843 research outputs found
Kellyās story: transformative identity work in primary mathematics teacher education
This article explores one female student teacherās experiences of
learning to teach mathematics. Data included weekly emails and
an interview through which Kelly expressed her struggle to be
recognised as mathematical and to be heard in pedagogical
relationships within a subject that is discursively aligned with
masculinity. Analysis drew on feminist post-structural
understandings of discourse and power relations. I identify an
array of discourses of gender, mathematics, ability, confidence
and complex micro-relations of power which shaped her āidentity
workā and examine how she was able to reposition herself
positively as a teacher of mathematics, resisting dominant
transmission-based approaches to teaching mathematics. While
the course sought to challenge traditional mathematical
pedagogies, gendered ways of being mathematical were not
confronted. I argue that teacher educators need to pay attention
to the way power circulates to marginalise and silence some
students and to focus on transformation of their own identity work
Zebrafish: An in vivo model for the study of neurological diseases
As the population ages, there is a growing need for effective therapies for the treatment of neurological diseases. A limited number of therapeutics are currently available to improve cognitive function and research is limited by the need for in vivo models. Zebrafish have recently become a focus of neurobehavioral studies since larvae display neuropathological and behavioral phenotypes that are quantifiable and relate to those seen in man. Due to the small size of Zebrafish larvae, assays can be undertaken in 96 well plates and as the larvae can live in as little as 200 Ī¼l of fluid, only a few milligrams of compound are needed for screening. Thus in vivo analysis of the effects of compounds can be undertaken at much earlier stages in the drug discovery process. This review will look at the utility of the zebrafish in the study of neurological diseases and its role in improving the throughput of candidate compounds in in vivo screens
Pre service primary teachersā approaches to mathematical generalisation
In our teaching with primary pre-service teachers (PSTs), each of us includes generalising tasks in the context of mathematical reasoning. We set out to explore the value of such activity from the perspective of PSTs and their approaches to generalisation. In this paper, we focus on one PSTās mathematical reasoning when working on the āflower bedsā problem. We analyse the ways in which this PST attends to: looking for a relationship; seeing structure within a single figure in a sequence; and seeing sameness and difference between figures in a sequence. We consider what motivates shifts in attention, we reflect on the significance of studentsā prior experience, and of student collaboration in our teaching sessions
Structure of the PII signal transduction protein of Neisseria meningitidis at 1.85ā Ć resolution
The structure of the PII signal transduction protein of N. meningitidis at 1.85ā
Ć
resolution is described
A versatile ligation-independent cloning method suitable for high-throughput expression screening applications
This article describes the construction of a set of versatile expression vectors based on the In-Fusionā¢ cloning enzyme and their use for high-throughput cloning and expression screening. Modifications to commonly used vectors rendering them compatible with In-Fusionā¢ has produced a ligation-independent cloning system that is (1) insert sequence independent (2) capable of cloning large PCR fragments (3) efficient over a wide (20-fold) insert concentration range and (4) applicable to expression in multiple hosts. The system enables the precise engineering of (His6-) tagged constructs with no undesirable vector or restriction-site-derived amino acids added to the expressed protein. The use of a multiple host-enabled vector allows rapid screening in both E. coli and eukaryotic hosts (HEK293T cells and insect cell hosts, e.g. Sf9 cells). These high-throughput screening activities have prompted the development and validation of automated protocols for transfection of mammalian cells and Ni-NTA protein purification
The 14q32 maternally imprinted locus is a major source of longitudinally stable circulating microRNAs as measured by small RNA sequencing
Understanding the normal temporal variation of serum molecules is a critical factor for identifying useful candidate biomarkers for the diagnosis and prognosis of chronic disease. Using small RNA sequencing in a longitudinal study of 66 women with no history of cancer, we determined the distribution and dynamics (via intraclass correlation coefficients, ICCs) of the miRNA profile over 3 time points sampled across 2ā5 years in the course of the screening trial, UKCTOCS. We were able to define a subset of longitudinally stable miRNAs (ICC >0.75) that were individually discriminating of women who had no cancer over the study period. These miRNAs were dominated by those originating from the C14MC cluster that is subject to maternal imprinting. This assessment was not significantly affected by common confounders such as age, BMI or time to centrifugation nor alternative methods to data normalisation. Our analysis provides important benchmark data supporting the development of miRNA biomarkers for the impact of life-course exposure as well as diagnosis and prognostication of chronic disease
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