Reproduction of Array Observation Records by Means of Centrifuge Shaking Table Model

This paper shows the effects of the degree of consolidation of the soft clay layer on the strong motion response. Seismic behavior of the Kobe artificial islands during the 1995 Hyogo-ken Nambu earthquake is studied by using centrifuge shaking table test. At the earthquake, it is known that the liquefaction damage of artificial island was different from each other. Authors consider the reason why is due to the degree of consolidation of clay layer underlying the reclaimed ground. The model grounds used for the centrifuge test are made by the clay and fill material sampled from Kobe artificial island, and each clay layer of models is consolidated as the same degree as the sites. First, from the viewpoint of the reproducibility of in-situ behavior, the seismic response and the ground settlement are compared with observation data. Next, we compare the seismic response of the test results of the different degree of consolidation. It is found that the degree of consolidation and the shear strength of the clay layer significantly affect the ground behavior. The large damage is not always come to being on the ground with soft clay layer

Solid state diffusion bonding of doped tungsten alloys with different thermo-mechanical properties

To develop joints using W materials with different thermo-mechanical properties, solid state diffusion bonding involving two different W materials (pure W, K-doped W, or K-doped W-3%Re) and using a pure V interlayer (1.5 mm, 0.5 mm, or 0.05 mm thick) were carried out at 1250 °C for 1 h. The use of a thin interlayer was found to be effective from the point of optimizing the strength and thermal diffusivity. Diffusion bonding at lower temperatures or utilizing W materials with higher recrystallization temperatures were also determined to be effective because pure W can recrystallize at 1250 °C. Further evaluation of a wide range of interlayer thicknesses and thermo-mechanical test conditions is necessary based on the present work to obtain optimum W/V/W joints

The double-stranded break-forming activity of plant SPO11s and a novel rice SPO11 revealed by a Drosophila bioassay

<p>Abstract</p> <p>Background</p> <p>SPO11 is a key protein for promoting meiotic recombination, by generating chromatin locus- and timing-specific DNA double-strand breaks (DSBs). The DSB activity of SPO11 was shown by genetic analyses, but whether SPO11 exerts DSB-forming activity by itself is still an unanswered question. DSB formation by SPO11 has not been detected by biochemical means, probably because of a lack of proper protein-folding, posttranslational modifications, and/or specific SPO11-interacting proteins required for this activity. In addition, plants have multiple SPO11-homologues.</p> <p>Results</p> <p>To determine whether SPO11 can cleave DNA by itself, and to identify which plant SPO11 homologue cleaves DNA, we developed a <it>Drosophila </it>bioassay system that detects the DSB signals generated by a plant SPO11 homologue expressed ectopically. We cytologically and genetically demonstrated the DSB activities of <it>Arabidopsis </it>AtSPO11-1 and AtSPO11-2, which are required for meiosis, in the absence of other plant proteins. Using this bioassay, we further found that a novel SPO11-homologue, OsSPO11D, which has no counterpart in <it>Arabidopsis</it>, displays prominent DSB-forming activity. Quantitative analyses of the rice SPO11 transcripts revealed the specific increase in OsSPO11D mRNA in the anthers containing meiotic pollen mother cells.</p> <p>Conclusions</p> <p>The <it>Drosophila </it>bioassay system successfully demonstrated that some plant SPO11 orthologues have intrinsic DSB activities. Furthermore, we identified a novel SPO11 homologue, OsSPO11D, with robust DSB activity and a possible meiotic function.</p

Compression induced shear damage in brittle solids by scattered microcracking

Failure observation and numerical analysis were conducted to understand how shear damage develops in brittle solids under biaxial compression. A biaxial compression often induces shear damaged in brittle solids, which is preceded by a formation of huge number of array cracks. Cracks in the array appeared gradually as applied compression increased. They were almost similar in shape; gently curved but were possible to approximate a troop of straight cracks almost parallel to each other without loss of essential characteristics. Under a uniaxial compression, a brittle material tends to fail exhibiting a crack growth almost parallel to the loading axis. In this situation, the crack propagates rather in a stable fashion since the stress intensity factor at crack tip generally decreases with crack extension. Under a biaxial compression, however, such a stable crack growth is strongly inhibited. Consequently, an array of microcracks often appears as a presage of the macroscopic shear failure. A mechanism of the appearance of damaged zone with increase of applied compression was discussed using a scattered cracking model. It was found that each crack composing the damaged zone has a possibility to open due to crack-to-crack interaction and a localized tensile stress appeared both in the interior and in the exterior of the damaged zone. The localized tension appeared in the interior of the damaged zone may increase a crack density, while that appeared in the exterior of the damaged zone would bring an enlargement of the damaged zone

Composite structural motifs of binding sites for delineating biological functions of proteins

Most biological processes are described as a series of interactions between proteins and other molecules, and interactions are in turn described in terms of atomic structures. To annotate protein functions as sets of interaction states at atomic resolution, and thereby to better understand the relation between protein interactions and biological functions, we conducted exhaustive all-against-all atomic structure comparisons of all known binding sites for ligands including small molecules, proteins and nucleic acids, and identified recurring elementary motifs. By integrating the elementary motifs associated with each subunit, we defined composite motifs which represent context-dependent combinations of elementary motifs. It is demonstrated that function similarity can be better inferred from composite motif similarity compared to the similarity of protein sequences or of individual binding sites. By integrating the composite motifs associated with each protein function, we define meta-composite motifs each of which is regarded as a time-independent diagrammatic representation of a biological process. It is shown that meta-composite motifs provide richer annotations of biological processes than sequence clusters. The present results serve as a basis for bridging atomic structures to higher-order biological phenomena by classification and integration of binding site structures.Comment: 34 pages, 7 figure

Emerging concepts in biomarker discovery; The US-Japan workshop on immunological molecular markers in oncology

Supported by the Office of International Affairs, National Cancer Institute (NCI), the "US-Japan Workshop on Immunological Biomarkers in Oncology" was held in March 2009. The workshop was related to a task force launched by the International Society for the Biological Therapy of Cancer (iSBTc) and the United States Food and Drug Administration (FDA) to identify strategies for biomarker discovery and validation in the field of biotherapy. The effort will culminate on October 28th 2009 in the "iSBTc-FDA-NCI Workshop on Prognostic and Predictive Immunologic Biomarkers in Cancer", which will be held in Washington DC in association with the Annual Meeting. The purposes of the US-Japan workshop were a) to discuss novel approaches to enhance the discovery of predictive and/or prognostic markers in cancer immunotherapy; b) to define the state of the science in biomarker discovery and validation. The participation of Japanese and US scientists provided the opportunity to identify shared or discordant themes across the distinct immune genetic background and the diverse prevalence of disease between the two Nations