Dietary and genetic factors associated with risk for development of colorectal cancer: Case-control study in a Basque population

207 p.To date, case-control studies have revealed inconsistent evidence on the influence of dietary and genetic factors on colorectal cancer (CRC) risk. In order to better elucidate the role of some of these factors in the aetiology of CRC, the main objective of this study was to analyse dietary and genetic factors in a sample of cases and controls from the population-based CRC screening programme of the Osakidetza/Basque Health Service. In addition, taking into account that an unhealthy diet is associated with the risk of tumour recurrence, metastasis and death, the other aim of this thesis was to assess the adequacy of nutrients consumed and diet quality in a group of CRC patients postsurgery. The results showed that the diet of the studied CRC patients postsurgery is inadequate in many respects, including nutrients and food intakes. In fact, this inadequacy is associated with certain health determinants. On the other hand, there are direct associations between CRC risk and high-fat cheese, and inverse associations with fibre containing foods and fatty fish, as well as adherence to a Mediterranean Diet pattern, in the case-control sample analysed. With respect to genetic factors, it was confirmed a CRC susceptibility locus and the existence of associations between modifiable factors and the rs6687758 SNP; moreover, the Genetic Risk Score was associated with CRC. However, further studies are needed to better understand the influence of the dietary habits on CRC prevention and to establish the role of the genetic factors, as well as the contribution of the gene-diet interactions to the risk of CRC in this population

Role of Dairy Foods, Fish, White Meat, and Eggs in the Prevention of Colorectal Cancer: A Systematic Review of Observational Studies in 2018–2022

[EN] There is limited evidence to support the relationship between the consumption of animal-source foods other than red meat and processed meat and colorectal cancer (CRC) risk. We aimed to examine the recent available evidence from observational studies about the association between these food groups’ intake and CRC risk. For this systematic review, we searched the PubMed database for the last five years. A total of fourteen cohort studies and seven case–control studies comprising a total of >60,000 cases were included. The studies showed a consistent significant decrease in CRC risk, overall and by subsites, associated with a high consumption of total dairy products. Less strong effects associated with the consumption of any subtype of dairy product were observed. Fish consumption, overall and by subtypes (oily or non-oily and fresh or canned), showed a mild inverse association with CRC risk. The association between white meat and egg intake and CRC risk was low and based on a small number of studies; thus, these findings should be interpreted with caution. In conclusion, a high consumption of total dairy products was associated with a lower CRC risk. However, evidence for fish, white meat, and eggs and the CRC risk were not as strong.This research received no external funding. CIBERehd is funded by the Instituto de Salud Carlos III. BIOMICS Research Group, Microfluidics & BIOMICS Cluster of the UPV/EHU is funded by the Basque Government. Funding of article processing charges provided by the Spanish Gastroenterology Foundation

Relationship between the ratio of fish to meat consumption and diet adequacy and quality in university young women

9 p.[ES] Introducción: En las poblaciones occidentales el consumo de pescado es menor que el de carnes y esto puede influir en la calidad de la dieta total. Objetivo: Comprobar si la relación entre el consumo de pescado y carne está vinculada con la adecuación y calidad de la dieta en un grupo de mujeres universitarias. Participantes y métodos: En este estudio participaron un total de 47 mujeres de la Universidad del País Vasco (UPV/EHU) con una edad media fue de 19,6+/-1,2 años. La ingesta dietética se evaluó con un cuestionario de frecuencias de consumo validado. A partir de los datos cuantitativos de consumo se calculó la ingesta de energía y nutrientes, utilizando el programa CESNID. Se estimaron dos índices de calidad de la dieta: el índice pescado/carne y el Índice de Alimentación Saludable para población española (IASE). El análisis estadístico se realizó con el programa SPSS versión 21.0. Resultados: El índice pescado/carne fue de 0,4 y la mayoría de las participantes (91,5%) consumían más carnes que pescado. Las universitarias que tenían mayores ingestas de pescado consumían más fruta y menos dulces. El índice pescado/carne se asoció positivamente con mayores puntuaciones en muchos de los componentes del IASE y con mayor adecuación en la ingesta de algunos nutrientes: proteínas, niacina, potasio, hierro, zinc, vitamina D, E, tiamina, riboflavina, piridoxina, vitamina B12 y C. Conclusión: Un mayor consumo de pescado que de carne está relacionado con un mejor perfil dietético en el consumo de ciertos alimentos y nutrientes, en la muestra estudiada.[EN] Introduction: In Western populations fish consumption is lower than meat consumption and this fact can influence on the total diet quality. Objective: Verify if the relationship between the ratio of fish to meat consumption and diet adequacy and quality in a group of women university students. Participants and methods: 47 women of the University of the Basque Country (UPV/EHU) took part in this study; the mean age was 19.6+/-1.2 years. Dietary intake was evaluated with a food frequency questionnaire validated. From quantitative data on food, energy and nutrients intake were estimated by CESNID software. Two diet quality indices were also calculated: the ratio of fish to meat consumption and the Healthy Eating Index for Spanish diet (HEISD). Statistical analyses were carried out using the SPSS 21.0 software package. Results: The ratio of fish to meat was 0.4 and the majority of the participants (91.5%) eaten more meat than fish. The university students with a high intake of fish were eating more fruit and less sweets. The ratio fish/meat was associated positively with the scores of some components of the HEISD and with the intake of some nutrients: proteins, niacin, potassium, iron, zinc, vitamin D, E, thiamine, riboflavin, pyridoxine, vitamin B12 and C. Conclusion: A higher consumption of fish than meat was associated with a better dietary profile in the consumption of some foods and nutrients, in the sample studied

Single nucleotide polymorphisms associated with susceptibility for development of colorectal cancer: Case-control study in a Basque population

Given the significant population diversity in genetic variation, we aimed to investigate whether single nucleotide polymorphisms (SNPs) previously identified in studies of colorectal cancer (CRC) susceptibility were also relevant to the population of the Basque Country (North of Spain). We genotyped 230 CRC cases and 230 healthy controls for 48 previously reported CRC-susceptibility SNPs. Only the rs6687758 in DUPS10 exhibited a statistically significant association with CRC risk based on the crude analysis. The rs6687758 AG genotype conferred about 2.13-fold increased risk for CRC compared to the AA genotype. Moreover, we found significant associations in cases between smoking status, physical activity, and the rs6687758 SNP. The results of a Genetic Risk Score (GRS) showed that the risk alleles were more frequent in cases than controls and the score was associated with CRC in crude analysis. In conclusion, we have confirmed a CRC susceptibility locus and the existence of associations between modifiable factors and the rs6687758 SNP; moreover, the GRS was associated with CRC. However, further experimental validations are needed to establish the role of this SNP, the function of the gene identified, as well as the contribution of the interaction between environmental factors and this locusto the risk of CRC.This work was supported by two projects (from the Department of Health and Consumer Affairs, Basque Government 2011111153; and Saiotek, Basque Government S-PE12UN058), by a pre-doctoral grant from the Basque Government (PRE_2016_2_0046), by the CIBERehd and by the U.S. Department of Agriculture-Agricultural Research Service (ARS), under agreement. 581950-4-003. Neither Basque Government nor U.S. Department of Agriculture-Agricultural Research Service (ARS) had a role in the design, analysis or writing of this article. CIBERehd is funded by the Instituto de Salud Carlos III

Food groups, diet quality and colorectal cancer risk in the Basque Country

BACKGROUND The results obtained to date concerning food groups, diet quality and colorectal cancer (CRC) risk vary according to criteria used and the study populations. AIM To study the relationships between food groups, diet quality and CRC risk, in an adult population of the Basque Country (North of Spain). METHODS This observational study included 308 patients diagnosed with CRC and 308 ageand sex-matched subjects as controls. During recruitment, dietary, anthropometric, lifestyle, socioeconomic, demographic and health status information was collected. Adherence to the dietary recommendations was evaluated utilizing the Healthy Eating Index for the Spanish Diet and the MedDietScore. Conditional logistic regressions were used to evaluate the associations of food group intakes, diet quality scores, categorized in tertiles, with CRC risk. RESULTS The adjusted models for potential confounding factors showed a direct association between milk and dairy products consumption, in particular high-fat cheeses [odds ratio (OR) third tertile vs first tertile = 1.87, 95% confidence intervals (CI): 1.11-3.16], and CRC risk. While the consumption of fiber-containing foods, especially whole grains (OR third tertile vs first tertile = 0.62, 95%CI: 0.39-0.98), and fatty fish (OR third tertile vs first tertile = 0.53, 95%CI: 0.27-0.99) was associated with a lower risk for CRC. Moreover, higher MD adherence was associated with a reduced CRC risk in adjusted models (OR third tertile vs first tertile = 0.40, 95%CI: 0.20-0.80). CONCLUSION Direct associations were found for high-fat cheese, whereas an inverse relation was reported for fiber-containing foods and fatty fish, as well as adherence to a Mediterranean dietary pattern.Supported by the Department of Health and Consumer Affairs, Basque Government, No. 2011111153; Saiotek, Basque Government, No. S-PE12UN058; Pre-doctoral grant from the Basque Government, No. PRE_2015_2_0084; and United States Department of Agriculture—Agricultural Research Service, No. 58-1950-4-003

Gene–Diet Interactions in Colorectal Cancer: Survey Design, Instruments, Participants and Descriptive Data of a Case–Control Study in the Basque Country

Epidemiologic studies have revealed inconsistent evidence of gene-diet interaction in relation to colorectal cancer (CRC). The aim of this study was to analyze them in a sample of cases and controls from the population-based bowel cancer screening program of the Osakidetza/Basque Health Service. This study analyzed dietetic, genetic, demographic, socioeconomic factors and lifestyles. In the present manuscript, the survey design, sampling, instruments, measurements and related quality management were presented. Moreover, we analyze di erences between cases and controls in some data, especially those related to diet. The participants were 308 cases and 308 age- and sex-matched subjects as controls. Cases were more likely than controls to have overweight/obesity (67.5% vs. 58.1%, p < 0.05), a lower intake of vitamin B2 (0.86 0.23 vs. 0.92 0.23 mg/1000 kcal, p < 0.01) and calcium:phosphorus ratio (0.62 0.12 vs. 0.65 0.13, p < 0.01). A higher proportion of cases than controls did not meet the Nutritional Objectives for saturated fatty acids (85.7% vs. 67.5%, p < 0.001) or cholesterol (35.4% vs. 25.0%, p < 0.01). In conclusion, the present study provides valuable data for analyzing the complexity of gene-diet interaction in relation to CRC. The results presented here suggest that overweight/obesity and a high intake of certain dietary components, especially saturated fatty acids and cholesterol, are more frequent in cases than in controls.This research was supported by the Department of Health and Consumer A airs of the Basque Government (2011111153) and Saiotek program of the Basque Government (S-PE12UN058). I.A.-L. was founded by a pre-doctoral grant from the Basque Government (PRE_2014_1_161, PRE_2015_2_0084, EP_2016_1_0098, EP_2016_1_0098 and PRE_2017_2_0006). The U.S. Department of Agriculture—Agricultural Research Service (ARS), under Agreement No. 58-1950-4-003. CIBERehd is funded by the Instituto de Salud Carlos III

Cheese consumption and prevalence of overweight and obesity in a Basque adult population: a crosssectional study

[EN] Studies have reported a negative association between dairy product consumption and weight status. However, not as much research has focused on cheese; therefore, the aim of this study was to study the association between cheese intake and overweight and obesity in a representative Basque adult population. A food frequency questionnaire (FFQ) was obtained from a random sample of 1081 adults (530 males and 551 females, 17–96 years old). Cheese consumption data were expressed as g/1000 kcal/day. The prevalence of overweight/obesity was higher in men (55.1%) than in women (35.4%) (p50.001). Participants with low or moderate intake of fresh and processed cheese demonstrated a higher prevalence of excess weight, compared with those with higher consumption. The confounding variables selected in multivariate analysis were occupational status and age in both genders; and place of residence in men. In conclusion, negative associations were found between consumption of some types of cheese and overweight and obesity in this population

Relationship between the ratio of fish to meat consumption and diet adequacy and quality in university young women

9 p.[ES] Introducción: En las poblaciones occidentales el consumo de pescado es menor que el de carnes y esto puede influir en la calidad de la dieta total. Objetivo: Comprobar si la relación entre el consumo de pescado y carne está vinculada con la adecuación y calidad de la dieta en un grupo de mujeres universitarias. Participantes y métodos: En este estudio participaron un total de 47 mujeres de la Universidad del País Vasco (UPV/EHU) con una edad media fue de 19,6+/-1,2 años. La ingesta dietética se evaluó con un cuestionario de frecuencias de consumo validado. A partir de los datos cuantitativos de consumo se calculó la ingesta de energía y nutrientes, utilizando el programa CESNID. Se estimaron dos índices de calidad de la dieta: el índice pescado/carne y el Índice de Alimentación Saludable para población española (IASE). El análisis estadístico se realizó con el programa SPSS versión 21.0. Resultados: El índice pescado/carne fue de 0,4 y la mayoría de las participantes (91,5%) consumían más carnes que pescado. Las universitarias que tenían mayores ingestas de pescado consumían más fruta y menos dulces. El índice pescado/carne se asoció positivamente con mayores puntuaciones en muchos de los componentes del IASE y con mayor adecuación en la ingesta de algunos nutrientes: proteínas, niacina, potasio, hierro, zinc, vitamina D, E, tiamina, riboflavina, piridoxina, vitamina B12 y C. Conclusión: Un mayor consumo de pescado que de carne está relacionado con un mejor perfil dietético en el consumo de ciertos alimentos y nutrientes, en la muestra estudiada.[EN] Introduction: In Western populations fish consumption is lower than meat consumption and this fact can influence on the total diet quality. Objective: Verify if the relationship between the ratio of fish to meat consumption and diet adequacy and quality in a group of women university students. Participants and methods: 47 women of the University of the Basque Country (UPV/EHU) took part in this study; the mean age was 19.6+/-1.2 years. Dietary intake was evaluated with a food frequency questionnaire validated. From quantitative data on food, energy and nutrients intake were estimated by CESNID software. Two diet quality indices were also calculated: the ratio of fish to meat consumption and the Healthy Eating Index for Spanish diet (HEISD). Statistical analyses were carried out using the SPSS 21.0 software package. Results: The ratio of fish to meat was 0.4 and the majority of the participants (91.5%) eaten more meat than fish. The university students with a high intake of fish were eating more fruit and less sweets. The ratio fish/meat was associated positively with the scores of some components of the HEISD and with the intake of some nutrients: proteins, niacin, potassium, iron, zinc, vitamin D, E, thiamine, riboflavin, pyridoxine, vitamin B12 and C. Conclusion: A higher consumption of fish than meat was associated with a better dietary profile in the consumption of some foods and nutrients, in the sample studied

Association between nutrient intake related to the one-carbon metabolism and colorectal cancer risk: a case–control study in the Basque Country

Purpose Epidemiologic evidence for the association between methyl-donor nutrient intake and colorectal cancer (CRC) risk remains inconclusive. We aimed to examine the relationship between intake of vitamins of the B group, methionine, total choline and betaine and CRC risk, in a population from the CRC screening programme in the Basque Country. Design This observational study included 308 patients with CRC and 308 age- and sex-matched subjects as controls. During recruitment, dietary, anthropometric, lifestyle, socioeconomic, demographic, and health status information was collected. Conditional logistic regression was used to estimate the odds ratios (ORs) for CRC risk. Results The adjusted ORs for CRC risk decreased with higher intakes of choline and betaine (p < 0.05). After further adjustment for folate, high intake of choline and betaine remained associated with a reduced CRC risk (adjusted model for choline, OR third tertile vs first tertile = 0.45, 95% CI 0.26–0.80, p = 0.006; for betaine, OR third tertile vs first tertile = 0.27, 95% CI 0.16–0.47, p < 0.001). Regarding the other nutrients, our findings indicated a non-significant decrease in CRC risk with the high level of intake. Conclusions Our data suggest that choline and betaine intake influence CRC risk in the studied population.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work was supported by the Basque Government (Department of Health and Consumer Affairs, No. 2011111153; Saiotek programme, No. S-PE12UN058; BIOMICs Research Group, Microfluidics & BIOMICs Cluster of the University of the Basque Country UPV/EHU, No. IT1633-22). I.A.-L. was founded by a pre-doctoral grant from the Basque Government (PRE_2014_1_161, PRE_2015_2_0084, EP_2016_1_0098, EP_2016_1_0098, and PRE_2017_2_0006)

Cheese consumption and prevalence of overweight and obesity in a Basque adult population: a cross-sectional study

[EN] Studies have reported a negative association between dairy product consumption and weight status. However, not as much research has focused on cheese; therefore, the aim of this study was to study the association between cheese intake and overweight and obesity in a representative Basque adult population. A food frequency questionnaire (FFQ) was obtained from a random sample of 1081 adults (530 males and 551 females, 17–96 years old). Cheese consumption data were expressed as g/1000 kcal/day. The prevalence of overweight/obesity was higher in men (55.1%) than in women (35.4%) (p50.001). Participants with low or moderate intake of fresh and processed cheese demonstrated a higher prevalence of excess weight, compared with those with higher consumption. The confounding variables selected in multivariate analysis were occupational status and age in both genders; and place of residence in men. In conclusion, negative associations were found between consumption of some types of cheese and overweight and obesity in this population