loop oscillation is extremely small, the smaller bursts emitted by the ubiquitous kinks will be detectable by LISA for string tensions as small as $G \mu \sim 10^{-12}$. We show that the strongly non Gaussian nature of the stochastic GW's generated by strings modifies the usual derivation of constraints on $G \mu$ from pulsar timing experiments. In particular the usually considered ``rms GW background'' is, when G \mu \gaq 10^{-7}, an overestimate of the more relevant confusion GW noise because it includes rare, intense bursts. The consideration of the confusion GW noise suggests that a Grand Unified Theory (GUT) value $G \mu \sim 10^{-6}$ is compatible with existing pulsar data, and that a modest improvement in pulsar timing accuracy could detect the confusion noise coming from a network of cuspy string loops down to $G \mu \sim 10^{-11}$. The GW bursts discussed here might be accompanied by Gamma Ray Bursts.Comment: 24 pages, 3 figures, Revtex, submitted to Phys. Rev.

Policy Insights From the EMF 32 Study on U.S. Carbon Tax Scenarios

The Stanford Energy Modeling Forum exercise 32 (EMF 32) used 11 different models to assess emissions, energy, and economic outcomes from a plausible range of economy-wide carbon price policies to reduce carbon dioxide (CO2) emissions in the United States. Here we discuss the most policy-relevant results of the study, mindful of the strengths and weaknesses of current models. Across all models, carbon prices lead to significant reduc- tions in CO2 emissions and conventional pollutants, with the vast majority of the reductions occurring in the electricity sector. Importantly, emissions reductions do not significantly depend on the rebate or tax cut used to return revenues to the economy. Expected economic costs, as modeled by either GDP or welfare, are modest, but vary across models. These costs are offset by benefits from avoided climate damages and health benefits from reductions in conventional air pollution. Using revenues to reduce preexisting capital or labor taxes reduces costs in most models relative to lump-sum rebates, but the size of the cost reductions varies significantly. Devoting at least some revenue to household rebates can significantly reduce adverse impacts on low income households. Carbon prices at $25/ton or even lower levels cause significant shifts away from coal as an energy source with responses of other energy sources highly dependent upon technology cost assumptions. Beyond 2030, we conclude that model uncertainties are too large to make quantitative results useful for near-term policy design. We close by describing recommendations for policymakers on interacting with model results in the future