Altered distribution of plasma PAF-AH between HDLs and other lipoproteins in hyperlipidemia and diabetes mellitus

Platelet-activating factor acetylhydrolase (PAF-AH) is a phospholipase A2 associated with lipoproteins that hydrolyzes platelet-activating factor (PAF) and oxidized phospholipids. We have developed an ELISA for PAF-AH that is more sensitive than previous methods, and have quantified HDL-associated and non-HDL-associated PAF-AH in healthy, hyperlipidemic, and diabetic subjects. In healthy subjects, plasma total PAF-AH concentration was positively correlated with PAF-AH activity and with plasma total cholesterol, triacylglycerol, LDL cholesterol and apolipoprotein B (apoB) concentrations (all P < 0.01). HDL-associated PAF-AH concentration was correlated positively with plasma apoA-I and HDL cholesterol. Subjects with hyperlipidemia (n = 73) and diabetes mellitus (n = 87) had higher HDL-associated PAF-AH concentrations than did controls (P < 0.01). Non-HDL-associated PAF-AH concentration was lower in diabetic subjects than in controls (P < 0.01). Both hyperlipidemic and diabetic subjects had lower ratios of PAF-AH to apoB (P < 0.01) and higher ratios of PAF-AH to apoA-I (P < 0.01) than did controls. Our results show that the distribution of PAF-AH mass between HDLs and LDLs is determined partly by the concentrations of the lipoproteins and partly by the mass of enzyme per lipoprotein particle, which is disturbed in hyperlipidemia and diabetes mellitus

PETREL: Platform for Extra and Terrestrial Remote Examination with LCTF

A small satellite ”PETREL” for UV astronomy and remote sensing with ”tunable” multi-spectral cameras conducted by an academia-industrial collaboration is presented. This project was originally proposed by an astronomer who desired a satellite for exploration of explosive objects in ultraviolet. To avoid the earthshine the astronomical observations are scheduled only in the nighttime. To utilize the daytime more electively we conceived a plan of ”satellite sharing” with the industrial collaborators, that can also reduce the developing cost drastically. The daytime mission is spectroscopy that is one of the potential fields in terms of data business, because that can provide chemical and biological information on the surface of the earth. We employ multi-spectral cameras making use of liquid crystal tunable filters (LCTFs) that enable adaptive observations at the optimized wave-bands for each targets. In 2020, this remote-sensing project and ultraviolet astronomy mission were accepted as a small satellite project of JAXA’s Innovative Satellite Technology Demonstration program and as an ISAS/JAXA’s small-scale program, respectively. This satellit

Search for gravitational-lensing signatures in the full third observing run of the LIGO-Virgo network

Gravitational lensing by massive objects along the line of sight to the source causes distortions of gravitational wave-signals; such distortions may reveal information about fundamental physics, cosmology and astrophysics. In this work, we have extended the search for lensing signatures to all binary black hole events from the third observing run of the LIGO--Virgo network. We search for repeated signals from strong lensing by 1) performing targeted searches for subthreshold signals, 2) calculating the degree of overlap amongst the intrinsic parameters and sky location of pairs of signals, 3) comparing the similarities of the spectrograms amongst pairs of signals, and 4) performing dual-signal Bayesian analysis that takes into account selection effects and astrophysical knowledge. We also search for distortions to the gravitational waveform caused by 1) frequency-independent phase shifts in strongly lensed images, and 2) frequency-dependent modulation of the amplitude and phase due to point masses. None of these searches yields significant evidence for lensing. Finally, we use the non-detection of gravitational-wave lensing to constrain the lensing rate based on the latest merger-rate estimates and the fraction of dark matter composed of compact objects

Characteristic cardiovascular manifestation in homozygous and heterozygous familial hypercholesterolemia

Background The aortic valve dysfunction of patients with homozygous familial hypercholesterolemia (FH) suggests that hypercholesterolemia affects not only coronary arteries, but also the aortic valve. We studied the aortic root of patients with homozygous FH and those of heterozygous FH to characterize the premature atherosclerotic lesions, using histopathological specimens. Methods and results The aortic roots of 10 homozygous FH patients, aged 9 to 58 years, were studied by cardiac catheterization with several angiographies. The aortic root of 39 heterozygous FH patients under age 60 were also examined for aortic and mitral valvular functions by color Doppler echocardiography, and 30 normocholesterolemic patients with coronary artery disease were examined as controls. In addition, in 22 FH and 20 control subjects, the internal diameter of the aortic annulus and the aortic ridge in cardiac cycles were measured. Of the 10 FH homozygotes, 8 patients had aortic regurgitation demonstrated by aortography; three of them showed significant transvalvular pressure gradients. Stenotic changes of coronary ostia were observed in 8 of the 10 homozygotes with moderate coronary atherosclerosis. Of the 39 FH heterozygotes, ten patients had aortic regurgitation shown by Doppler echocardiography, as did only one of the 30 control subjects (P<0.05). The average diameter and distensibility of the ascending aorta were significantly reduced in the heterozygotes compared to the control subjects. The surgically resected cusp specimens of aortic valves obtained from one homozygous and one heterozygous patient showed significant thickening of the cusp with foam cell infiltration. Conclusions Premature atherosclerosis in FH had a characteristic distribution, affecting the aortic root dominantly. The involvement of the aortic valve indicating “hypercholesterolemic valvulopathy” was a peculiar feature of FH, especially its homozygous form, but was reminiscent of ubiquitous processes due to hypercholesterolemia