Impact of Pre-Anthesis Water Deficit on Yield and Yield Components in Barley (\u3ci\u3eHordeum vulgare\u3c/i\u3e L.) Plants Grown under Controlled Conditions

Drought at pre-anthesis stages can influence barley growth and results in yield losses. Therefore, it is important to understand how drought at pre-anthesis can affect different traits associated with yield reduction in barley. The objective of this study was to understand the relevance of the genetic background of major flowering time genes in barley plants subjected to pre-anthesis drought and its impact on yield and yield components. A glasshouse experiment using a Randomized Complete Block Design was conducted to investigate the effect of drought and its timing on yield and yield components on eleven barley genotypes, which were selected to represent genetic diversity of major flowering time genes (PPDH1, PPDH2, HvVrn1, HvVrn2 and HvVrn3). Barley plants were exposed to three water regimes, non-stressed and stressed, which was applied at two pre-anthesis growth stages, tillering (SS) and stem elongation (SE). Results identified differences among genotypes in all measured traits. Grain yield, grain number and thousand kernel weight were reduced in all genotypes due to drought, irrespective of the growth stage. Early flowering genotypes had better performance as reflected in higher yield compared with late flowering genotypes. Results verified the fundamental importance of early flowering to improve productivity in response to pre-anthesis drought. The results of this study can help in selecting barley lines for future breeding purposes with improved resilience to drought conditions in Mediterranean environments

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

The impact of strategic human resource management on organizational resilience: an empirical study on hospitals

The aim of this study was to explore the impact of strategic human resource management practices (strategic value of human resource practices, human resource analytics and high-performance work practices) on organizational resilience (cognitive, behavioral and contextual dimensions) in private hospitals. The required data for the purpose of this study were collected by a questionnaire developed on the basis of related works. The questionnaire was developed based on exploratory and confirmatory factor analyses. It consisted of 30 items divided into two domains, the first one to measure strategic resource management practices (15 items), and the second one to measure organizational resilience (15 items). The study population was consisted of employees working at private hospitals. The questionnaires were distributed to a random sample of 500 administrative staff working in private hospitals. A total of 449 valid responses were retrieved with a response rate of 89.9%, which is high percent because the researcher visited the hospitals and distributed the questionnaires personally. Using IBM SPSS 24.0 and AMOS 22, the results confirmed that strategic human resource management practices have a positive impact on organizational resilience. In terms of the separate effects of strategic HRM practices, the results showed that strategic value of human resource practices was the most influential variable on organizational resilience, followed by human resource analytics, then high performance work practices The study concluded that the hospital’s ability to be resilient requires an advance planning when formulating the human resources strategy that is supposed to be integrated into the hospital’s strategy

Molecular and immunological diagnosis of cystic echinococcosis from different hosts in the Gaza Strip, Palestine

The aim of the present study was to determine the molecular characteristics of Echinococcus granulosus sensu lato from faecal samples of dogs and the hydatid cyst from liver of bovine, camel, cattle, and sheep and the immunodiagnosis of Hydatid cyst in human sera samples from the Gaza Strip, in Palestine. A total of 38 faecal samples were collected near farms and local markets where stray dogs were grouping there. Post-slaughtering, the livers of the bovine, camel, cattle, and sheep were examined for cysts. Where 30 suspected hydatid cysts were collected, the number and size of cysts in each liver were recorded. For serology, 23 sera specimens were collected from farmers within the Gaza Strip. According to hospital records in Gaza, 12 patients had attended three different hospitals and had tested positive for hydatid cyst/cystic echinococcosis by CT scans and histopathology. ELISA results from the 23 human patients showed that 3/23 (13%) tested positive for hydatid disease. Of the 30 bovine and cattle harboring cysts, 14/30 (46.6%) were positive for hydatid cyst/cystic echinococcosis of which 23 (76.7%) were males and 7 (23.3%) were females. The present study shows that 6/38 (15.7%) were positive for E. granulosus sensu lato from faecal analysis in dogs. Subsequent sequencing of both dogs and cattle confirmed infection by the G1 strain. The cattle/dog strain (G1) of E.granulosus sensu lato suggests that this infection is cosmopolitan in its distribution. It is concluded that the detection of hydatid cyst/cystic echinococcosis in the examined hosts either human or animals should be considered among physicians and a large sample size is recommended in future research

Esophageal foreign bodies in children: Emphasis on complicated cases

Background: Esophageal foreign bodies (EFBs) are a relatively common clinical problem in pediatric patients. The majority of EFBs pass harmlessly through the gastrointestinal tract; however, some EFBs can cause significant morbidities. This study was conducted to review our experience in managing esophageal foreign bodies in pediatric patients, with an emphasis on the management and outcomes of complicated cases. Methods: Between March 1995 and March 2013, the records of all children up to the age of 12 years who were admitted to King Khalid University Hospital, Riyadh, Saudi Arabia, with a final diagnosis of EFBs were reviewed. The medical records were analyzed with respect to demographic data, presenting symptoms, workup investigation, management, complications, and outcomes. Results: Seventy patients were identified (38 boys and 32 girls). The ages ranged from 5 days to 12 years (mean: 4.4 years). Fifty-three (75.7%) patients presented within 24 hours. Thirteen (18.6%) patients had underlying predisposing factors. The most common EFB, found in 30 (42.8%) patients, was a coin. Witnessed ingestion of a FB was documented in 52 (74.2%) patients. The most common symptoms were drooling of saliva in 42 (60%) patients, followed by vomiting in 36 (51.4%) patients. Four (5.7%) patients presented with complications secondary to FB impaction, including hypopharyngeal wall perforation, acquired esophageobronchial fistula, localized esophageal perforation with inflammation, and perforation with stricture formation. The follow-up period ranged from 2 to 12 months, and all patients had complete recovery without any sequelae. Conclusion: EFBs are a relatively common problem in pediatric patients, and underlying predisposing factors to EFB impaction are not uncommon. Long-retained EFBs are associated with a higher incidence of complications. Rigid esophagoscopy was successful in extracting most of the EFBs and was shown to be a safe and effective procedure

Age-Related Changes in Blood Lymphocyte Subsets of Saudi Arabian Healthy Children

The age-related changes in absolute and percentage values of lymphocyte subsets in the peripheral blood of healthy children of different ages (1 month to 13 years) were studied by flow cytometry. The absolute and percentage values for most lymphocyte subpopulations differed substantially with age. Comparisons among age groups from infants through adults revealed progressive declines in the absolute numbers of leukocytes, total lymphocytes, and T, B, and natural killer (NK) cells. The percentages of T cells increased with age. Within the T-lymphocyte population, the CD8(+) subset increased but the CD4(+) subset decreased, resulting in a declining CD4(+)/CD8(+) ratio. The percentage of B cells declined, but that of NK cells remained unchanged. The percentage of HLA-DR(+) T cells increased over time, but their number changed inconsistently. Our findings confirm and extend earlier reports on age-related changes in lymphocyte subpopulations. These data should be useful in the interpretation of disease-related changes, as well as therapy-dependent alterations, in lymphocyte subsets in children of different age groups