The Role of Tourism Media in the Promotion of Domestic Tourism in Jordan (Governorate of Irbid as A model)

The role of Tourism Media in the promotion of domestic tourism in Jordan is limited, which affected the process of attracting tourists locally and internationally.Studies that dealt with the relationship between tourism and tourism media are limited, therefore, the importance of this study stems from the fact that it`s based on data and information collected from a sample of visitors to the archaeological and tourist sites in Irbid Governorate to identify the role of tourism media in the promotion of domestic tourism, particularly promoting the Jordanian tourism product in Irbid. This study also makes recommendations to the authorities concerned with promoting tourism in Jordan, to help developing and improving tourism media in the country. Keywords: Tourism media,archaeological,domestic tourism,tourism product,promption

Investigating the tumor-immune microenvironment through extracellular vesicles from frozen patient biopsies and 3D cultures

Melanomas are highly immunogenic tumors that have been shown to activate the immune response. Nonetheless, a significant portion of melanoma cases are either unresponsive to immunotherapy or relapsed due to acquired resistance. During melanomagenesis, melanoma and immune cells undergo immunomodulatory mechanisms that aid in immune resistance and evasion. The crosstalk within melanoma microenvironment is facilitated through the secretion of soluble factors, growth factors, cytokines, and chemokines. In addition, the release and uptake of secretory vesicles known as extracellular vesicles (EVs) play a key role in shaping the tumor microenvironment (TME). Melanoma-derived EVs have been implicated in immune suppression and escape, promoting tumor progression. In the context of cancer patients, EVs are usually isolated from biofluids such as serum, urine, and saliva. Nonetheless, this approach neglects the fact that biofluid-derived EVs reflect not only the tumor, but also include contributions from different organs and cell types. For that, isolating EVs from tissue samples allows for studying different cell populations resident at the tumor site, such as tumor-infiltrating lymphocytes and their secreted EVs, which play a central anti-tumor role. Herein, we outline the first instance of a method for EV isolation from frozen tissue samples at high purity and sensitivity that can be easily reproduced without the need for complicated isolation methods. Our method of processing the tissue not only circumvents the need for hard-to-acquire freshly isolated tissue samples, but also preserves EV surface proteins which allows for multiplex surface markers profiling. Tissue-derived EVs provide insight into the physiological role of EVs enrichment at tumor sites, which can be overlooked when studying circulating EVs coming from different sources. Tissue-derived EVs could be further characterized in terms of their genomics and proteomics to identify possible mechanisms for regulating the TME. Additionally, identified markers could be correlated to overall patient survival and disease progression for prognostic purposes

Topics of Imaging in Al-buhturi Poetry

This research dealt with the topics of Imaging in the poetry of al-Buhturi, three topics were chosen: women, nature and animals. There is a relationship between other arts, such as the plastic arts of drawing and sculpture, and the poetry written by Al-Buhturi, where poetic subjects appear in figurative mechanisms that seem like artistic paintings that derive their material from the letter and the word. This study seeks to reveal the manifestations of photography in the three poetic themes according to the descriptive analytical approach. The results of the study showed Al-Buhturi\u27s ability to form images and produce them as artistic paintings that excite the reader and entice him to interact with them

The Image of Nature and Human in Elia Abu Madi Poetry: The Woman and Homeland as a Model

The study deals with the image of nature and the human relationship with it in light of the knowledge of the relationship with women and the homeland. The study seeks to analyze the relationship between nature and man, in which the poet\u27s vision was manifested. The study highlighted, through its topics related to women and the homeland, the depth of the connection between the poet and nature. The study confirms the importance of nature in Abu Madi\u27s poetry as an idea that expresses the intellectual and psychological dimensions with which his poems were mixed and expressed. The current study shows the image of nature and its connection with man in his perceptions and idea

Modelling liver cancer microenvironment: novel 3D culture system as a potential anti-cancer drug screening tool

The tumor microenvironment and its contribution to tumorigenesis has been a focal highlight in recent years. A two-way communication between the tumor and the surrounding microenvironment sustains and contributes to the growth and metastasis of tumors. Progression and metastasis of hepatocellular carcinoma have been reported to be exceedingly influenced by diverse microenvironmental cues. In this study, we present a 3D-culture model of liver cancer to better mimic in vivo tumor settings. By creating novel 3D co-culture model that combines free-floating and scaffold based 3D-culture techniques of liver cancer cells and fibroblasts, we aimed to establish a simple albeit reproducible ex vivo cancer microenvironment model that captures tumor-stroma interactions. The model presented herein exhibited unique gene expression and protein expression profiles when compared to 2D and 3D mono-cultures of liver cancer cells. Our results showed that in vivo like conditions cannot be mimicked by simply growing cancer cells as spheroids, but by co-culturing them with 3D fibroblast with which they were able to cross-talk. This was evident by the upregulation of several pathways involved in HCC, and the increase in secreted factors by co-cultured cancer cells, many of which are also involved in tumor-stroma interactions. Compared to the conventional 2D culture, the proposed model exhibits an increase in the expression of genes associated with development, progression, and poor prognosis of HCC. Our results correlated with an aggressive outcome that better mirrors in vivo HCC, and therefore, a more reliable platform for molecular understanding of HCC and possibly better anti-cancer drug screening

Modelling liver cancer microenvironment using a novel 3D culture system

The tumor microenvironment and its contribution to tumorigenesis has been a focal highlight in recent years. A two-way communication between the tumor and the surrounding microenvironment sustains and contributes to the growth and metastasis of tumors. Progression and metastasis of hepatocellular carcinoma (HCC) have been reported to be exceedingly influenced by diverse microenvironmental cues. In this study, we present a 3D-culture model of liver cancer to better mimic in vivo tumor settings. By creating novel 3D co-culture model that combines free-floating and scaffold-based 3D-culture techniques of liver cancer cells and fibroblasts, we aimed to establish a simple albeit reproducible ex vivo cancer microenvironment model that captures tumor-stroma interactions. The model presented herein exhibited unique gene expression and protein expression profiles when compared to 2D and 3D mono-cultures of liver cancer cells. Our results showed that in vivo like conditions cannot be mimicked by simply growing cancer cells as spheroids, but by co-culturing them with 3D fibroblast with which they were able to crosstalk. This was evident by the upregulation of several pathways involved in HCC, and the increase in secreted factors by co-cultured cancer cells, many of which are also involved in tumor-stroma interactions. Compared to the conventional 2D culture, the proposed model exhibits an increase in the expression of genes associated with development, progression, and poor prognosis of HCC. Our results correlated with an aggressive outcome that better mirrors in vivo HCC, and therefore, a more reliable platform for molecular understanding of HCC

Therapeutic potential of active components of saffron in post-surgical adhesion band formation

Background Abdominal adhesions are common and often develop after abdominal surgery. There are currently no useful targeted pharmacotherapies for adhesive disease. Saffron and its active constituents, Crocin and Crocetin, are wildly used in traditional medicine for alleviating the severity of inflammatory or malignant disease. Purpose The aim of this study was to investigate the therapeutic potential of the pharmacological active component of saffron in attenuating the formation of post-operative adhesion bands using different administration methods in a murine model. Material method saffron extract (100 mg/kg), Crocin (100 mg/kg), and Crocetin (100 mg/kg) were administered intraperitoneally and by gavage in various groups of male Wistar rat post-surgery. Also three groups were first treated intra-peritoneally by saffron extract, Crocin, and Crocetin (100 mg/kg) for 10 days and then had surgery. At the end of the experiments, animals sacrificed for biological assessment. Result A hydro-alcoholic extract of saffron and crocin but not crocetin potently reduced the adhesion band frequency in treatment and pre-treatment groups in the mice given intra-peritoneal (i.p) injections. Following the saffron or crocin administration, histological evaluation and quantitative analysis represented less inflammatory cell infiltration and less collagen composition, compared to control group. Moreover, the oxidative stress was significantly reduced in treatment groups. Conclusion These findings suggest that a hydro-alcoholic extract of saffron or its active compound, crocin, is a potentially novel therapeutic strategy for the prevention of adhesions formation and might be used as beneficial anti-inflammatory or anti-fibrosis agents in clinical trials. Taxonomy Abdominal surgeries/post-surgical adhesions

Modelling the Micro environment of Liver Cancer Using 3D Culture System

Tumor micro environment has been the focus of many studies that highlighted its essential contribution to tumorigenesis. A two-way communication between the tumor and the surrounding microenvironment sustains and contributes to the growth and metastasis of the tumor by several means, such as extracellular matrix remodeling, fibroblasts activation, epithelial-mesenchymal transition. Hepatocellular carcinoma progression and metastasis have been reported to be highly influenced by diverse microenvironmental elements. The main objective of this study is to create an in vitro model of liver cancer microenvironment that better recapitulate in vivo settings. The proposed model is based on three-dimensional co-culture system of liver cancer cells and non-malignant fibroblasts. The model presented herein exhibited a transcriptome profile associated with an aggressive phenotype that better mimics in vivo hepatocellular carcinoma, and therefore, a more reliable platform for anti-cancer drug screenin

Extracellular Vesicles Orchestrate Immune and Tumor Interaction Networks

Extracellular vesicles (EVs) are emerging as potent and intricate intercellular communication networks. From their first discovery almost forty years ago, several studies have bolstered our understanding of these nano-vesicular structures. EV subpopulations are now characterized by differences in size, surface markers, cargo, and biological effects. Studies have highlighted the importance of EVs in biology and intercellular communication, particularly during immune and tumor interactions. These responses can be equally mediated at the proteomic and epigenomic levels through surface markers or nucleic acid cargo signaling, respectively. Following the exponential growth of EV studies in recent years, we herein synthesize new aspects of the emerging immune-tumor EV-based intercellular communications. We also discuss the potential role of EVs in fundamental immunological processes under physiological conditions, viral infections, and tumorigenic conditions. Finally, we provide insights on the future prospects of immune-tumor EVs and suggest potential avenues for the use of EVs in diagnostics and therapeutics