Immunity against Helminths: Interactions with the Host and the Intercurrent Infections

Helminth parasites are of considerable medical and economic importance. Studies of the immune response against helminths are of great interest in understanding interactions between the host immune system and parasites. Effector immune mechanisms against tissue-dwelling helminths and helminths localized in the lumen of organs, and their regulation, are reviewed. Helminth infections are characterized by an association of Th2-like and Treg responses. Worms are able to persist in the host and are mainly responsible for chronic infection despite a strong immune response developed by the parasitized host. Two types of protection against the parasite, namely, premune and partial immunities, have been described. Immune responses against helminths can also participate in pathogenesis. Th2/Treg-like immunomodulation allows the survival of both host and parasite by controlling immunopathologic disorders and parasite persistence. Consequences of the modified Th2-like responses on co-infection, vaccination, and inflammatory diseases are discussed

A dupla face do advogado mediador e especialista em direito: exemplo da negociação de um contrato transcultural de cessão de direitos autorais

A mediação não é mero modo de resolução dos conflitos, pois abre um vasto leque de atividades que favorecem e permitem a emergência de direito negociado coletivamente, inclusive em contexto transcultural. Baseando-se em um caso concreto da cessão de direitos autorais das índias brasileiras kadiwéus para a realização de obra na Alemanha, por intermédio de um escritório de arquitetura de São Paulo, neste breve comentário procura-se demonstrar que a mediação permitiu transcender as visões de mundo a fim de que cada parte envolvida pudesse ter suas questões mais importantes representadas em um acordo comum. Trata-se da maneira como autonomias e diferenças são respeitadas e é facilitado o diálogo para a procura da solução mais adequada

Water quality and water-use conflicts in Lake Taabo (Ivory Coast)

The Lake Taabo (Ivory Coast, Africa) results of the construction of the Taabo dam on the Ban- dama River. The changes in the water level of the 69-km² lake depend on 1) the rainfall linked to alternating dry/wet seasons; 2) the extraction of water for human uses; 3) the discharge of water from the upstream dam and the volumes tur- bined by the Kossou dam; 4) the various an- thropic effects (discharge of untreated waste water from towns and industries, and leaching from agricultural land). The average concentra- tions of nutrients (NH4-N: 1.1 mg/L, NO3-N: 1.62 mg/L, PO4-P: 10 mg/L, SiO2: 15 mg/L) and chlo- rophyll a (from 4.8 to 16.5 μg/L, average 11.4 μg/L) indicates some degree of eutrophication. The cumulated effects that threaten the ecosys- tem (degradation of water quality and eutrophi- cation) are such that they are likely to interfere with various water uses. In a context of growing health and environmental concerns in Africa, this study demonstrates conflicts between dif- ferent uses of this water resource and the urgent need for an appropriate policy including specific monitoring of lake water quality, wastewater control, and a programme to reduce agricultural fertilizers

The invasion process of bovine erythrocyte by Babesia divergens: knowledge from an in vitro assay

Babesia divergens is a tick-transmitted apicomplexan parasite for which asexual multiplication in its vertebrate hosts is restricted to erythrocytes. Current knowledge of invasion of these target cells is limited. An efficient in vitro invasion assay was set up to gain access to this information. Parasites prepared from infected RBC, lysed by electroporation, and mixed with bovine RBC in a selected synthetic medium (RPMI 1640 supplemented with calcium) were able to establish subsequent cultures with parasitemia ranging from 6 to 14%. Free parasites remaining in the invasion medium could be eliminated by Percoll gradient and culture could be pursued with the freshly invaded erythrocytes. In this way, the invasion time window could be shortened to obtain a synchronised start of the culture or to study the kinetics of invasion. With this assay we demonstrate that 1) erythrocyte invasion by B. divergens is a rapid process since 70% of the invasion-competent parasites invaded the RBC in less than 45 s; 2) all invasion-competent parasites achieved invasion within 10 min of contact; 3) one erythrocyte could be invaded concomitantly by two merozoites; 4) despite a synchronous start, the parasite population evolved heterogeneously resulting in a progressive loss of synchronisation. Western blot analysis of proteins collected from invasion medium were performed with sera from animals experimentally infected with B. divergens and highlighted several proteins. The dose-dependent, inhibitory effects of these sera on B. divergens invasion suggest that these proteins might be involved in the invasion process. Further investigations are required for their characterisation