Detection of bovine leukocyte adhesion deficiency (BLAD) in Turkish native and Holstein cattle

The purpose of this work was to study whether the bovine leukocyte adhesion deficiency ( BLAD) allele is present in native cattle breeds and the Holstein breed in Turkey. Blood samples were obtained from 120 Holstein, 20 Brown Swiss, 20 Anatolian Black, 20 Turkish Grey, 20 South Anatolian Red and 20 East Anatolian Red cattle. The isolated DNA materials were multiplied in PCR using the primer developed by Kriegesmann et al. ( 1997). In order to determine the area of mutation in PCR products, the PCR products were digested with TaqI endonuclease enzyme. The resulting fragments were analysed on 2% agarose gel for the absence of a TaqI restriction site. It was found that two of the Holstein cattle ( a bull and a cow) were heterozygote BLAD carriers. There was no homozygote BLAD animal. The BLAD allele was not found in the other breeds used in the study. The mutant BLAD allele frequency in the 120 Holstein cattle calculations was 0.0084

Determination of Pesticide Residues of Organic Wheat Flours and Some Quality Criteria of Breads

In this research, normal and sourdough breads manufactured with organic whole wheat flour, type 650 and the mixture (50%, 50%) of whole wheat flour and type 650 (w/w), were investigated. 258 Different pesticides including organic chlorinated, organic phosphorous, organic chlorinated-phosphorous, strobuline group fungicides, n-methyl carbamited, synthetic peritroit group, benzimidazole group and pyridylmethalamine group pesticides were searched as the indicator for organic flours and none of these pesticide residues were found in defined limitations. Moisture, ash, sedimentation, falling number, pharinograph, extensograph, wet and dry gluten values of organic flours were determined. Normal and sourdough breads were prepared with these flours and inner pore structure and shelf life of these breads were examined. It is determined that the bread produced with whole wheat flour was softer and the colour of this bread was dark than the others'. It is also observed that the shelf life of the bread produced with normal dough was shorter than that of sourdough bread. Additionally there was a significant breakdown on the upper side of breads that produced with normal dough

Different four PCR-multiplex systems via twenty microsatellite loci in goat

European Biotechnology Congress -- SEP 28-OCT 01, 2011 -- Istanbul, TURKEY[No Abstract Available]European Biotechnol Themat Network Asso

ASSOCIATION OF NUMBER OF ARTIFICIAL INSEMINATIONS PER PREGNANCY IN HOLSTEIN DAIRY COWS WITH POLYMORPHISM IN LUTEINIZING HORMONE RECEPTOR AND FOLLICLE STIMULATING HORMONE RECEPTOR GENES

AKSEL, ESMA GAMZE/0000-0002-0040-8933WOS: 000408149800006Failure to become pregnant is the primary reason for a dairy cow to be culled from the production herd. A cow that is cycling normally, with no reproductive abnormalities, but has failed to conceive after at least three successive inseminations may cause economic losses in dairy farms. The present study aimed to examine the association between follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHCGR) genes polymorphisms and number of artificial inseminations in the Holstein cattle breed, raised in Turkey. A total of 264 Holstein cows were included in this study, consisting of 222 cows which had undergone a low number of artificial inseminations (two or less inseminations; LI) and 42 cows with a high number of artificial inseminations (three or more inseminations; HI). The polymerase chain reaction followed by restriction fragment length polymorphism method was used to determine the FSHR-AluI and LHCGR-HhaI DNA variants. Three genotypes (CC, CG and GG) were observed for the FSHR gene in LI and HI cows. No statistical difference was found among LI and HI animals for the FSHR genotypes (P=0.934). However, only the CC genotype was detected in LI cows whereas the CC, CT and TT genotypes were detected in HI cows for the LHCGR gene. The genotype frequency of CC was found to be highest (93%) in the HI animals and an association between LHCGR genotypes and the number of artificial inseminations per pregnancy was identified (P<0.001). This is the first report to describes an association between FSHR and LHCGR polymorphisms and number of artificial inseminations in cows

A genetic probe into the ancient and medieval history of Southern Europe and West Asia

Literary and archaeological sources have preserved a rich history of Southern Europe and West Asia since the Bronze Age that can be complemented by genetics. Mycenaean period elites in Greece did not differ from the general population and included both people with some steppe ancestry and others, like the Griffin Warrior, without it. Similarly, people in the central area of the Urartian Kingdom around Lake Van lacked the steppe ancestry characteristic of the kingdom’s northern provinces. Anatolia exhibited extraordinary continuity down to the Roman and Byzantine periods, with its people serving as the demographic core of much of the Roman Empire, including the city of Rome itself. During medieval times, migrations associated with Slavic and Turkic speakers profoundly affected the region