39,494 research outputs found
Band-edge Bilayer Plasmonic Nanostructure for Surface Enhanced Raman Spectroscopy
Spectroscopic analysis of large biomolecules is critical in a number of
applications, including medical diagnostics and label-free biosensing.
Recently, it has been shown that Raman spectroscopy of proteins can be used to
diagnose some diseases, including a few types of cancer. These experiments have
however been performed using traditional Raman spectroscopy and the development
of the Surface enhanced Raman spectroscopy (SERS) assays suitable for large
biomolecules could lead to a substantial decrease in the amount of specimen
necessary for these experiments. We present a new method to achieve high local
field enhancement in surface enhanced Raman spectroscopy through the
simultaneous adjustment of the lattice plasmons and localized surface plasmon
polaritons, in a periodic bilayer nanoantenna array resulting in a high
enhancement factor over the sensing area, with relatively high uniformity. The
proposed plasmonic nanostructure is comprised of two interacting nanoantenna
layers, providing a sharp band-edge lattice plasmon mode and a wide-band
localized surface plasmon for the separate enhancement of the pump and emitted
Raman signals. We demonstrate the application of the proposed nanostructure for
the spectral analysis of large biomolecules by binding a protein (streptavidin)
selectively on the hot-spots between the two stacked layers, using a low
concentration solution (100 nM) and we successfully acquire its SERS spectrum
Squeezed States and Hermite polynomials in a Complex Variable
Following the lines of the recent paper of J.-P. Gazeau and F. H. Szafraniec
[J. Phys. A: Math. Theor. 44, 495201 (2011)], we construct here three types of
coherent states, related to the Hermite polynomials in a complex variable which
are orthogonal with respect to a non-rotationally invariant measure. We
investigate relations between these coherent states and obtain the relationship
between them and the squeezed states of quantum optics. We also obtain a second
realization of the canonical coherent states in the Bargmann space of analytic
functions, in terms of a squeezed basis. All this is done in the flavor of the
classical approach of V. Bargmann [Commun. Pur. Appl. Math. 14, 187 (1961)].Comment: 15 page
Regular subspaces of a quaternionic Hilbert space from quaternionic Hermite polynomials and associated coherent states
We define quaternionic Hermite polynomials by analogy with two families of
complex Hermite polynomials. As in the complex case, these polynomials
consatitute orthogonal families of vectors in ambient quaternionic
-spaces. Using these polynomials, we then define regular and anti-regular
subspaces of these -spaces, the associated reproducing kernels and the
ensuing quaternionic coherent states
Morbidity Pattern Among Out-Patients Attending Urban Health Training Centre in Srinagar
The current study was designed to identify the morbidity pattern of out-patients attending Urban Health Training Centre in an urban area of a medical college in Srinagar, Pauri Garhwal district, Uttarakhand, North India. The present study record-based retrospective study was conducted among the out-patients attending the regular clinic at the Urban Health Training Centre, of a medical college in Srinagar city of Uttarakhand State of North India during the study period of one year in 2014. Data was retrieved from the OPD registers maintained at the clinic. Data was collected pertaining to socio-demographic profile, morbidity details and treatment pattern. Diseases were identified using the International Classification of Diseases (ICD-10) code. Descriptive analysis was done. During the study period, a total of 9343 subjects attended the OPD. Among them, majority of them (60%) were females. More than half (56 %) belonged to the age group of 35-65 year age-group. The association of disease classification was found to be statistically significant with respect to gender. The leading morbidity of communicable disease was found to be certain infectious and parasitic diseases especially Typhoid whereas musculoskeletal system and connective tissue disorders were the most common cause among morbidity due to NCDs. Out of all, typhoid was found to cause maximum of morbidity among the subjects. The present study highlights the morbidity pattern of communicable and NCDs among the population of hilly areas of Garhwal, Uttarakhand India. Priority should be preferred for the regular tracking of diseases in terms of preventive and promotive aspects. Morbidity in the out- door clinics reflects the emerging trend of mixed disease spectrum burden comprising communicable and non-communicable diseases
Pan-squamous genomic profiling stratified by anatomic tumor site and viral association
Background: Squamous cell carcinomas (SCC) have diverse anatomic etiologies but may share common genomic biomarkers. We profiled 7,871 unique SCCs across nine anatomic sites to investigate commonality in genomic alterations (GA), tumor mutational burden (TMB), human papillomavirus (HPV) association, and mutational signatures.
Methods: Tissue from over 8,100 unique SCC samples originating from nine anatomic sites (anogenital (anus, cervix, penis, vagina, vulva), esophagus, head and neck, lung, and skin) were sequenced by hybrid capture-based comprehensive genomic profiling to evaluate GA and TMB. About 3% of non-cutaneous SCC samples had UV signatures, indicative of potential primary site misdiagnoses, and were filtered from the analysis. Detection of HPV, including high-risk strains 16, 18, 31, 33, and 45, was implemented through de novo assembly of non-human sequencing reads and BLASTn comparison against all viral nucleotide sequences in the NCBI database.
Results: The proportion of HPV+ patients by anatomic site varied, with the highest being anal (91%) and cervical (83%). The mutational landscape of each cohort was similar, regardless of anatomic origin, but clustered based on HPV status. The largest differences in GA frequency as stratified by HPV- vs. HPV+ were TP53 (87% vs. 12%), CDKN2A (45% vs. 6%), and PIK3CA (22% vs. 33%). The median TMB in cases originating from HPV-associated sites was similar, regardless of HPV status. Higher median TMB was observed in lung and skin cases, which exhibited significant enrichment of mutational signatures indicative of tobacco- and UV-induced DNA damage, respectively.
Conclusions: HPV+ and HPV- SCC populations have distinct genomic profiles and, for the latter, anatomic site is correlated with TMB distribution, secondary to associated carcinogen exposure. As such, biomarkers such as TMB and UV signature can provide unexpected insight into site of origin misdiagnoses and may correlate with benefit from immune checkpoint inhibitors
Perspectives: Quantum Mechanics on Phase Space
The basic ideas in the theory of quantum mechanics on phase space are
illustrated through an introduction of generalities, which seem to underlie
most if not all such formulations and follow with examples taken primarily from
kinematical particle model descriptions exhibiting either Galileian or
Lorentzian symmetry. The structures of fundamental importance are the relevant
(Lie) groups of symmetries and their homogeneous (and associated) spaces that,
in the situations of interest, also possess Hamiltonian structures. Comments
are made on the relation between the theory outlined and a recent paper by
Carmeli, Cassinelli, Toigo, and Vacchini.Comment: "Quantum Structures 2004" - Meeting of the International Quantum
Structures Association; Denver, Colorado; 17-22 July, 200
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