Role of Prebiotic, Probiotic and Symbiotic Diets on Bacterial proliferation in Feed and Intestine of African (Clarias gariepinus) Catfish

The influence of prebiotic, probiotic and symbiotic diet on microbial proliferation was studied using an in vitro method. In the present trial, formulated diets were supplemented with prebiotic (Sargassum muticum), probiotic (Parkia biglobosa) and combination of Parkia biglobosa and Sargassum muticum (symbiotic diet). Bacteria proliferation in supplemented feeds, small and large intestine of the African catfish, Clarias gariepinus fed the formulated diets were evaluated. The feeding trial that lasted 12 weeks was conducted in plastic aquaria, with each treatment replicated three times. A control diet containing only the feed ingredients was also formulated and fed for the same period. The result showed bacteria proliferation was lowest in the control diet and highest in feed supplemented with prebiotic. Low bacteria proliferation was observed in the small intestine of fish fed symbiotic diet while highest proliferation was recorded in the fish fed prebiotic diet. The result also revealed the lowest bacteria proliferation in the large intestine of fish fed symbiotic diet and highest in fish fed probiotic diet. Using the cell morphology and biochemical characteristics of bacteria isolates in supplemented feed, Clarias gariepinus small and large intestine, the result indicated the presence of some Lactic Acid Bacteria (LAB) known to produce a variety of antimicrobial substances which are able to stop the development of foodborne diseases by inhibiting the growth of food spoilage and pathogenic organisms

Performance evaluation of 200W solar photovoltaic panel considering Bauchi microclimatic conditions

Measurement and modeling of broadband and spectral terrestrial solar radiation is important for the evaluation and deployment of solar renewable energy systems. This paper focuses on optimizing the performance of 200W solar module taking into consideration the local climatic conditions of Bauchi locality. The uncertainty in life cycle savings for solar thermal and photovoltaic (PV) systems as linearly correlated with uncertainty in solar resource data. These uncertainties paved way for the need to conduct a critical assessment of the resource. Assessment of the solar resource for these technologies rely upon measured data, where available. In this paper, we present the development of mathematical model of photovoltaic solar cells based on their detailed single diode equivalent circuit representation. Pertinent simulation models for PV solar module both for an ideal weather situation and for taking into consideration the effects of microclimatic conditions that prevail in Bauchi as evaluated and compared with benchmarks available. The complete model of the PV system was implemented using MATLAB/Simulink platform. The standard characteristic curves for the 200W solar panel are as presented. The simulation of the ideal PV system made use of standard test conditions (STC) to facilitate comparison with the existing benchmark results in the literature. The analysis of the characteristics performance curves returned an average VOC = 42.9v and ISC = 4.21A. The simulation results further revealed that the power delivered by the 200W monocrystalline solar module of 144.3W @620W/m2, 35ºC as recorded for Bauchi under all climatic conditions as evaluated. The benchmark values obtained in the laboratory are VOC = 45.5V, ISC = 5.92A and 200-W under the Standard test condition (STC) conditions of cell temperature 25ºC, solar irradiance of 1000W/m2 and air mass (AM) of 1.5. The average conversion efficiency and fill factor as evaluated are 0.77 and 16% respectively. This result agrees with the benchmark of module efficiency of >15.66% recorded at STC. The results conclusively reveal that the microclimate of a locality essentially affects the performances of solar PV systems deployed to each location on the globe. Therefore, utilization of these parameters is essential for consideration in the design of solar systems in all localities

Partial Ordering in Soft Set Context

In [1], [2], [3], [4], [5], [6] and [7] basic introduction of soft set is discussed with examples. The main aim of this paper is develop partial ordering in soft set context.

Prevalence of extended spectrum β-lactamases (esbls) among enterobacteriaceae in murtala mohammed specialist hospital, Kano, Nigeria

Confirmed variants of enterobacteriaceae isolated from 143 patients that attended Murtala Mohammed Specialist Hospital Kano, were screened for extended spectrum β-lactamases (ESBLs) production using Clinical Laboratory Standards Institute (CLSI) breakpoint. Suspected ESBLs producers were subjected to confirmation using Disc Replacement Method (DRM). Standard discs of augmentin {AMC 30μg (Oxoid, England)}, ceftriaxone {AUF 30μg (Oxoid, England)} and ceftazidime {RP 30μg (Oxoid, England)} were used in the screening. Of the 143 isolates screened, 114 (79.72%) were Gram negative isolates belonging to the family enterobacteriaceae. Among the enterobacteriaceae isolates screened, the results of CLSI breakpoint test showed that 76 (66.7%) were ESBLs producers viz: Citrobacter spp. (3), Enterobacter spp. (2), E. coli (28), Klebsiella spp. (18), Morganella morganii (7), Proteus spp. (13), Salmonella spp. (1), Serratia spp. (1), Shigella spp. (2) and Yersinia spp (1). On subjecting the CLSI positive isolates to DRM, only 47 (41.2%) were confirmed ESBLs producers. These include; Citrobacter spp. (1), E. coli (20), Klebsiella spp. (12), Morganella morganii (4), Proteus spp. (8), Salmonella spp. (1) and Shigella spp. (1). ESBLs occur at an alarming rate among enterobacteriaceae isolates in Kano which calls for government intervention in the healthcare setting

Reactive Power and Voltage Control of the Nigerian Grid System using Micro-Genetic Algorithm

In this paper, a micro-genetic based approach to the optimization of reactive power and voltage profiles improvement and real power loss minimization is presented. The reactive power control devices such as generators, tap positions of on-load tap changer of transformers, shunt reactors are used to correct voltage limits violations while simultaneously reducing the system real power losses. Genetic algorithms (GAs) are well-known global search techniques anchored on the mechanisms of natural selection and genetics. Because of the time intensive nature of the conventional GA, the micro-GA is proposed as a more time efficient alternative. The feasibility and effectiveness of the developed algorithm is tested and verified on the Nigerian grid power system for three case studies scenarios preset in the power world simulator. The far-reaching simulation results that validate the effectiveness of the developed tool are presented and discussed in depth

Synthesis, characterization, in-vitro anti-inflammatory and antimicrobial screening of metal(II) mixed diclofenac and acetaminophen complexes

Mixed ligand complexes derived from diclofenac potassium salt (Kdc) and acetaminophen (ace) has been synthesized and proposed to have a general formula [MB] where M = Co2+, Ni2+, Cu2+ and Zn2+and B = (ace)(dc)(H2O)2 except for Mn2+ complex which exists as [Mn(ace)(dc)OH2]. The complexes were characterized by solubility, melting point, conductivity, elemental analyses, UV-Vis, FT-IR spectroscopy, X-ray powder diffraction (XRPD) study and magnetic susceptibility measurement. Electronic absorption spectra data are characteristic of octahedral structures for [MB]. The IR spectra revealed a bidentate coordination mode. In acetaminophen, the nitrogen and carbonyl-O atoms of the amide group were involved while the carboxylate oxygen atoms of potassium diclofenac were used; typical of a carboxylic acid derivative. The compounds were screened for in-vitro anti-inflammatory activity by inhibition of albumin denaturation assay and antimicrobial activity against bacteria strains: Bacillus subtilis, Bacillus anthrax, Escherichia coli, Salmonella typhi and a fungus Aspergillus niger. Some of the tested compounds showed moderate anti-inflammatory activity when compared to the standard drug diclofenac potassium salt. The in-vitro antimicrobial screening revealed an increased activity of the complexes against the bacteria isolates compared to the free ligands.                     KEY WORDS: Metal(II) ion, NSAIDs, Anti-inflammatory activity, Diclofenac potassium salt, XRPD, Antimicrobial activity   Bull. Chem. Soc. Ethiop. 2021, 35(1), 77-86. DOI: https://dx.doi.org/10.4314/bcse.v35i1.

In silico proteomic analysis provides insights into phylogenomics and plant biomass deconstruction potentials of the Tremelalles

Basidiomycetes populate a wide range of ecological niches but unlike ascomycetes, their capabilities to decay plant polymers and their potential for biotechnological approaches receive less attention. Particularly, identification and isolation of CAZymes is of biotechnological relevance and has the potential to improve the cache of currently available commercial enzyme cocktails toward enhanced plant biomass utilization. The order Tremellales comprises phylogenetically diverse fungi living as human pathogens, mycoparasites, saprophytes or associated with insects. Here, we have employed comparative genomics approaches to highlight the phylogenomic relationships among thirty-five Tremellales and to identify putative enzymes of biotechnological interest encoded on their genomes. Evaluation of the predicted proteomes of the thirty-five Tremellales revealed 6,918 putative carbohydrate-active enzymes (CAZYmes) and 7,066 peptidases. Two soil isolates, Saitozyma podzolica DSM 27192 and Cryptococcus sp. JCM 24511, show higher numbers harboring an average of 317 compared to a range of 267–121 CAZYmes for the rest of the strains. Similarly, the proteomes of the two soil isolates along with two plant associated strains contain higher number of peptidases sharing an average of 234 peptidases compared to a range of 226–167 for the rest of the strains. Despite these huge differences and the apparent enrichment of these enzymes among the soil isolates, the data revealed a diversity of the various enzyme families that does not reflect specific habitat type. Growth experiment on various carbohydrates to validate the predictions provides support for this view. Overall, the data indicates that the Tremellales could serve as a rich source of both CAZYmes and peptidases with wide range of potential biotechnological relevance

Performance of metakaolin based geopolymer concrete at elevated temperature

Due to the carbon dioxide emission arising from the production of cement, alternative concrete that is environmentally friendly such as metakaolin geopolymer concrete have been developed. However, the performance of metakaolin based geopolymer concrete (MKGC) when exposed to aggressive environment particularly elevated temperature has not been investigated. Therefore, this paper assessed the performance of MKGC exposed to elevated temperatures. MKGC cube specimens of grade 25 were produced using a mix ratio of 1:1.58:3.71.After preparing the specimens, they were placed in an electric oven at a temperature of 60oC for 24 hours. Thereafter, the specimens were stored in the laboratory at ambient temperature for 28 days. The specimens were then exposed to elevated temperatures of 200, 400, 600 and 800oC. After exposure to elevated temperatures, the MKGC specimens were subjected to compressive strength, water absorption and abrasion resistance tests. Results show that at 600 and 800oC, the MKGC lost a compressive strength of 59.69% and 71.71% respectively. Higher water absorption and lower abrasion resistance were also observed. Keywords: Cement, Compressive Strength, Metakaolin Concrete, Elevated Temperature