Extract of Sesamum Indicum Seeds on the Glycogen Profile of the Liver of Adult Wistar Rat

The aim of this work was to evaluate the effect of seeds extract of Sesamum indicum on the histology liver of adult Wistar rats. Thirty Wistar rats weighing between 150-180g were divided into three groups of ten each; control group A received distilled water, experimental B and C received 200mg/kg and 400mg/kg of the seed extracts respectively. The extracts were administered for two weeks, at the end of which the animals were sacrificed; livers were removed and processed for Haematoxylin and eosin (H&E) paraffin sectioning and staining method while the serum was used for liver enzyme assay. From the results obtained, the administration of ethanolic seed extract of Sesamum indicum caused no obvious structural derangement in the organ There were no adverse effects on glycogen distribution. Keywords: Liver, Wistar rats, Sesamum indicum, glycoge

Effect of Combined Leaf Extracts of Vernonia amygdalina (Bitter Leaf) and Gongronema latifolium (Utazi) on the Pancreatic beta-Cells of Streptozotocin- Induced Diabetic Rats

The study evaluated the effect of combined extracts of Vernonia amygdalina (VA) and Gongronema latifolium (GL) on the pancreas of streptozotocin (STZ) induced diabetic Wistar rats. Thirty-two (32) albino rats were divided equally into 4 groups. Groups A and B which served as normal (NC) and diabetic (DC) controls respectively, received placebo treatment. The diabetic test groups C and D were respectively treated with combined extracts of VA and GL (200mg/kg b. w., p. o.) and insulin, (humulin 5 IU/kg, s.c.) for 28 days. Thereafter, the animals were sacrificed and blood and pancreas were collected for serum glucose and histological evaluation, respectively. Changes in animal weight were also measured within the period. From the results it was revealed that both the combined extracts and humulin significantly increased the animals’ body weight (p<0.05) from -10.5% reduction in the DC, to 7.6% and 8.9% respectively. In the same order, serum glucose significantly decreased (p<0.05) by 12.49% and 14.96% after the 28-day treatment compared to DC. The extent of reversal of hyperglycemia in the extract treated animals compared well with the insulin treated group. The biochemical results were corroborated with results of histological evaluations: The pancreatic β-cells of DC animals which were distorted and degenerated with shrunken cell mass as against prominent islet cells with normal exocrine pancreas of NC animals became rapidly proliferated upon intervention with the combined extracts, suggesting a possible regeneration of the islet cells. On the otherhand, intervention with humulin did not produce observable differences in the cyto-architecture of the pancreatic islets compared to the diabetic control, confirming an extra-pancreatic mechanism of insulin

Histomorphology and Biochemical Effects of Sida acuta leaf Extract on the Kidney of Wistar Rats

This study assessed the effect of ethanolic extract of Sida acuta leaf on the micro-anatomy of the kidney of Wistar rats. Thirty adult Wistar rats were separated into 3 groups A, B and C (n=10 per group). The Wistar rats were subjected to different doses of ethanolic extract of Sida acuta in order to evaluate the possible micro-anatomy/morphological changes in the kidney following the administration of the extract. The rats in group A were regarded as control and they received only distilled water throughout the period. The rats in group B and C were the treated groups and received ethanolic extract of Sida acuta leaves daily at doses 100 and 200mg/kgbw orally for 14 days respectively. The animals were sacrificed on the 15th day by cervical dislocation and the kidney excised. Fixed in 10% formal saline and processed for light microscopy using H&amp;E staining methods. Finding from rats’ weight that there was a significant (P&lt;0.05) decrease in body weight of the treated rats compared with the control group. The histological study revealed compressed renal corpuscle, increased bowman’s space and constricted collecting tubule which may adversely affect renal functions. Key words: Sida acuta, Kidney, histomorphology, biochemica

Rauvolfia vomitoria Afzel. disrupts dentate gyrus cells

88-94Herbal remedy for neurological problems may have adverse effects, and could prove detrimental if not regulated properly. Rauvolfia vomitoria (RV) is a herb commonly associated with psychiatry management because of its antipsychotic and sedative properties. Here, we studied the effects of the root bark extract of R. vomitoria on the dentate gyrus of adult Wistar rats. Twenty four adult Wistar rats (220 g average) were divided into four groups (n=6); control (placebo), 200, 300 and 400 mg/kg RV root bark extract, respectively for 7 days. The animals were sacrificed 24 h after last administration, and the brains were processed for histology and immunoreactivity. Results showed hypertrophy and atrophy of granule cells in all 200, 300 and 400 mg/kg RV groups, respectively. There was increased neuron specific enolase and glial fibrillary acidic protein expressions in the 200 and 300 mg/kg RV groups, while these proteins expression were decreased in the 400 mg/kg RV group. These results suggest that RV cause dentate gyrus cell injury in a dose-dependent pattern, and may lead to degeneration and disruption of functions

Comparative Study of Neuronal Degenerative Potentials of Ethanolic Root Bark and Leaf Extracts of Rauwolfia Vomitoria on the Cerebellum of Adult Wistar Rats

Rauwolfia vomitoria has been used for centuries in India and Africa for treatment of a variety of disorders including snake bites and sting, insomnia and insanity. Neuronal degenerative potentials of ethanolic root bark and leaf extracts of Rauwolfia vomitoria on the cerebellum of adult wistar rats was investigated. Thirty wistar rats weighing between 170-240g were divided into six groups, each consisting of five rats. Groups A served as the normal control that received distilled water while group B served as the olive oil control that received 0.5mls of olive oil. Experimental groups C and D received 200mg/kg, 300mg/kg of ethanolic root bark extracts while groups E and F received 200mg/kg, 300mg/kg of the leaf extract orally respectively for seven days.  In this study, the treatment groups showed a dose-dependent degree of silver impregnation of the cell bodies and axons. The sections of the cerebellar cortex of the treated group C, D, E and F showed various degrees of neurodegenerative changes highlighted by the silver stain impregnation which was more intense in groups C and D that received 200mg/kg and 300mg/kg ethanolic root-bark than in the groups E and F that received 200mg/kg and 300mg/kg of ethanolic leaf extract. These changes may result in cerebellar dysfunction Keywords: Ethanolic extracts, Root-bark extract, Leaf extract, Rauwolfia vomitoria

Gonadal histo-morphologies and serum hormonal milieu in female rats treated with azadirachta indica leaf extract

Background: azadirachta indica is a tree with most of the parts having various medicinal values. It is however popular because of its high potencies, as antimalarial and anti-fertility agents, which the locals still exploit. Objective: We investigated the effect of the methanol leaf extract on the serum levels of the pituitary-gonad hormones and the histo-morphology of the ovary and uterus of adult female rats. Materials and Methods: Eighteen adult female Wistar rats were divided into three groups (A, B and C) of six animals each. Group A was the control group that received distilled water orally, while groups B and C were the experimental groups that received 200mg/kg and 400mg/kg of the extract respectively by oral intubation for fourteen days. The animals were sacrificed on the fifteenth day, and blood was collected from the left ventricles of the hearts and subsequently spurned in heparinized bottles for serum hormonal assay. The ovaries and the uteri were then dissected out and preserved in Bouin’s fluid. Routine haematoxylin and eosin method was used to stain them. Results: There were significant (p<0.0001) lower serum levels of luteinizing hormone (LH) in the treatment groups, especially in the 400mg/kg group, while there were significant (p<0.0001) higher progesterone (PH) levels in the treatment groups. The follicle stimulating hormone (FSH) levels were however not different (p=0.0502) from the control. The histo-morphologic studies revealed no obvious pathological changes in the ovaries and uteri of the treatment groups. Conclusion: 200mg/kg and 400mg/kg of methanol extract of the leaf of A. indica does not have any obvious effect on the histo-morphologies of the ovary and uterus, but showed significant changes in the serum levels of LH and PH of female Wistar rat, implying that the effect of the extract may have been at a level other than these organs of study

Teratogenic Effects of Crude Ethanolic Root Bark and Leaf Extracts of Rauwolfia vomitoria (Apocynaceae) on the Femur of Albino Wistar Rat Fetuses

Introduction. Rauwolfia vomitoria is a plant used as a sedative and in the treatment of psychotic tendency. This study was on the teratogenic effects of its root bark and leaf extracts on Wistar rat’s fetal femurs. Materials and Methods. Twenty-five female rats weighing between 180 and 200 g were divided into 5 groups, of 5 rats each. Group A was the control, while Groups B, C, D, and E were the experimental. The female rats were mated with mature male rats to allow for pregnancy. Groups B and C animals received orally 150 mg/kg each of the root bark and leaf extracts of Rauwolfia vomitoria, respectively, while Groups D and E animals received 250 mg/kg bodyweight each of the root bark and leaf extracts of Rauwolfia vomitoria, respectively, from day 7 to day 11 of gestation. On day 20 of gestation, the rats were sacrificed, the fetuses were examined, and their femurs were dissected out and preserved, decalcified, and routinely processed using the Haematoxylin and Eosin staining method. Results. Histological observations of the fetal femur bones showed numerous osteoblast and osteoclast, hypertrophy, and hyperplasia of bone cells compared with the control. Conclusion. Ethanolic root bark and leaf extracts of Rauwolfia vomitoria may lead to advanced skeletal development