6 research outputs found
De matrimoniis inter haereticos ac inter haereticos et catholicos initis in Foederatis Belgii Provinciis : dissertationes theologicae et canonicae...
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GC-MS profiling of the phytochemical constituents of the oleoresin from Copaifera langsdorffii Desf. and a preliminary in vivo evaluation of its antipsoriatic effect.
Copaiba is the oleoresin (OR) obtained from Copaifera (Fabaceae), a neotropical tree which grows in
Amazon regions. The balsam, constituted by an essential oil and a resinous fraction is used as folkloristic
remedy in the treatment of several inflammatory diseases and for its antioxidant and antibacterial
properties. Aim of this work was (a) to carry out a characterization by GC–MS of the volatile and nonvolatile
constituents of Copaifera langsdorffii Desf. oleoresin (OR); (b) to investigate the mechanism of
its anti-inflammatory activity; (c) to evaluate its antipsoriatic effect after oral intake/topical application.
The volatile fraction (yield: 22.51%, w/w) shows: -bergamotene (48.38%), -himachalene (11.17%), -
selinene (5.00%) and -caryophyllene (5.47%). The OR residue (77.49%, w/w), after derivatization, showed
as main constituents the following compounds: copalic, abietic, daniellic, lambertinic, labd-7-en-15-oic,
pimaric, isopimaric acids and kaur16-en18-oic acid.
Preincubation of LPS-stimulated human THP-1 monocytes with increasing concentrations of the OR
purified fraction (OR-PF), containing diterpene acids, diterpenes and sesquiterpenes, reduced the release
of pro-inflammatory cytokines (IL-1, IL-6, TNF) in a dose-range of 0.1–10 M.
In addition, in cell culture system of human THP-1 monocytes, 1 M OR-PF counteracts LPS-driven
NF-B nuclear translocation.
In a preliminary clinical trial three patients affected by chronic psoriasis, treated with oral intake or
topical application of the OR, exhibited a significant improvement of the typical signs of this disease, i.e.
erythema, skin thickness, and scaliness.
In conclusion, the results of this work, beside an extensive analytical characterization of the OR chemical
composition, provide strong evidences that its anti-inflammatory activity is related to the inhibition of
the NF-B nuclear translocation, and consequently of proinflammatory cytokines secretion