Willingness-to-Pay for Improved Air Quality in Hamilton-Wentworth: A Choice Experiment

Prepared for Hamilton-Wentworth Air Quality Initiative pursuant to a memorandum of understanding among McMaster University, the Ontario Ministry of Environment and Energy and the Regional Municipality of Hamilton-Wentworth, dated November 5, 1996.

Robust magnetotelluric inversion

Author Posting. © The Author(s), 2014. This article is posted here by permission of The Royal Astronomical Society for personal use, not for redistribution. The definitive version was published in Geophysical Journal International 196 (2014): 1365-1374, doi:10.1093/gji/ggt484.A robust magnetotelluric (MT) inversion algorithm has been developed on the basis of quantile-quantile (q-q) plotting with confidence band and statistical modelling of inversion residuals for the MT response function (apparent resistivity and phase). Once outliers in the inversion residuals are detected in the q-q plot with the confidence band and the statistical modelling with the Akaike information criterion, they are excluded from the inversion data set and a subsequent inversion is implemented with the culled data set. The exclusion of outliers and the subsequent inversion is repeated until the q-q plot is substantially linear within the confidence band, outliers predicted by the statistical modelling are unchanged from the prior inversion, and the misfit statistic is unchanged at a target level. The robust inversion algorithm was applied to synthetic data generated from a simple 2-D model and observational data from a 2-D transect in southern Africa. Outliers in the synthetic data, which come from extreme values added to the synthetic responses, produced spurious features in inversion models, but were detected by the robust algorithm and excluded to retrieve the true model. An application of the robust inversion algorithm to the field data demonstrates that the method is useful for data clean-up of outliers, which could include model as well as data inconsistency (for example, inability to fit a 2-D model to a 3-D data set), during inversion and for objectively obtaining a robust and optimal model. The present statistical method is available irrespective of the dimensionality of target structures (hence 2-D and 3-D structures) and of isotropy or anisotropy, and can operate as an external process to any inversion algorithm without modifications to the inversion program.TM was supported by the scientific program of TAIGA (trans-crustal advection and in-situ reaction of global sub-seafloor aquifer) sponsored by the MEXT of Japan, and is supported by the NIPR project KP-7. ADC is supported by US National Science Foundation (NSF) grant EAR1015185

An attention-based view of short-termism:The effects of organizational structure

Debates about short-termism in business tend to center around the role of shareholder pressures and managers’ incentives, while the role of organizational structure remains understudied. In our paper, we adopt an attention-based lens to elucidate the role of organizational structure in directing the attention of management towards pressures for short-term results at the expense of the long term. Specifically, we argue that greater scale of operations, hierarchy, bureaucracy, and workforce flexibility reduce slack resources available to senior managers and increase the complexity of information presented to them in different ways. As a result, the senior managers shift their attention to short-term pressures, which are more easily understood, at the expense of attention for long-term considerations. Analysis based on a survey of senior managers in 3221 private firms in the Netherlands provides support for our arguments

Velocity-conductivity relations for cratonic lithosphere and their application : example of Southern Africa

Author Posting. © American Geophysical Union, 2013. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geochemistry, Geophysics, Geosystems 14 (2013): 806–827, doi:10.1002/ggge.20075.Seismic velocity is a function of bulk vibrational properties of the media, whereas electrical resistivity is most often a function of transport properties of an interconnected minor phase. In the absence of a minor conducting phase then the two should be inter-relatable primarily due to their sensitivity to temperature variation. We develop expressions between shear wave velocity and resistivity for varying temperature, composition, and water content based on knowledge from two kimberlite fields: Jagersfontein (Kaapvaal Craton) and Gibeon (Rehoboth Terrane). We test the expressions through comparison between a new high-resolution regional seismic model, derived from surface wave inversion of earthquake data from Africa and the surrounding regions, and a new electrical image from magnetotelluric (MT) data recorded in SAMTEX (Southern African Magnetotelluric Experiment). The data-defined robust linear regression between the two is found to be statistically identical to the laboratory-defined expression for 40 wt ppm water in olivine. Cluster analysis defines five clusters that are all geographically distinct and tectonically relate to (i) fast, cold, and variably wet Kaapvaal Craton, (ii) fast and wet central Botswana, (iii) slow, warm, and wet Rehoboth Terrane, (iv) moderately fast, cold, and very dry southernmost Angola Craton, and (v) slow, warm, and somewhat dry Damara Belt. From the linear regression expression and the MT image we obtain predicted seismic velocity at 100 km and compare it with that from seismic observations. The differences between the two demonstrate that the linear relationship between Vs and resistivity is appropriate for over 80% of Southern Africa. Finally, using the regressions for varying water content, we infer water content in olivine across Southern Africa.We wish to again acknowledge the three main funding agencies, the U.S. National Science Foundation’s Continental Dynamics Program (grant EAR0455242 to RLE), the South African Department of Science and Technology (grant to South African Council for Geoscience), and Science Foundation Ireland (grant 05/RGP/GEO001 to AGJ), for their support. Industry support for SAMTEX from De Beers Group Services, BHP Billiton and Rio Tinto Mining and Exploration resulted in a program far more extensive than originally conceived. S.F. has been supported by the NERC New Investigator grant NE/G000859/1. M.M. wishes to thank Science Foundation Ireland (grant 08/RFP/GEO1693 SAMTEX to AGJ) for support. J.F. wishes to thank Enterprise Ireland (grant Topo-Med to AGJ), Science Foundation Ireland (grant 10/IN.1/I3022 IRETHERMto AGJ), and the JAE-DOC Programme from Spanish CSIC, cofunded by FSE for support.2013-10-0

Variations in the predicted spatial distribution of atmospheric nitrogen deposition and their impact on carbon uptake by terrestrial ecosystems

Widespread mobilization of nitrogen into the atmosphere from industry, agriculture, and biomass burning and its subsequent deposition have the potential to alleviate nitrogen limitation of productivity in terrestrial ecosystems, and may contribute to enhanced terrestrial carbon uptake. To evaluate the importance of the spatial distribution of nitrogen deposition for carbon uptake and to better quantify its magnitude and uncertainty NOy-N deposition fields from five different three-dimensional chemical models, GCTM, GRANTOUR, IMAGES, MOGUNTIA, and ECHAM were used to drive NDEP, a perturbation model of terrestrial carbon uptake. Differences in atmospheric sources of NOx-N, transport, resolution, and representation of chemistry, contribute to the distinct spatial patterns of nitrogen deposition on the global land surface; these differences lead to distinct patterns of carbon uptake that vary between 0.7 and 1.3 Gt C yr−1 globally. Less than 10% of the nitrogen was deposited on forests which were most able to respond with increased carbon storage because of the wide C:N ratio of wood as well as its long lifetime. Addition of NHx-N to NOy-N deposition, increased global terrestrial carbon storage to between 1.5 and 2.0 Gt C yr−1, while the “missing terrestrial sink” is quite similar in magnitude. Thus global air pollution appears to be an important influence on the global carbon cycle. If N fertilization of the terrestrial biosphere accounts for the “missing” C sink or a substantial portion of it, we would expect significant reductions in its magnitude over the next century as terrestrial ecosystems become N saturated and O3 pollution expands

Quantum dot labeling of mesenchymal stem cells

<p>Abstract</p> <p>Background</p> <p>Mesenchymal stem cells (MSCs) are multipotent cells with the potential to differentiate into bone, cartilage, fat and muscle cells and are being investigated for their utility in cell-based transplantation therapy. Yet, adequate methods to track transplanted MSCs <it>in vivo </it>are limited, precluding functional studies. Quantum Dots (QDs) offer an alternative to organic dyes and fluorescent proteins to label and track cells <it>in vitro </it>and <it>in vivo</it>. These nanoparticles are resistant to chemical and metabolic degradation, demonstrating long term photostability. Here, we investigate the cytotoxic effects of <it>in vitro </it>QD labeling on MSC proliferation and differentiation and use as a cell label in a cardiomyocyte co-culture.</p> <p>Results</p> <p>A dose-response to QDs in rat bone marrow MSCs was assessed in Control (no-QDs), Low concentration (LC, 5 nmol/L) and High concentration (HC, 20 nmol/L) groups. QD yield and retention, MSC survival, proinflammatory cytokines, proliferation and DNA damage were evaluated in MSCs, 24 -120 hrs post QD labeling. In addition, functional integration of QD labeled MSCs in an <it>in vitro </it>cardiomyocyte co-culture was assessed. A dose-dependent effect was measured with increased yield in HC vs. LC labeled MSCs (93 ± 3% vs. 50% ± 15%, p < 0.05), with a larger number of QD aggregates per cell in HC vs. LC MSCs at each time point (p < 0.05). At 24 hrs >90% of QD labeled cells were viable in all groups, however, at 120 hrs increased apoptosis was measured in HC vs. Control MSCs (7.2% ± 2.7% vs. 0.5% ± 0.4%, p < 0.05). MCP-1 and IL-6 levels doubled in HC MSCs when measured 24 hrs after QD labeling. No change in MSC proliferation or DNA damage was observed in QD labeled MSCs at 24, 72 and 120 hrs post labeling. Finally, in a cardiomyocyte co-culture QD labeled MSCs were easy to locate and formed functional cell-to-cell couplings, assessed by dye diffusion.</p> <p>Conclusion</p> <p>Fluorescent QDs label MSC effectively in an <it>in vitro </it>co-culture model. QDs are easy to use, show a high yield and survival rate with minimal cytotoxic effects. Dose-dependent effects suggest limiting MSC QD exposure.</p

Lithospheric structure of an Archean craton and adjacent mobile belt revealed from 2-D and 3-D inversion of magnetotelluric data : example from southern Congo craton in northern Namibia

Author Posting. © American Geophysical Union, 2013. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Solid Earth 118 (2013): 4378–4397, doi:10.1002/jgrb.50258.Archean cratons, and the stitching Proterozoic orogenic belts on their flanks, form an integral part of the Southern Africa tectonic landscape. Of these, virtually nothing is known of the position and thickness of the southern boundary of the composite Congo craton and the Neoproterozoic Pan-African orogenic belt due to thick sedimentary cover. We present the first lithospheric-scale geophysical study of that cryptic boundary and define its geometry at depth. Our results are derived from two-dimensional (2-D) and three-dimensional (3-D) inversion of magnetotelluric data acquired along four semiparallel profiles crossing the Kalahari craton across the Damara-Ghanzi-Chobe belts (DGC) and extending into the Congo craton. Two-dimensional and three-dimensional electrical resistivity models show significant lateral variation in the crust and upper mantle across strike from the younger DGC orogen to the older adjacent cratons. We find Damara belt lithosphere to be more conductive and significantly thinner than that of the adjacent Congo craton. The Congo craton is characterized by very thick (to depths of  250 km) and resistive (i.e., cold) lithosphere. Resistive upper crustal features are interpreted as caused by igneous intrusions emplaced during Pan-African magmatism. Graphite-bearing calcite marbles and sulfides are widespread in the Damara belt and account for the high crustal conductivity in the Central Zone. The resistivity models provide new constraints on the southern extent of the greater Congo craton and suggest that the current boundary drawn on geological maps needs revision and that the craton should be extended further south.The SAMTEX consortiummembers (Dublin Institute for Advanced Studies, Woods Hole Oceanographic Institution, Council for Geoscience (South Africa), De Beers Group Services, The University of the Witwatersrand, Geological Survey of Namibia, Geological Survey of Botswana, Rio Tinto Mining and Exploration, BHP Billiton, Council for Scientific and Industrial Research (South Africa), and ABB Sweden) are thanked for their funding and logistical support during the four phases of data acquisition. This work is also supported by research grants from the National Science Foundation (EAR-0309584 and EAR-0455242 through the Continental Dynamics Program to R. L. Evans), the Department of Science and Technology, South Africa, and Science Foundation of Ireland (grant 05/RFP/ GEO001to A. G. Jones).2014-02-0

Kinematic constraints on the Rodinia to Gondwana transition

Earth's plate tectonic history during the breakup of the supercontinent Pangea is well constrained from the seafloor spreading record, but evolving plate configurations during older supercontinent cycles are much less well understood. A relative paucity of available palaeomagnetic and geological data for deep time reconstructions necessitates innovative approaches to help discriminate between competing plate configurations. More difficult is tracing the journeys of individual continents during the amalgamation and breakup of supercontinents. Typically, deep-time reconstructions are built using absolute motions defined by palaeomagnetic data, and do not consider the kinematics of relative motions between plates, even for occasions where they are thought to be ‘plate-pairs’, either rifting apart leading to the formation of conjugate passive margins separated by a new ocean basin, or brought together by collision and orogenesis. Here, we use open-source software tools (GPlates/pyGPlates) to assess quantitative plate kinematics inherent within alternative reconstructions, such as rates of relative plate motion. We analyse the Rodinia-Gondwana transition during the Neoproterozoic, investigating the proposed Australia-Laurentia configurations during Rodinia, and the motion of India colliding with Gondwana. We find that earlier rifting times provide more optimal kinematic results. The AUSWUS and AUSMEX configurations with rifting at 800 Ma are the most kinematically supported configurations for Australia and Laurentia (average rates of 57 and 64 mm/a respectively), and angular rotation of ∼1.4°/Ma, compared to a SWEAT configuration (average spreading rate ∼76 mm/a) and Missing-Link configuration (∼90 mm/a). Later rifting, at, or after, 725 Ma necessitates unreasonably high spreading rates of >130 mm/a for AUSWUS and AUSMEX and ∼150 mm/a for SWEAT and Missing-Link. Using motion paths and convergence rates, we create a kinematically reasonable (convergence below 70 mm/a) tectonic model that is built upon a front-on collision of India with Gondwana, while also incorporating sinistral strike-slip motion against Australia and East Antarctica. We use this simple tectonic model to refine a global model for the breakup of western Rodinia and the transition to eastern Gondwana. © 2017 Elsevier B.V.This manuscript is a contribution to IGCP projects 628 (Gondwana Map) and 648 (Supercontinent Cycles and Global Geodynamics). This research was supported by the Science Industry Endowment Fund (RP 04-174) Big Data Knowledge Discovery Project, Australian Research Council grant DP130101946 (RDM) and the AuScope NCRIS project. ASM is supported by a CSIRO-Data61 Postgraduate Scholarship. ASC's contribution forms TRaX Record #379 and was funded by an Australian Research Council Future Fellowship FT120100340

Kinematic constraints on the Rodinia to Gondwana transition

Earth's plate tectonic history during the breakup of the supercontinent Pangea is well constrained from the seafloor spreading record, but evolving plate configurations during older supercontinent cycles are much less well understood. A relative paucity of available palaeomagnetic and geological data for deep time reconstructions necessitates innovative approaches to help discriminate between competing plate configurations. More difficult is tracing the journeys of individual continents during the amalgamation and breakup of supercontinents. Typically, deep-time reconstructions are built using absolute motions defined by palaeomagnetic data, and do not consider the kinematics of relative motions between plates, even for occasions where they are thought to be ‘plate-pairs’, either rifting apart leading to the formation of conjugate passive margins separated by a new ocean basin, or brought together by collision and orogenesis. Here, we use open-source software tools (GPlates/pyGPlates) to assess quantitative plate kinematics inherent within alternative reconstructions, such as rates of relative plate motion. We analyse the Rodinia-Gondwana transition during the Neoproterozoic, investigating the proposed Australia-Laurentia configurations during Rodinia, and the motion of India colliding with Gondwana. We find that earlier rifting times provide more optimal kinematic results. The AUSWUS and AUSMEX configurations with rifting at 800 Ma are the most kinematically supported configurations for Australia and Laurentia (average rates of 57 and 64 mm/a respectively), and angular rotation of ∼1.4°/Ma, compared to a SWEAT configuration (average spreading rate ∼76 mm/a) and Missing-Link configuration (∼90 mm/a). Later rifting, at, or after, 725 Ma necessitates unreasonably high spreading rates of >130 mm/a for AUSWUS and AUSMEX and ∼150 mm/a for SWEAT and Missing-Link. Using motion paths and convergence rates, we create a kinematically reasonable (convergence below 70 mm/a) tectonic model that is built upon a front-on collision of India with Gondwana, while also incorporating sinistral strike-slip motion against Australia and East Antarctica. We use this simple tectonic model to refine a global model for the breakup of western Rodinia and the transition to eastern Gondwana. © 2017 Elsevier B.V.This manuscript is a contribution to IGCP projects 628 (Gondwana Map) and 648 (Supercontinent Cycles and Global Geodynamics). This research was supported by the Science Industry Endowment Fund (RP 04-174) Big Data Knowledge Discovery Project, Australian Research Council grant DP130101946 (RDM) and the AuScope NCRIS project. ASM is supported by a CSIRO-Data61 Postgraduate Scholarship. ASC's contribution forms TRaX Record #379 and was funded by an Australian Research Council Future Fellowship FT120100340

An inter-subunit protein-peptide interface that stabilizes the specific activity and oligomerization of the AAA+ chaperone Reptin

The work was supported by: the Czech Science Foundation 16-20860S (PM, LH) and 16-07321S (BV, TH), the project MEYS – NPS I – LO1413, and MH CZ - DRO (MMCI, 00209805); the BBSRC RASOR consortium (BB/C511599/1; United Kingdom); Cancer Research UK (C21383/A6950); The International Centre for Cancer Vaccine Science project carried out within the International Research Agendas programme of the Foundation for Polish Science co-financed by the European Union under the European Regional Development Fund; A*STAR, Singapore and NSCC, Singapore.Reptin is a member of the AAA+ superfamily whose members can exist in equilibrium between monomeric apo forms and ligand bound hexamers. Inter-subunit protein-protein interfaces that stabilize Reptin in its oligomeric state are not well-defined. A self-peptide binding assay identified a protein-peptide interface mapping to an inter-subunit “rim” of the hexamer bridged by Tyrosine-340. A Y340A mutation reduced ADP-dependent oligomer formation using a gel filtration assay, suggesting that Y340 forms a dominant oligomer stabilizing side chain. The monomeric ReptinY340A mutant protein exhibited increased activity to its partner protein AGR2 in an ELISA assay, further suggesting that hexamer formation can preclude certain protein interactions. Hydrogen-deuterium exchange mass spectrometry (HDX-MS) demonstrated that the Y340A mutation attenuated deuterium suppression of Reptin in this motif in the presence of ligand. By contrast, the tyrosine motif of Reptin interacts with a shallower pocket in the hetero-oligomeric structure containing Pontin and HDX-MS revealed no obvious role of the Y340 side chain in stabilizing the Reptin-Pontin oligomer. Molecular dynamic simulations (MDS) rationalized how the Y340A mutation impacts upon a normally stabilizing inter-subunit amino acid contact. MDS also revealed how the D299N mutation can, by contrast, remove oligomer de-stabilizing contacts. These data suggest that the Reptin interactome can be regulated by a ligand dependent equilibrium between monomeric and hexameric forms through a hydrophobic inter-subunit protein-protein interaction motif bridged by Tyrosine-340. Significance Discovering dynamic protein-protein interactions is a fundamental aim of research in the life sciences. An emerging view of protein-protein interactions in higher eukaryotes is that they are driven by small linear polypeptide sequences; the linear motif. We report on the use of linear-peptide motif screens to discover a relatively high affinity peptide-protein interaction for the AAA+ and pro-oncogenic protein Reptin. This peptide interaction site was shown to form a ‘hot-spot’ protein-protein interaction site, and validated to be important for ligand-induced oligomerization of the Reptin protein. These biochemical data provide a foundation to understand how single point mutations in Reptin can impact on its oligomerization and protein-protein interaction landscape.PostprintPeer reviewe