Nova tecnologia de poda para o café Arábica: poda programada de ciclo para o café Arábica

This study aimed to better understand the productive behavior of Coffea arabica when cultivated using the new management of Cyclic Pruning Program for Arabica Coffee (CPPAC), stablished using the same principles of the Cyclic Pruning Program for Conilon Coffee. The experiment was carried out in Baixo Guandú, Espírito Santo state, in the Southeast region of Brazil, in order to test the conditioning of this new pruning management for Arabica coffee (CPPAC) over the crop yield of a plantation of Catuaí Vermelho IAC 81. It was found increased crop yield with use of the (CPPAC) over the traditional management of Arabica coffee, considering five consecutive years of production. The (CPPAC) technique can be used as a viable alternative for pruning management of Arabica coffee.Este estudo teve como objetivo compreender melhor o comportamento produtivo do café, Coffea Arábica quando cultivado usando a nova tecnologia da Poda Programa de Ciclo para o Café Arábica (PPCA), estabelecida usando os mesmos princípios da Poda Programa de Ciclo do Café Conilon. O experimento foi desenvolvido em Baixo Guandu, no Espírito Santo, na região Sudeste do Brasil, a fim de testar o condicionamento dessa nova tecnologia de poda para o Café Arábica (PPCA) sobre o rendimento da colheita de uma plantação de café Catuaí Vermelho IAC 81. Foi encontrado aumento da produtividade com o uso da PPCA sobre a poda tradicional do café arábica, considerando cinco anos consecutivos de produção. A técnica da PPCA pode ser usada como uma alternativa viável para a poda do café arábica

Concentrações de microminerais (cobre, cobalto, ferro, molibdênio e zinco) no fígado de vacas leiteiras da Região de Campos Gerais, Paraná, Brasil

Universidade Federal do Paraná. Departamento de Medicina Veterinária. Curitiba, PR, Brazil.Universidade Federal do Pará - Campus Castanhal. Instituto de Medicina Veterinária. Castanhal, PA, Brazil.Universidade Federal do Pará - Campus Castanhal. Instituto de Medicina Veterinária. Castanhal, PA, Brazil.Universidade Federal do Sul e Sudeste do Pará. Instituto de Estudos do Trópico Úmido. Xinguara, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Laboratório de Toxicologia. Ananindeua, PA, Brasil.Universidade Federal do Paraná. Departamento de Medicina Veterinária. Curitiba, PR, Brazil.Universidade do Estado de Santa Catarina. Departamento de Medicina Veterinária. Florianópolis, SC, Brazil.Universidade Federal do Paraná. Departamento de Medicina Veterinária. Curitiba, PR, Brazil.Universidade Federal do Paraná. Departamento de Medicina Veterinária. Curitiba, PR, Brazil.This study collected samples from 50 Holstein cows, most intensively bred, and from the Campos Gerais region, Paraná, with an average milk production of 30.21L/day. Samples of the liver, spleen and lymph nodes were collected to determine the levels of copper, cobalt, iron, molybdenum and zinc. Spleen and lymph nodes were subjected to histological analysis and evaluation of the degree of hemosiderosis. The average concentrations of copper (495.05ppm), molybdenum (4.19ppm), and zinc (274.49ppm) were higher than those established for the bovines. For cobalt 26% of the animals presented levels below the established level, which characterized cobalt deficiency. Only iron (299.12ppm) exhibited an adequate average level. Histopathologically hemosiderosis was observed mainly in the spleen (78%) and less frequently in the lymph nodes (20%). The observation of hemosiderin in the spleen and lymph nodes is not related to copper deficiency. Still it may be related to high levels of molybdenum, zinc, iron, or other undetermined causes

Influence of fertilization on nitrate content in pastures and milk in the west of Santa Catarina over the year

The indiscriminate use without criteria of nitrogen fertilization can lead to an accumulation of nitrate in pastures, animal poisoning and potential increase in residual content of this compound in milk. The objective of this study was to determine the residual levels of nitrate throughout the year in pastures and milk from small farms in western Santa Catarina (SC) that use high levels of nitrogen fertilization. The experiment was carried out from April/2018 to March/2019 on 10 dairy farms in the municipality of Riqueza (SC). Two annual collections of water samples and monthly collections of pastures and milk were carried out, in addition to obtaining information on the property and management of pasture fertilization through a structured questionnaire. Nitrate was also evaluated in pastures using the diphenylamine test. Temperature and rainfall data were obtained daily by a weather station. The average amount of nitrogen fertilizer used in the properties was 654 ± 176 kg/ha/year. The average nitrate content in the drinking water of the animals was 1.5 ± 1.4 mg/L, in the pasture it was 270 ± 76 mg/kg DM, while in milk it was 2.0 ± 0.3 mg/L. There was seasonal variation with an increase in nitrate content in pastures and milk in autumn, a period of the experiment in which low rainfall was observed. It was concluded that despite being dairy properties with high use of nitrogen fertilization, safe milk is produced in terms of nitrate levels, even in times of the year with adverse climatic conditions. The diphenylamine test has a good ability to discriminate the nitrate content in pastures and can be indicated as a quick test to verify the presence of high levels of nitrate in the pasture.O uso indiscriminado da adubação nitrogenada pode levar ao acúmulo de nitrato nas pastagens, intoxicar animais e aumentar os níveis nocivos residuais no leite destinado ao consumo humano. Dessa forma, o objetivo desse estudo foi determinar os teores de nitrato nas pastagens e no leite ao longo de um ano em propriedades que utilizam níveis elevados de adubação nitrogenada no oeste de Santa Catarina (SC). O experimento foi realizado durante o período de abril/2018 a março/2019 em dez propriedades produtoras de leite do município de Riqueza (SC). Foram realizadas duas coletas anuais de amostras de água e coletas mensais de pastagens e leite, além de obtidas informações quanto ao manejo de adubação das pastagens da propriedade. O teste de difenilamina foi realizado nas pastagens. Dados de temperatura e pluviometria foram obtidos diariamente por uma estação meteorológica. A quantidade média de adubo nitrogenado utilizado nas propriedades foi de 654 ± 176 kg/ha/ano. O teor médio de nitrato na água de consumo dos animais foi de 1,5 ± 1,4 mg/L, na pastagem foi de 270 ± 76 mg/kg MS, enquanto no leite foi de 2,0 ± 0,3mg/L. Houve variação sazonal com aumento no teor de nitrato nas pastagens e no leite no outono, período do experimento em que foi observado baixo índice pluviométrico. Concluiu-se que apesar de se tratar de propriedades leiteiras com elevada utilização de adubação nitrogenada, o leite produzido é seguro quanto aos teores de nitrato, mesmo em épocas do ano com condições climáticas adversas. O teste de difenilamina pode ser indicado como teste rápido para verificar teores elevados de nitrato na pastagem

Lesions in the skin of cattle associated to hairy vetch consumption (Vicia villosa)

Background: Hairy vetch (Vicia spp.) is a high-protein source forage to cattle. The poisoning is clinically characterized by a systemic granulomatous disease, which causes dermatitis, diarrhea, decreased milk production and weight loss. The specie of hairy vetch related to systemic granulomatous disease in cattle is Vicia villosa. This work aims to describe the epidemiological, clinical, gross, microscopic and immunohistochemistry features of the skin lesions caused by the consumption of V. villosa in cattle affected by the systemic granulomatous disease. Materials, Methods & Results: A retrospective study of necropsy and biopsy exams performed between the period of 2005-2016 aiming for cattle with systemic granulomatous disease after consumption of hairy vetch was carried out in the archives of the Setor de Patologia Veterinária from the UFRGS. Epidemiological data included the sex, age, and breed of the animals affected. Gross and microscopical lesions, in addition to the immunohistochemistry anti-T lymphocytes (CD3), anti-B lymphocytes (CD79a), and anti-macrophages (CD68) features, were evaluated. The histological lesions and immunohistochemistry staining were quantified in mild (+), moderate (++), and severe (+++). The diagnosis of systemic granulomatous disease with skin lesions after consumption of Vicia villosa was observed in eight cattle. All animals were females, with 5-8 year-old (average 6.6 years), Holstein Friesian cattle (7) and Jersey (1) breeds. These cattle had a clinical history of severe pruritus, anorexia, apathy, decreased milk production, weight loss, and hyperthermia. Grossly, lesions were characterized by alopecia (8/8), crusts (7/8), lichenification and seborrhea (2/8), and exudative lesions (2/8), and involved the head (7/8), limbs (5/8), neck (4/8), trunk (4/8), perineum area (3/8), udder (3/8), and tail (3/8) Histology revealed a mild to moderate inflammatory infiltrate (7/7), composed by lymphocytes (7/7), macrophages (7/7), occasional eosinophils, and rare multinucleated giant cells (1/7). It was also classified in mild to severe perivascular dermatitis (7/7), mild to moderate perifolliculitis (4/7), superficial dermatitis (3/7), moderate to severe mural folliculitis (2/7), and hidradenitis (1/7). Another findings were moderate apocrine sweat gland ectasia, mild to moderate orthokeratotic hyperkeratosis, mild to moderate spongiosis, mild to moderate acanthosis, mild to severe serocellular crusts, mild pigmentary incontinence, mild to severe Munro’s microabscesses, mild to moderate hydropic degeneration, apoptosis, ulcers, mild to moderate superficial edema of the dermis, variable epitheliotropism and erosions. Immunohistochemistry of all skin sections had mild to severe staining for T cell (CD3), mild staining for macrophages (CD68), and immunostaining was rare (4/7) or absent (3/7) for B cells (CD79a). Discussion: Cutaneous lesions observed in this study were similar to those previously described by other authors for this condition, and were characterized by focal to coalescent areas of alopecia, lichenification and seborrhea, associated clinically to a severe pruritus. Histologically, these consisted of perivascular dermatitis, and perifolliculitis with an inflammatory infiltrate composed predominantly by lymphocytes, macrophages, with occasional eosinophils and multinucleated giant cells. Immunohistochemistry demonstrates a marked immunostaining for T cells, while it was mild for macrophages, which reinforces the involvement of the delayed type hypersensitivity (type IV) reaction in the pathogenesis of the disease