Ecotoxicological Effects of Ibuprofen on Plant Growth of Vigna unguiculata L.

Despite the prevalence of the common pharmaceutical ibuprofen (IBU) in water and sediments worldwide, the effects of IBU on plants are largely unknown. This study was designed to assess the ecotoxicological effects of emerging pharmaceutical pollutant IBU on plant growth and development in a series of toxicity experiments using cowpea (Vigna unguiculata). Plant growth parameters (morphological and physicochemical) were investigated under a series of IBU concentrations (0, 400, 800, 1200, 1600, 2000 ppm IBU). IBU exposure reduced the shoot and root lengths, fresh and dry weights, leaf area, and chlorophyll a and b, carotenoid, total chlorophyll, mineral (K and Mg), glutathione reductase, and soluble protein contents. Simultaneously, increases in Ca and Mn contents, sodium translocation from roots to shoots, H2O2, malondialdehyde, superoxide dismutase, catalase, ascorbate peroxidase, and IBU uptake were observed. The amount of bioaccumulated IBU varied between 7% and 8%. IBU was translocated from roots to shoots with a translocation factor of 3–16%. The IC50 values for biomass and plant length were 1253 and 1955 ppm IBU, respectively, which is much higher than the reported levels of IBU in the environment. This study demonstrates that cowpea plants develop several morphological and physicochemical adaptations to cope under ibuprofen stress; environmentally relevant concentrations of IBU are unlikely to produce negative impacts.The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding Research group No. (RGP-199). The authors thank the Deanship of Scientific Research and RSSU at King Saud University for their technical support.Peer reviewe

Heteroleptic Copper(I) Complexes of "Scorpionate" Bis-pyrazolyl Carboxylate Ligand with Auxiliary Phosphine as Potential Anticancer Agents: An Insight into Cytotoxic Mode

New copper(I) complexes [CuCl(PPh3)(L)] (1: L = LA = 4-carboxyphenyl)bis(3,5-dimethylpyrazolyl)methane; (2: L = LB = 3-carboxyphenyl)bis(3,5-dimethylpyrazolyl)methane) were prepared and characterised by elemental analysis and various spectroscopic techniques such as FT-IR, NMR, UV-Vis, and ESI-MS. The molecular structures of complexes 1 and 2 were analyzed by theoretical B3LYP/DFT method. Furthermore, in vitro DNA binding studies were carried out to check the ability of complexes 1 and 2 to interact with native calf thymus DNA (CT-DNA) using absorption titration, fluorescence quenching and circular dichroism, which is indicative of more avid binding of the complex 1. Moreover, DNA mobility assay was also conducted to study the concentration-dependent cleavage pattern of pBR322 DNA by complex 1, and the role of ROS species to have a mechanistic insight on the cleavage pattern, which ascertained substantial roles by both hydrolytic and oxidative pathways. Additionally, we analyzed the potential of the interaction of complex 1 with DNA and enzyme (Topo I and II) with the aid of molecular modeling. Furthermore, cytotoxic activity of complex 1 was tested against HepG2 cancer cell lines. Thus, the potential of the complex 1 is promising though further in vivo investigations may be required before subjecting it to clinical trials

Effect of Ambient Gases on Respiration of Soil Supporting Four Crops in Central Saudi Arabia

This study was conducted at four localities (Maseef, Naseem, Oleya and Industrial City) in Riyadh city, KSA to determine the effect of increased tropospheric gases on responses of in situ soil respiration (Rs) of wheat (Triticum aestivum L. cv. Giza 68), broad bean (Vicia faba L. cv. Lara), kidney bean (Phaseolus vulgaris L. cv. Giza 3) and pea (Pisum sativum L. cv. Perfection) rhizosphere soil. These plants were grown to a full-season in pots to recieve four air quality localities treatments. Daily mean of O3, SO2, NO2 and CO2 concentrations were recorded by portable gas analyzers in the center of studied localities. The Rs values were measured monthly before seed germination, during all growth stages and after harvesting (October, December, February, April and June) at three times during the day (morning, noon and afternoon) for each stage. The maximum values recorded for O3 in mid June, 2007 were 39, 77, 95 and 166 nL L-1, in Maseef, Naseem, Olea and Industrial City localities, respectively. Significant decreases in Rs were observed for all polluted localities in compared Maseef site (less polluted). The greatest decreases in Rs were found at Industrial City followed by Naseem and Oleya. More reductions in Rs were observed for the Industrial City treatments during flowering and grainfill stages, while normal respiration at Maseef area was recorded. This study concluded that O3 injury can reduce the Rs by decreasing the activities and reactions in soil supporting plants

Patient's desire and preference for provision of information toward greater involvement in shared care

Objectives: To determine the perceptions of patients on whether they receive sufficient information about their medical problems, their preferences to obtain information, and factors that may influence their preferences. Design and Settings: Cross-sectional, questionnaire-based study conducted in a primary health-care center affiliated with the National Guard Hospital, Riyadh, Saudi Arabia. Patients and Methods: Patients attending the center between October and December 2010 were interviewed using a questionnaire developed to meet the objectives of the study. Results: A total of 245 patients participated in the study. The mean (±standard deviation) age of the participants was 43 (±16) years. Reported cases of dyslipidemia, diabetes mellitus, and hypertension among participants were 42%, 39%, and 31%, respectively. A minority of the participants indicated that they had a sufficient knowledge of their medical problems. The vast majority of the patients (92%) indicated that their preference to be informed about available treatment options and the plan for their future treatment. However, only 38% indicated that they had been told about the available treatment options, and less than half (48%) were informed about their future treatment plan. The proportion of male patients who preferred to know the treatment plan for their medical problems was significantly more than that of females (P < 0.001); nevertheless, female participants perceived that they had been better informed about their treatment plan than the male participants (P = 0.003). Conclusion: This study demonstrates that patients receive information about their medical problems much less than their expectations. Measures to promote patient education and their involvement in shared care process should be considered and implemented to minimize serious health outcomes

Pollen morphology of certain species of the family Lamiaceae in Saudi Arabia

The intention of the present wok is to provide an account on the pollen morphological features of Lamiaceae in Saudi Arabia as a basis for future studies of Lamiaceae pollens in the region. Pollen morphology of 20 species belong to 16 genera of the Lamiaceae has been investigated using Light Microscope (LM) and Scanning Electron Microscope (SEM). The study revealed that the pollen grains were characterized by 3-zonocolpate or 6-zonocolpate. Size of the pollen is variable between the genera but not among the species of the same genus. The surface pattern of the exine varies from fine reticulate, rough reticulate, mega-reticulate, reticulate-perforate, bireticulate-perforate or granulate, leading to 6 types of pollen grains. These variations revealed by this study implies that pollen Morphology may be of significant value sharing in solving problems in the classification of Lamiaceae members. A Key to the species, based on the morphological features of pollen grains, is also provided

Tropospheric ozone effects on the productivity of some crops in central Saudi Arabia

This study was conducted to evaluate damaging degree of ambient ozone (O3) levels in certain economically important crops in typical areas of the central KSA (Riyadh). Daily mean ozone concentrations were recorded by portable O3 analyzers in the center of Batha, Naseem, Oleya and Industrial City, from the beginning of October, 2006 to middle of June, 2007. Maseef area was used as control because it is receiving fewer pollutants (O3 levels less than 40 nL L-1). Selected crops grown in pots were exposed to short-term of pollution at defined localities. These crops include Triticum aestivum L. cv. Giza 68 (wheat), Vicia faba L. cv. Lara, (broad bean), Phaseolus vulgaris L. cv. Giza 3 (kidney bean) and Pisum sativum L. cv. Perfection (pea). The exposure indicators of them are length, injury symptoms, biomass and yield. The maximum values of daily O3 were 125 nL L-1, 77 nL L-1, 95 nL L-1 and 166 nL L-1, in all the four studied areas, respectively in mid June, 2007. Results showed that the estimated yield losses varied in all four studied areas, being 35, 9, 39 and 46%, respectively for wheat; being 16, 13, 21 and 33%, respectively for broad bean; being 22, 20, 28 and 45%, respectively for kidney bean and being 5, 3, 14 and 30%, respectively for pea. This research recommended that these plant species can be used to give bio-indicator significance to assess ambient ozone impacts of different examined areas in KS