α-glucosidase inhibitors isolated from Mimosa pudica L.

The aim of the study was to isolate digestive enzymes inhibitors from Mimosa pudica through a bioassay-guided fractionation approach. Repeated silica gel and sephadex LH 20 column chromatographies of bioactive fractions afforded stigmasterol, quercetin and avicularin as digestive enzymes inhibitors whose IC50 values as compared to acarbose (351.02 ± 1.46 μg mL−1) were found to be as 91.08 ± 1.54, 75.16 ± 0.92 and 481.7 ± 0.703 μg mL−1, respectively. In conclusion, M. pudica could be a good and safe source of digestive enzymes inhibitors for the management of diabetes in future

Enrichment, in vitro, and quantification study of antidiabetic compounds from neglected weed Mimosa pudica using supercritical CO2 and CO2-Soxhlet

Supercritical fluid extraction (SFE) using carbon dioxide (CO2) and liquid CO2 using Soxhlet (CO2-Soxhlet) extraction were employed to extract three (3) antidiabetic compounds viz. stigmasterol, quercetin, and avicularin from Mimosa pudica. Various extraction parameters were studied. Extracts were analyzed pharmacologically, qualitatively and quantitatively to ascertain enrichment levels. All three antidiabetic compounds were effectively enriched under optimized conditions of temperature 60°C, pressure 40 MPa, co-solvent ratio 30%, and CO2 flow rate of 5 ml min−1. SFE was found to be the better method for enrichment of the antidiabetic compounds than the CO2-Soxhlet method. Extraction conditions were seen to affect the enrichment of desired compounds

Supercritical fluid extraction of phenolic compounds and antioxidants from grape (Vitis labrusca B.) seeds. Plant Foods for Human Nutrition

Abstract Supercritical fluid extraction (SFE) technique was applied and optimized for temperature, CO 2 pressure and ethanol (modifier) concentration using orthogonal array design and response surface methodology for the extract yield, total phenols and antioxidants from grape (Vitis labrusca B.) seeds. Effects of extraction temperature and pressure were found to be significant for all these response variables in SFE process. Optimum SFE conditions (444 6°C temperature and 153~161 bar CO 2 pressure) along with ethanol (<7 %) as modifier, for the maximum predicted values of extract yield (12.09 %), total phenols (2.41 mg GAE/ml) and antioxidants (7.08 mg AAE/ml), were used to obtain extracts from grape seeds. The predicted values matched well with the experimental values (12.32 % extract yield, 2.45 mg GAE/ml total phenols and 7.08 mg AAE/ml antioxidants) obtained at optimum SFE conditions. The antiradical assay showed that SFE extracts of grape seeds can scavenge more than 85 % of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The grape seeds extracts were also analyzed for hydroxybenzoic acids which included gallic acid (1.21~3.84 μg/ml), protocatechuic acid (3.57~11.78 μg/ml) and p-hydroxybenzoic acid (206.72~688.18 μg/ml)

Non-Bovine Milk: Sources and Future Prospects

Milk is the first food that mammals are exposed to [...

The effect of harvest periods on the chemical compositions of essential oils of sage (Salvia aucheri L.) leave.

International audienceThe essential oils of sage leaves (Salvia aucheri Bentham var. canescens Boiss. & Heldr.), growing wild in South Anatolia, were extracted by hydrodistillation and analysed by GC and GC-MS. The percentage yields of the essential oils from sage leaves harvested at different years were 1.0%, 1.3%, 1.3%, 1.0%, 1.4%, 1.5% and 1.2%, respectively. In this study, 1,8-cineole, camphre, camphene α-pinene and β-pinene were identified as the major components of sage leaves collected at different periods. The main constituents of sage oil collected over the years were 1,8-cineole (35.01-48.06%), camphre (13.58-23.92%), camphene (6.77-8.82%), α-pinene (5.79-8.54%) and β-pinene (4.32-6.28%)

Consumers’ Perceptions and Sensory Properties of Beef Patty Analogues

The present study was carried out to gain consumer insights on the use of tempeh (a fermented soy bean product) to improve the healthiness of beef patties and to determine the acceptable level of tempeh (10%, 20%, or 30%) in the patty. The study consisted of conducting two focus groups (n = 15), a pilot sensory evaluation, and a full consumer sensory study. The focus groups were asked about their consumption of beef patties, attitudes towards processed meat, attitudes towards negative aspects of red meat consumption, and attitudes towards tempeh consumption, as well as sensory perceptions of the cooked patties and their visual acceptance of raw patties. Focus group discussions suggested that there was a market for the product if consumers were informed of tempeh health benefits. Participants seemed more willing to choose how to balance their diet with an antioxidant source than buy a beef patty with added antioxidants. The focus group participants rated the visual attributes of raw patties from all treatments and it was found that the 20% tempeh and 30% tempeh patties were ranked lower (p < 0.05) than the others. Overall, the sensory experiments showed that the inclusion of 10% tempeh was the most acceptable level of addition. There were no significant (p > 0.05) differences between the control and 10% tempeh patties for overall acceptability or acceptance of flavor. However, 10% tempeh patties were found to be more tender and juicier than the control (p < 0.05). A proper knowledge and awareness of consumers about the benefits of tempeh could allow the development of beef containing tempeh products

Biogas Production through the Anaerobic Digestion of Date Palm Tree Wastes - Process Optimization

A process for the production of biogas through the anaerobic digestion (AD) of date palm tree waste (DPTW) was developed. The effects of different substrate pretreatments and operating conditions on the yield of biogas and on the chemical composition of DPTW before and after AD were studied. The best results were obtained using alkali pretreatment, with a particle size of 2 to 5 mm, a C:N ratio of 30:1, a digestion temperature of 40 °C, an initial pH of 7.0, and a volatile solids concentration of approximately 10%. The production of flammable biogas containing up to 50% methane started after about one week of operation and continued for approximately 11 weeks. The highest average biogas yield obtained was 342.2 L gas/kg of volatile solids fed to the digester. The highest maximum and average volumetric biogas production rates obtained were 674.5 and 404.4 L/m3 of digester volume per day, respectively. After digestion, there was up to a 58% reduction in the organic matter content of the substrate. Reductions in the contents of cellulose, hemicellulose, and soluble organic compounds were 68.7, 73.4, and 71.9%, respectively, while the ash and lignin contents remained mostly constant. The remaining sludge contained nutrient minerals and some organic matter which qualifies it as a potential soil fertilizer for crop production