‘Everyone thought I was a very very bad person… no one want to know you like the nurses and doctors’:using focus groups to elicit the views of adults with learning disability who use challenging behaviour services

and Tables S1–S3. (PDF 3090 kb

An epigenetic clock for gestational age at birth based on blood methylation data

Background: Gestational age is often used as a proxy for developmental maturity by clinicians and researchers alike. DNA methylation has previously been shown to be associated with age and has been used to accurately estimate chronological age in children and adults. In the current study, we examine whether DNA methylation in cord blood can be used to estimate gestational age at birth. Results: We find that gestational age can be accurately estimated from DNA methylation of neonatal cord blood and blood spot samples. We calculate a DNA methylation gestational age using 148 CpG sites selected through elastic net regression in six training datasets. We evaluate predictive accuracy in nine testing datasets and find that the accuracy of the DNA methylation gestational age is consistent with that of gestational age estimates based on established methods, such as ultrasound. We also find that an increased DNA methylation gestational age relative to clinical gestational age is associated with birthweight independent of gestational age, sex, and ancestry. Conclusions: DNA methylation can be used to accurately estimate gestational age at or near birth and may provide additional information relevant to developmental stage. Further studies of this predictor are warranted to determine its utility in clinical settings and for research purposes. When clinical estimates are available this measure may increase accuracy in the testing of hypotheses related to developmental age and other early life circumstances.Peer reviewe

Additional file 3: Table S3. of Maternal urinary phthalates and sex-specific placental mRNA levels in an urban birth cohort

Z-score differences in placental mRNAs between pregnancies with and without complications. (DOCX 76 kb

Additional file 2: Table S2. of Maternal urinary phthalates and sex-specific placental mRNA levels in an urban birth cohort

Associations of placental gene expression in female and male placentas with maternal urinary monoester phthalates. (DOCX 29 kb

Cumulative lifetime maternal stress and epigenome-wide placental DNA methylation in the PRISM cohort

<p>Evolving evidence links maternal stress exposure to changes in placental DNA methylation of specific genes regulating placental function that may have implications for the programming of a host of chronic disorders. Few studies have implemented an epigenome-wide approach. Using the Infinium HumanMethylation450 BeadChip (450K), we investigated epigenome-wide placental DNA methylation in relation to maternal experiences of traumatic and non-traumatic stressors over her lifetime assessed using the Life Stressor Checklist-Revised (LSC-R) survey (n = 207). We found differential DNA methylation at epigenome-wide statistical significance (FDR = 0.05) for 112 CpGs. Additionally, we observed three clusters that exhibited differential methylation in response to high maternal lifetime stress. Enrichment analyses, conducted at an FDR = 0.20, revealed lysine degradation to be the most significant pathway associated with maternal lifetimes stress exposure. Targeted enrichment analyses of the three largest clusters of probes, identified using the gap statistic, were enriched for genes associated with endocytosis (i.e., <i>SMAP1, ANKFY1</i>), tight junctions (i.e., <i>EPB41L4B</i>), and metabolic pathways (i.e., <i>INPP5E, EEF1B2</i>). These pathways, also identified in the top 10 KEGG pathways associated with maternal lifetime stress exposure, play important roles in multiple physiological functions necessary for proper fetal development. Further, two genes were identified to exhibit multiple probes associated with maternal lifetime stress (i.e., <i>ANKFY1, TM6SF1</i>). The methylation status of the probes belonging to each cluster and/or genes exhibiting multiple hits, may play a role in the pathogenesis of adverse health outcomes in children born to mothers with increased lifetime stress exposure.</p

Additional file 1: of Birth weight-for-gestational age is associated with DNA methylation at birth and in childhood

Descriptive characteristics of methylation levels in birth weight-for-gestational age (BW/GA)-associated DNA methylation sites in venous umbilical cord blood at delivery, among 476 participants in Project Viva. (XLSX 12 kb

Intervention dans le Podcast les Arts du FLE pour faire le point sur la perspective actionnelle en classe de langues

Ce podcast est disponible en ligne, à l'adresse suivante :https://agi.to/podcast/arts-du-fle-08-actionne-ton-approche/Ce numéro 8 des Arts du FLE (émission spécialisée en FLE) propose un dossier spécial approches pédagogiques et perspective actionnelle. J'en suis l'invitée et j'y réponds de manière détaillée pendant près d'une heure aux questions de l'animateur et créateur de ce Podcast, Sébastien Durietz (en poste actuellement à l'ONU, à New-York)

Additional file 6: Figures S1–S4. of DNA methylation signatures of chronic low-grade inflammation are associated with complex diseases

(PDF 1036 kb

Additional file 10: Table S9. of DNA methylation signatures of chronic low-grade inflammation are associated with complex diseases

Significant associations between DNA methylation and corresponding nearby genetic variants and between the genetic variant and CRP in the largest published GWAS of CRP (n = 66,185). (XLSX 12 kb

Additional file 9: Table S8. of DNA methylation signatures of chronic low-grade inflammation are associated with complex diseases

Significant methylation-expression results and corresponding expression-CRP results for the replicated CpGs. (XLSX 11 kb