Construção de uma câmara para monitoramento in situ do processo de secagem de geis e sólidos porosos

The present work deals with the design and construction of an equipment for studying the drying process of gels and solids. The equipment was built using mainly a commercial oven and others peripherals for programmed control of temperature, humidity and data acquisition. The system allows the in situ monitoring of the gel drying, registering the weight loss, and the shrinkage of sample and the oven temperatures. This set of allowed parameters makes possible to establish the main steps involved in the drying of meso and nanostructured porous materials

Trenzado y alfabetizaciones: informes de un proyecto de Investigación y Práctica Pedagógica en la Escuela Tupinikim de Aldeia Comboios - Aracruz/ES

O presente relato nasce de um projeto pedagógico desenvolvido na Aldeia Tupinikim de Comboios, município de Aracruz- ES, durante a disciplina Projetos de Pesquisa e Prática Pedagógica – PPPP III, no curso de Licenciatura Intercultural Indígena Tupinikim e Guarani, do Programa de Licenciaturas Indígenas – PROLIND, oferecido pela Universidade Federal do Espírito Santo – UFES. O projeto teve como objetivos: Pesquisar com os mais velhos os tipos de trançados, colaborando na reafirmação cultural do nosso povo Tupiniquim; Reconhecer os tipos de trançados Tupiniquim; Confeccionar alguns tipos de trançados; Produzir textos coletivos a partir dos trançados e analisar o processo de letramento a partir de atividades contextualizadas. O projeto se apoiou metodologicamente na pesquisa-ação com intuito de identificar as técnicas e manuseios dos trançados Tupinikim, construindo momentos de partilhas com os anciãos e intercâmbios entre outras aldeias Tupinikim. Palavras-chave: Trançados; Letramentos; Povo Tupinkim; PROLIND.The present report is born from a pedagogical project developed in the Tupinikim Village of Comboios, municipality of Aracruz-ES, during the discipline Projects of Research and Pedagogical Practice - PPPP III, in the course of Indigenous Licentiate Tupinikim and Guarani, PROLIND, offered by the Federal University of Espírito Santo - UFES. The project had as objectives: To research with the older the types of braids, collaborating in the cultural reaffirmation of our Tupiniquim people; Recognize the types of Tupiniquim braids; Make some types of braids; Produce collective texts from the braids and analyze the process of literacy from contextualized activities. The project was methodologically based on action research in order to identify the techniques and manipulations of the Tupinikim braids, building moments of sharing with the elders and exchanges among other Tupinikim villages. Keywords: Braided; Lettering; Tupinkim people; PROLIND.Este informe es el resultado de un proyecto pedagógico desarrollado en la aldea Tupinikim de Comboios, municipio de Aracruz-ES, durante la disciplina Proyectos de investigación y práctica pedagógica - PPPP III, en el curso de licenciatura intercultural indígena Tupinikim y Guaraní, del Programa de licenciatura indígena - PROLIND, ofrecido por la Universidad Federal de Espírito Santo - UFES. El proyecto tenía como objetivos: investigar con los ancianos los tipos de trenzas, colaborando en la reafirmación cultural de nuestra gente tupiniquim; Reconocer los tipos de Tupiniquim trenzado; Hacer algunos tipos de trenzas; Produzca textos colectivos basados ​​en los hilos y analice el proceso de alfabetización basado en actividades contextualizadas. El proyecto se basó metodológicamente en la investigación de acción para identificar las técnicas y el manejo de las trenzas Tupinikim, creando momentos de intercambio con los ancianos e intercambios entre otras aldeas Tupinikim.Palabras clave: trenzado; Alfabetizaciones; Pueblo tupinkim; PROLIND

Euphol, a tetracyclic triterpene, from Euphorbia tirucalli induces autophagy and sensitizes temozolomide cytotoxicity on glioblastoma cells

Glioblastoma (GBM) is the most frequent and aggressive type of brain tumor. There are limited therapeutic options for GBM so that new and effective agents are urgently needed. Euphol is a tetracyclic triterpene alcohol, and it is the main constituent of the sap of the medicinal plant Euphorbia tirucalli. We previously identified anti-cancer activity in euphol based on the cytotoxicity screening of 73 human cancer cells. We now expand the toxicological screening of the inhibitory effect and bioactivity of euphol using two additional glioma primary cultures. Euphol exposure showed similar cytotoxicity against primary glioma cultures compared to commercial glioma cells. Euphol has concentration-dependent cytotoxic effects on cancer cell lines, with more than a five-fold difference in the IC50 values in some cell lines. Euphol treatment had a higher selective cytotoxicity index (0.64-3.36) than temozolomide (0.11-1.13) and reduced both proliferation and cell motility. However, no effect was found on cell cycle distribution, invasion and colony formation. Importantly, the expression of the autophagy-associated protein LC3-II and acidic vesicular organelle formation were markedly increased, with Bafilomycin A1 potentiating cytotoxicity. Finally, euphol also exhibited antitumoral and antiangiogenic activity in vivo, using the chicken chorioallantoic membrane assay, with synergistic temozolomide interactions in most cell lines. In conclusion, euphol exerted in vitro and in vivo cytotoxicity against glioma cells, through several cancer pathways, including the activation of autophagy-associated cell death. These findings provide experimental support for further development of euphol as a novel therapeutic agent for GBM, either alone or in combination chemotherapy.The work was supported by the Amazonia Fitomedicamentos (FITO05/2012) Ltda. and Barretos Cancer Hospital, all from Brazil

Serotype influences on dengue severity: a cross-sectional study on 485 confirmed dengue cases in Vitória, Brazil

Abstract Background Dengue is caused by a RNA virus of the family Flaviviridae, which presents four serotypes (DENV-1 to DENV-4) capable of inducing hemorrhage. The purpose of this study was to evaluate the influence of serotype on the outcome of dengue. Methods This cross-sectional study included data from dengue cases with serotyping results that occurred between 2009 and 2013 in Vitória, Espírito Santo, Brazil. Data were accessed through the Information System for Notifiable Diseases. Chi-square test, Fisher exact test, Mann–Whitney U test, and logistic regression were performed to assess associations between different serotypes and dengue severity, while considering gender and age. Results The sample consisted of 485 laboratory confirmed dengue cases, of which 46.4 % were females, with median age of 26 years. Regarding overall samples, 77.3 % were caused by DENV-1, 16.1 % by DENV-4, 6.4 % by DENV-2, and 0.2 % by DENV-3. Severe dengue affected 6.6 % of all cases, of which 32.3 % of the cases caused by DENV-2, 6.4 % of those caused by DENV-4, 4.5 % of those caused by DENV-1, and none of those caused by DENV-3. Severe dengue was found to be seven times more frequent among cases of DENV-2 than among those of the other serotypes. Conclusions The present study found that cases of DENV-2 had a higher proportion of severe dengue than among those of DENV-1 and DENV-4. Consequently, early detection of serotypes circulating in the territory could be an important approach to prevent increasing numbers of severe outcomes during dengue outbreaks by predicting the health support needed for early diagnoses and treatment of dengue cases