Poly-extremophiles: exploring the limits of habitability

Brevibacterium linens AE038-8 is an arsenic hyper-tolerant bacterial strain, previously isolated from well water in Tucumán, Argentina. The aim of this study is to investigate the resistance of this strain to different stress factors relevant for astrobiology studies. We found that B. linens AE038-8 is capable of tolerating high concentrations of heavy metals such as Cd(II), Cr(VI) and Cu(II), and a range of temperatures from 10 to 30°C. It is also able to grow in the range of pH values from 3 to 11, and when grown in the presence of NaCl, it can tolerate concentrations up to 5 M. We also study the ability of the strain to grow under combined stress conditions. In particular, we found that it is able to grow under high NaCl concentrations and low pH, conditions relevant to the habitability of brines in Mars.Fil: Maizel, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Alche, Laura Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Mauas, Pablo Jacobo David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; Argentin

Systematic and Phylogenetic Analysis of the Ole e 1 Pollen Protein Family Members in Plants

16 páginas, 2 figuras, 1 tabla.Support of the Spanish Ministry of Science and Innovation (ERDF-cofinanced project BFU2008-00629) and Andalusian Regional Government (ERDF-cofinanced Proyectos de Excelencia CVI5767 and AGR6274) is gratefully acknowledged.Peer reviewe

Likelihood of suffering from an eating disorder in a sample of Spanish cyclists and triathletes

Background: During recent years, there has been increasing interest in the study of eating disorders within sports practitioners, with prevalence being reported to be higher than in the general population. The aim of this study was to describe and predict eating disorders according to sex, body mass index, age and sport discipline within a sample of athletes. Methods: A sample of 4037 cyclists and triathletes from across Spain was selected. Athletes self-reported demographic characteristics and completed the revised restraint scale, SCOFF questionnaire and Mediterranean diet adherence screener. To be eligible for inclusion, participants had to be over eighteen years old. Results: Males were significantly less likely than females (p < 0.001; OR = 0.33), and triathletes (p < 0.01; OR = 0.76) were less likely than cyclists to suffer from an eating disorder. Possibility of suffering from an eating disorder increased with increasing body mass index (p < 0.001; OR = 1.38) and decreasing age (p < 0.001; OR = 0.97). Conclusion: Findings suggest that the roles of sex, sport discipline, age and body mass index predict risk factors for eating disorders in a sample of Spanish athletes. Clinical diagnosis seems necessary to better understand the factors and mechanisms at play when Spanish athletes develop an eating disorder. Trial registration: Ethics Committee of the University of Granada (N°883) data: 16/11/2015

Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo

Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract disease and bronchiolitis in children worldwide. No vaccine or specific, effective treatment is currently available. β-escin is one of the main bioactive constituents of Aesculus hippocastanum L. (Hippocastanaceae) seed extract (AH), and both β-escin and AH have demonstrated a beneficial role in clinical therapy because of their anti-edematous, anti-inflammatory and antioxidative effects. Besides, we have reported that β-escin and AH show virucidal, antiviral and immunomodulatory activities against the enveloped viruses HSV-1, VSV and Dengue virus in vitro. In this study, we demonstrate that β-escin and AH have virucidal and antiviral activities against RSV, as well as NF-κB, AP-1 and cytokine modulating activities in RSV infected epithelial and macrophage cell lines in vitro. Besides, in a murine model of pulmonary RSV infection, AH treatment improves the course of acute disease, evidenced by decreased weight loss, reduced RSV lung titers, and attenuated airway inflammation. In contrast, even though β-escin showed, similarly to AH, antiviral and immunomodulatory properties in vitro, it neither reduces viral titers nor attenuates lung injury in vivo. Thus, our data demonstrate that AH restrains RSV disease through antiviral and immunomodulatory effect.Fil: Salinas, Franco Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Vázquez, Luciana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; ArgentinaFil: Gentilini, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaFil: O´Donohoe, Ailin. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Histología Animal; ArgentinaFil: Regueira, Eleonora. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Histología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nabaes Jodar, Mercedes Soledad. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Viegas, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Michelini, Flavia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Hermida, Gladys Noemí. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Histología Animal; ArgentinaFil: Alche, Laura Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Bueno, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentin

First draft genome assembly of the Argane tree (Argania spinosa) [version 2; peer review: 2 approved]

BACKGROUND : The Argane tree (Argania spinosa L. Skeels) is an endemic tree of mid-western Morocco that plays an important socioeconomic and ecologic role for a dense human population in an arid zone. Several studies confirmed the importance of this species as a food and feed source and as a resource for both pharmaceutical and cosmetic compounds. Unfortunately, the argane tree ecosystem is facing significant threats from environmental changes (global warming, over-population) and over-exploitation. Limited research has been conducted, however, on argane tree genetics and genomics, which hinders its conservation and genetic improvement. METHODS : Here, we present a draft genome assembly of A. spinosa. A reliable reference genome of A. spinosa was created using a hybrid de novo assembly approach combining short and long sequencing reads. RESULTS : In total, 144 Gb Illumina HiSeq reads and 7.6 Gb PacBio reads were produced and assembled. The final draft genome comprises 75 327 scaffolds totaling 671 Mb with an N50 of 49 916 kb. The draft assembly is close to the genome size estimated by k-mers distribution and covers 89% of complete and 4.3 % of partial Arabidopsis orthologous groups in BUSCO. CONCLUSION : The A. spinosa genome will be useful for assessing biodiversity leading to efficient conservation of this endangered endemic tree. Furthermore, the genome may enable genome-assisted cultivar breeding, and provide a better understanding of important metabolic pathways and their underlying genes for both cosmetic and pharmacological.DATA AVAILABILITY: All of the A. spinosa datasets can be retrieved under BioProject accession number PRJNA294096: http://identifiers.org/ bioproject:PRJNA294096. The raw reads are available at NCBI Sequence Reads Archive under accession number SRP077839: http://identifiers.org/insdc.sra:SRP077839. The complete genome sequence assembly project has been deposited at GenBank under accession number QLOD00000000: http://identifiers. org/ncbigi/GI:1408199612. Data can also be retrieved via the International Argane Genome Consortium (IAGC) website: http://www.arganome.org.https://f1000research.compm2021BiochemistryGeneticsMicrobiology and Plant Patholog

Virest: un remedio para las conjuntivitis virales

Muchas infecciones virales de los ojos están asociadas al desarrollo de enfermedades para las cuales aun no hay vacunas y/o antivirales disponibles. El Virest y la familia de compuestos patentados comprende análogos de esteroides que presentan actividad antiviral y antiinflamatoria en una misma molécula. Este hallazgo sería de gran importancia para la prevención y/o el tratamiento de patologías de origen viral como la queratitis estromal herpética causada por el virus Herpes simplex tipo 1 y la queratoconjuntivitis epidémica debida al Adenovirus.Many ocular viral infections are associated with the development of diseases for which there are no vaccines and/or antiviral drugs available yet. Virest and the family of derived compounds patented are steroidal analogs that exhibit both antiviral and anti-inflammatory activities in the same chemical structure. This finding would be relevant to prevent and/or treat human pathologies of viral origin such as herpetic stromal keratitis caused by Herpes simplex virus type 1 and epidemic keratoconjunctivitis due to Adenoviruses.Fil: Alche, Laura Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentin

Differentiation of junin virus and antigenic variants isolated in vivo by kinetic neutralization assays

The major natural reservoir of Junin virus, the aetiological agent of Argentine haemorrhagic fever, is the cricetid Calomys musculinus. Neonatal animals experimentally infected with Junin virus (XJCl3 strain) developed typical disease and approximately 80% of them died. Most survivors become persistently infected. Antigenically variant viruses were isolated from the blood and brain of infected cricetids during the acute and chronic stages of the disease. These variants could be distinguished from the parental strain by kinetic neutralization assays using polyclonal antibodies. Some biological properties were shared with the parental virus strain including its virulence for newborn C. musculinus. These variant viruses may play a major role in chronic disease since we have shown that a viral isolate from an infected brain was poorly neutralized by serum obtained from the same animal.Fil:Alche, L.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina

Modèles probabilistes et noyaux pour l'extraction d'informations à partir de documents

PARIS-BIUSJ-Thèses (751052125) / SudocPARIS-BIUSJ-Mathématiques rech (751052111) / SudocSudocFranceF

Méthodologie d'extraction et d'analyse de réseaux de régulation de gènes (analyse de la réponse transcriptionnelle à l'irradiation chez S. cerevisiæ)

La réponse cellulaire aux dommages de l'ADN provoqués par l'irradiation (IR) est relativement bien étudiée mais de nombreuses observations montrent l'implication de l'expression de nombreux gènes. Nous souhaitons identifier les différentes formes de la réponse transcriptionnelle à l'IR et reconstruire un réseau de régulation génique impliqué dans son contrôle. La problématique réside dans l'exploitation de dynamiques d'expression de gènes dans des conditions de perturbations génétiques et dans l'intégration d'informations biologiques systémiques. Nous définissons une approche constituée d'une étape automatisée de déduction de régulations à partir de perturbations et de deux étapes d'induction qui permettent d'analyser la dynamique d'expression des gènes et d'extraire des régulations des données additionnelles. Cela nous a permis d'identifier, chez la levure, une réponse complexe à l'IR et de proposer un modèle de régulation dont certaines relations ont été validées expérimentalement.The cellular response to the DNA damage provoked by irradiation (IR) is relatively well studied, however, many observations show the involvement of the expression of many genes. We propose to identify the different potential patterns of the transcriptional response to IR and to reconstruct a gene regulatory network involved in its control. The first point of this work lies in the exploitation of the gene expression dynamics in conditions of genetic perturbations. The second point lies in the integration of systemic biological informations. We define an approach composed of one step of automated logical deduction of regulations from a strategy of perturbations and two induction steps that allow the analysis of the gene expression dynamics and the extraction of potential regulation from additional data. This approach allowed to identify, for the yeast, a complex response to IR and allowed to propose a regulation model which some relations have been experimentally validated.EVRY-Bib. électronique (912289901) / SudocSudocFranceF

Modélisation et apprentissage de systèmes et de concepts dynamiques

PARIS-BIUSJ-Thèses (751052125) / SudocPARIS-BIUSJ-Mathématiques rech (751052111) / SudocSudocFranceF