14 research outputs found
Efecto del gen BMP15 sobre la distribución del tipo de parto en ovejas Rasa Aragonesa
Publishe
Development of a SNP parentage assignment panel in some North-Eastern Spanish meat sheep breeds
Aim of study: To validate two existing single nucleotide polymorphism (SNP) panels for parentage assignment in sheep, and develop a cost effective genotyping system to use in some North-Eastern Spanish meat sheep populations for accurate pedigree assignment.Area of study: SpainMaterial and methods: Nine sheep breeds were sampled: Rasa Aragonesa (n=38), Navarra (n=39), Ansotana (n=41), Xisqueta (n=41), Churra Tensina (n=38), Maellana (39), Roya Bilbilitana (n=24), Ojinegra (n=36) and Cartera (n=39), and these animals were genotyped with the Illumina OvineSNP50 BeadChip array. Genotypes were extracted from the sets of 249 SNPs and 163 SNPs for parentage assignment designed in France and North America, respectively. Validation of a selected cost-effective genotyping panel of 158 SNPs from the French panel were performed by Kompetitive allele specific PCR (KASP). Additionally, some functional SNPs (n=15) were also genotyped.Main results: The set of 249 SNPs for parentage assignment showed better diversity, probability of identity, and exclusion probabilities than the set of 163 SNPs. The average minor allele frequency for the set of 249, 163 and 158 SNPs were 0.41 + 0.01, 0.39 + 0.01 and 0.42 + 0.01, respectively. The parentage assignment rate was highly dependent to the percentage of putative sires genotyped.Research highlights: The described method is a cost-effective genotyping system combining the genotyping of SNPs for the parentage assignment with some functional SNPs, which was successfully used in some Spanish meat sheep breeds
Relationship between the effects of the BMP15 gene and the polygenic effects on prolificacy in the rasa Aragonesa sheep breed
Los efectos del gen BMP15, localizado en el cromosoma X, sobre la media y la variabilidad de la prolificidad fueron estimados en la población Rasa Aragonesa, donde se analizaron 918.956 partos de ovejas de distintos genotipos (heterocigotas para el alelo FecXR o ROA®; y no portadoras). En primer lugar, se utilizaron modelos umbral que incluían o no el efecto del gen para estimar el efecto del genotipo BMP15 y su contribución a la variabilidad genética de la prolificidad en esta población. También se utilizaron otros dos modelos para estimar las interacciones entre el genotipo y los poligenes, así como el efecto del genotipo sobre la variabilidad de la prolificidad. Todos los análisis se hicieron con el programa ASReml. El efecto del genotipo sobre la media fue de 0,32 corderos adicionales por parto cuando el alelo ROA® se encontraba presente. Debido a que durante muchos años la selección por prolificidad en esta población se ha llevado a cabo desconociendo la presencia de esta mutación, los animales selectos portadores tienen un valor genético poligénico residual más bajo que los no portadores. No se encontró interacción significativa entre el genotipo y los efectos poligénicos sobre el valor genético. Aunque la interacción entre el genotipo y el conjunto de umbrales fue significativa, la diferencia entre ambos genotipos en la varianza de la prolificidad sobre la escala observada a una prolificidad media dada es escasa y muy poco relevanteThe effects of the BMP15 gene, located on the X chromosome, on mean prolificacy and its variability, were estimated in the Rasa Aragonesa sheep population through the analysis of 918,956 lambing records from ewes of different genotypes (FecXR or ROA® heterozygous ewes; and non-carrier ewes). Threshold models including or not the gene effect were first run to determine the effect of the BMP15 genotype and its importance in the total genetic variability of prolificacy. Two other models were also run to estimate the interaction between the BMP15 genotype and the polygenic background, as well as the effect of the genotype on the variability prolificacy. All the models were run using the ASReml software. The effect of the presence of the ROA® allele of the BMP15 gene on the mean prolificacy was 0.32 extra lambs per lambing. Due to the selection on prolificacy performed during many years in this population ignoring the presence of this major gene, animals carrying the mutation were found to have lower remaining polygenic estimated breeding values than non-carrier animals, and there was no interaction between the BMP15 genotype and the polygenic background. Although the interaction between the genotype and the set of thresholds was significant, the resulting between-genotypes difference of variance of prolificacy on the observed scale, at a similar mean litter size, was very low and not relevantPublishe
Anti-Müllerian hormone plasma concentration in prepubertal ewe lambs as a predictor of their fertility at a young age
Plasma was taken from 76 Rasa Aragonesa ewe lambs at 3.6 months of age for AMH determination. Simultaneously, 600 IU equine chorionic gonadotropin (eCG) was administered and the number of ovulations recorded 6 days later. AMH was detected in 93% of the lambs, and the concentrations were about 3-4-fold higher in ovulating than in non-ovulating lambs (P = 97 pg/mL than in those with lower AMH concentrations (50% vs. 15%; P < 0.001)
Desarrollo y estado actual del Banco de Recursos Genéticos de las razas autóctonas en peligro de extinción de ganado ovino en Aragón
La crioconservación de material reproductivo y la formación de Bancos de Recursos Genéticos Animales es fundamental en el desarrollo de los Programas de Mejora, Conservación y Fomento ganadero. Las Administraciones Públicas junto con las Asociaciones de Ganaderos son los principales actores en la creación de los mismos. El objeto de la comunicación pone de relieve las actuaciones que se están llevando a cabo para la creación del Banco de Recursos Genéticos (BRG) de razas autóctonas aragonesas de ganado ovino en peligro de extinciónPublishe
Niveles del plaguicida disruptor endocrino “lindano” en ganado ovino de una zona posiblemente contaminada
El lindano es un plaguicida organoclorado que ha sido
muy utilizado en agricultura y también en salud pública
para el control de vectores, presentando la capacidad de
ser un disruptor endocrino. Actualmente su empleo está
prohibido en los países occidentales, aunque el riesgo
de exposición no ha desaparecido todavía debido a su
carácter persistente en el medio ambiente, así como por
su capacidad de acumulación en la grasa de los tejidos.
La oveja, a pesar de no ser un animal de vida libre, puede
ser utilizada en la biomonitorización ambiental de la
presencia de contaminantes orgánicos persistentes en
los ecosistemas en los que se desarrolla
Innovations in the selection program of the UPRA-Grupo Pastores in Rasa aragonesa sheep breed
The Cooperative Oviaragon-Grupo Pastores carries out since 1994 a selection program for prolificacy in
Rasa Aragonesa sheep, with 216.232 ewes at present. Sires of higher estimated breeding values are
produced within the scheme by MOET, increasing the performance of this technology through the
measurement of plasmatic Anti-Müllerian Hormone (AMH) in ewe embryo donors, an endocrine marker
of the ovarian activity. Within this program a natural genetic prolific variant was found in 2007 (FecXR
allele, BMP15 gene). Its effect on prolificacy leads to an increase of 0.35 lambs/lambing ewe when
compared with non-carrier ewes, with an additive effect over the standard hormonal treatments used in
farms. Recently, new variants in MTNR1A gene associated to reproductive seasonality have been
detected in Rasa aragonesa breed. Non-linked SNPs in promoter and exon 2 regions have been detected
decreasing the length of non-cycling period (considering anoestrus those periods with three or more
consecutive weekly-sampled progesterone concentrations lower than 0.5 ng/ml) as much as 30 and 53
days, respectively, from January to August. In the same way, the allele located in exon 2 was also
associated to an increase of 15 % of oestrus cycling months (based on oestrus records). Due to their
productive interest, a controlled program for the outreach of FecXR allele and MTNR1A alleles has been
developed. Finally, a polygenic selection program for maternal capacity is being carried out. The
selection program goes on with a combined polygenic selection for prolificacy, maternal capacity and
dissemination of FecXR and MTNR1A alleles
Monitorización de la actividad sexual en corderas rasa aragonesa mediante la detección automática de celos
Financiado a través del proyecto Interreg VA POCTEFA “PIRINNOVI-EFA103/15”.Publishe
Innovations in the selection program of the UPRA-Grupo Pastores in Rasa aragonesa sheep breed
Raza Rasa aragonesaPublishe
Genome-wide association study (GWAS) identifies the FecXGr allele in BMP15 segregating in Rasa Aragonesa sheep breed
Raza Rasa aragonesaPublishe