Investigating the antioxidant properties and rutin content of Sea buckthorn (Hippophae rhamnoides L.) leaves and branches

The present study focused on the antioxidant properties and rutin content of leaves and branches of Hippophae rhamnoides L. (Sea buckthorn) in Turkey. Dried leaves (leaf tea), processed (PB) and unprocessed branches (UB) of Sea buckthorn (SBT) were extracted with ethanol and prepared in forms of aqueous extract (AE). All samples were analyzed for their contents of rutin, total phenolics (TPC), total flavonoids (TFC) and total antioxidant capacity by using DPPH and CUPRAC methods. TPC of leaves from ethanolic extracts (EE) were significantly higher than UB and PB extracts. The DPPH scavenging activity of extracts ranged from 41.93 ± 3.57 and 132.43 ± 6.57 mg trolox equivalent (TE)/g, and the antioxidant capacity measured with CUPRAC method were in between 129.4 ± 18.1 and 538.5 ± 34.8 mg TE/g. Both EE and AE of leaf samples had significantly higher rutin content compared to the UB and PB samples. Taking the high antioxidant and rutin content of leaves into account and with respect to their positive health effects, consumption of SBT as a herbal tea should be investigated.Keywords: Sea buckthorn, Hippophae rhamnoides, leaves, antioxidant, phenolic profile, rutin conten

In vitro Cytotoxicity Evaluation of Dandelion Root Ethanol Extract on PANC-1 Cell Line

A challenging diagnosis is the outcome of pancreatic cancer's aggressivity and malignant character. This type of cancer is mostly detected in the metastatic stage. Current treatment options have various side effects, result in the survival rates and quality of life of patients are extremely decreased. Dandelion (Taraxacum officinale (L.) Weber ex F.H. Wigg.)  is one of the natural products that has the potential to be therapeutic against a wide range of diseases. This study aimed to evaluate the potential of ethanol extract of dandelion root (DRE) as an alternative anticancer agent. Based on this purpose, milk-rich dandelion roots were collected, cut into small pieces, and then extracted using Soxhlet in the presence of 70% ethanol. The cytotoxic effect of the DRE at certain doses (10, 5, and 2.5 mg/mL) was determined by the Microtetrazolium (MTT) assay for 24, 48, and 72 hours, and half-maximal inhibitory concentration (IC)50 values for these incubation periods were found to be 10 mg/mL, 6.80 mg/mL and 6 mg/mL (p0.001), respectively. As a result, DRE may reduce cell viability when applied to aggressive pancreatic cancer cells and may have the potential to be an alternative anticancer agent. This potential can be clarified with further studies