13,762 research outputs found
ENHANCED METHOD FOR AMPLIFYING ANTIMICROBIAL ACTIVITY OF LACTOPEROXIDASE SYSTEM IN MILK AND DERIVED PRODUCTS BY CARROT EXTRACT AND BETA CAROTENE
OSCN– has been known and well documented as natural antimicrobial
agent generated from the lactoperoxidase system (LPOS) but the antimicrobial
activity exerted from this system is too low for certain food such as milk and
derived products (up to 1.2 log CFU/ml) resulting in the restriction on industrial
development of LPOS. Our previous study concluded that involvement of carrot
extract and beta carotene in LPOS significantly boosted the antimicrobial activity
(up to 6 log CFU/ml) against S. enteritidis. This finding should be continued to
the application on food. Since we found that LPOS generates low antimicrobial
activity on milk and derived product, our research will be conducted on the
application of LPOS plus carrot extract and beta carotene on milk and derived
product. Because the high antimicrobial activity is needed for industrial
purposes, this research may open the way for industrial development of natural
antimicrobial agent from LPOS.
This research will be conducted in three steps of experiment: (1) ensuring
the incredible antimicrobial activity of LPOS plus carrot extract and beta
carotene against three poisonous tropical bacteria: C. jejuni, S. enteritidis, and E.
coli, (2) utilization of LPOS plus carrot extract and beta carotene in milk: full
cream and skimmed milk, and (3) utilization of LPOS plus carrot extract and beta
carotene in milk derived product: yogurt and tropical fruity milk. The
purification of LPO from bovine milk, the complimentary data analysis for
generation of LPOS antimicrobial activity by the addition of carrot extract and
beta carotene will be conducted in Japan and for the application in tropical
poisonous bacteria, milk, and milk derived product will be conducted in
Indonesia.
The outcomes of this research from three step of research are three
international publications at international‐scopus‐indexed‐journals :
International Journal of Dairy Science and one of patent: “enhanced method for
improvement of LPOS antimicrobial activity by involvement of carrot extract and
beta carotene in milk and derived product”.
Keywords: Lactoperoxidase, antimicrobial activity, carrot extract, beta carotene,
milk, derived products
INVESTIGATION DICARBONYL COMPOUNDS GENERATED FROM THE MAILLARD REACTIONS OF METHIONINE WITH REDUCING SUGARS TO ENFOLD OFF FLAVOR IN GOAT’S MILK
Population of goat in Indonesia remains low and stagnant (about 1.2 % per year). In
the other hand, Indonesia is a tropical country, which is suite for the goat’s growth.
One of strong reason is low in goat-based product’s demand since “goaty” flavor
existed in goat milk is off-flavor that is highly responsible for the consumer dislike.
Therefore, to increase the demand, the reduction of “goaty” flavor is suggested. Our
previous research showed the strategy to reduce intensity of “goaty” flavor by folding
off-flavor with maillard product. Rare sugar is the new substances beside having zero
calories and high antioxidant, but also acting as amino-binding in maillard reaction.
Among rare sugars, only three: fructose, psicose, and tagatose that able to produce
high intensity of intermediate product from maillard reaction but those intensity were
disable to enfold the “goaty” flavor. Therefore, the integration to reactive amino acid,
such as methionine is required to enhance the production intermediate product of
maillard reaction resulting in the huge suppression to off-flavor in goat’s milk.
Methionine is a remarkable reactive compound that has ability to produce high
content of carbonyl compound; an intermediate compound from maillard reaction.
Therefore, this research will integrate methionine and rare sugars in maillard reaction
process to produce high content of intermediate product of maillard reaction to
suppress the off-flavor in goat’s milk. This research will be done for 2 years and this
1
st
research will be focused for (1) production of strong carbonyl compound
(quinoxalines) from the reaction mixture containing methionine and rare sugars (2)
one patent document, (3) one research paper in Emirates Journal of Food and
Agriculture (Scopus, IF= 0.3) and (4) one proceeding’s article at International
Conference on Renewable Energy and Development, Japan (Scopus Indexed
Proceeding). To the best our knowledge, there was no research about this and this
research will be provide high value result. We believe that this research also provide a
strategy to produce the less or no goaty flavor in goat’s milk with ease.
Keywords: carbonyl compound, quinoxalines, goaty flavor, maillard reaction, rare
sugars
A point symmetry based method for transforming ODEs with three-dimensional symmetry algebras to their canonical forms
We provide an algorithmic approach to the construction of point
transformations for scalar ordinary differential equations that admit
three-dimensional symmetry algebras which lead to their respective canonical
forms
End User Adoption of ERP Systems: Investigation of Four Beliefs
This paper studies the effects of four beliefs of a group of end users on the adoption of ERP systems. The beliefs examined were perceived usefulness, ease of use, expected capability, and expected value. A sample of 58 SAP users was collected, via online survey, from a large company in Saudi Arabia. The validity and the reliability of the data collection instrument were tested using the exploratory factor analysis and Cronbach\u27s Alpha coefficients, respectively. Using multiple regression analysis, the perceived ease of use and the expected value of ERP system was found to have a significant direct effect on the acceptance of ERP system. However, the perceived usefulness and expected capability did not have a direct significant effect on acceptance of ERP systems. The study provides insights for organizations implementing ERP systems in developing countries
Fungal dysbiosis predicts the diagnosis of pediatric Crohn's disease
AIM: To investigate the accuracy of fungal dysbiosis in mucosa and stool for predicting the diagnosis of Crohn’s disease (CD). METHODS: Children were prospectively enrolled in two medical centers: one university hospital and one private gastroenterology clinic in the city of Riyadh, Kingdom of Saudi Arabia. The children with confirmed diagnosis of CD by standard guidelines were considered cases, and the others were considered non-inflammatory bowel disease controls. Mucosal and stool samples were sequenced utilizing Illumina MiSeq chemistry following the manufacturer’s protocols, and abundance and diversity of fungal taxa in mucosa and stool were analyzed. Sparse logistic regression was used to predict the diagnosis of CD. The accuracy of the classifier was tested by computing the receiver operating characteristic curves with 5-fold stratified cross-validation under 100 permutations of the training data partition and the mean area under the curve (AUC) was calculated. RESULTS: All the children were Saudi nationals. There were 15 children with CD and 20 controls. The mean age was 13.9 (range: 6.7-17.8) years for CD children and 13.9 (3.25-18.6) years for controls, and 10/15 (67%) of the CD and 13/20 (65%) of the control subjects were boys. CD locations at diagnosis were ileal (L1) in 4 and colonic (L3) in 11 children, while CD behavior was non-stricturing and non-penetrating (B1) in 12 and stricturing (B2) in 3 children. The mean AUC for the fungal dysbiosis classifier was significantly higher in stools (AUC = 0.85 ± 0.057) than in mucosa (AUC = 0.71 ± 0.067) (P < 0.001). Most fungal species were significantly more depleted in stools than mucosal samples, except for Saccharomyces cerevisiae and S. bayanus, which were significantly more abundant. Diversity was significantly more reduced in stools than in mucosa. CONCLUSION: We found high AUC of fungal dysbiosis in fecal samples of children with CD, suggesting high accuracy in predicting diagnosis of CD. Key Words: Fungiome, Mycobiome, Crohn’s disease, Inflammation, Saudi children Core tip: We found high accuracy of fungal dysbiosis in predicting diagnosis of Crohn’s disease (CD), a finding similar to bacterial dysbiosis. However, the higher area under the curve for the fungal dysbiosis classifier in stool (0.85 ± 0.057) than in mucosa (0.71 ± 0.067) (P < 0.001), contrasts with bacterial studies, suggesting higher accuracy of stool samples. Although the clinical application of this finding is limited at present by the high cost of fungal analysis, such information is important from a scientific viewpoint, to increase the understanding of the role of fungal flora in CD and to stimulate further studies.The authors extend their appreciations to the Deanship of Scientific Research at King Saud University in Riyadh, Kingdom of Saudi Arabia for funding this work through Research Group No [RGP-1436-007]. This work was also supported by a grant from the Simons Foundation [No. 409704] to Kirill Korolev) and by the startup fund from Boston University to Kirill Korolev. Simulations were carried out on Shared Computing Cluster at Boston University. Rajita Menon was partially supported by a Hariri Graduate Fellowship from Boston University. Harland Winter, MD received support from Martin Schlaff and the Diane and Dorothy Brooks Foundation. (RGP-1436-007 - King Saud University in Riyadh, Kingdom of Saudi Arabia; 409704 - Simons Foundation; Boston University; Hariri Graduate Fellowship from Boston University; Diane and Dorothy Brooks Foundation)Published versio
Writing Electronic Devices on Paper with Carbon Nanotube Ink
The normal paper used in any printer is among the cheapest flexible organic
materials that exist. We demonstrate that we can print on paper high-frequency
circuits tunable with an applied dc voltage. This is possible with the help of
an ink containing functionalized carbon nanotubes and water. After the water is
evaporated from the paper, the nanotubes remain steadily imprinted on paper,
showing a semiconducting behaviour and tunable electrical properties
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