SAMP8 マウスの連鎖解析による記憶学習障害候補責任遺伝子の同定

富山大学・富生命博甲第60号・AKBOR MARUF MOHAMMAD・2014/03/21・★論文非公開★富山大

Dentin Phosphophoryn Promotes Odontoblast Differentiation in vitro and Induction of Mineralized Matrix in vivo.

北海道医療大学平成25年

Effects of Amelogenin on Proliferation, Differentiation, and Mineralization of Rat Bone Marrow Mesenchymal Stem Cells In Vitro

The aim of this study was to clarify the function of amelogenin, the major protein of enamel matrix derivative, on the proliferation, differentiation, and mineralization of cultured rat bone marrow stem cells (BMSCs), toward the establishment of future bone regenerative therapies. No differences in the morphology of BMSCs or in cell numbers were found between amelogenin addition and additive-free groups. The promotion of ALPase activity and the formation of mineralized nodules were detected at an early stage in amelogenin addition group. In quantitative real-time RT-PCR, mRNA expression of osteopontin, osteonectin, and type I collagen was promoted for 0.5 hours and 24 hours by addition of amelogenin. The mRNA expression of osteocalcin and DMP-1 was also stimulated for 24 hours and 0.5 hours, respectively, in amelogenin addition group. These findings clearly indicate that amelogenin promoted the differentiation and mineralization of rat BMSCs but did not affect cell proliferation or cell morphology

Bioequivalence evaluation of two capsule formulations of amoxicillin in healthy adult male bangladeshi volunteers: A single-dose, randomized, open-label, two-period crossover study

AbstractBackground: Amoxicillin, a semisynthetic penicillin antibiotic, is widely prescribed in Bangladesh due to its extended spectrum and its rapid and extensive oral absorption with good tolerability. Although a number of generic oral formulations of amoxicillin are available in Bangladesh, a study of the bioequivalence and pharmacokinetic properties of these formulations has not yet been conducted in a Bangladeshi population.Objective: The aim of this study was to assess the pharmacokinetic properties and bioequivalence of 2 formulations of amoxicillin 500-mg capsules (test, SK-mox®; reference, Amoxil-Bencard®) using serum data.Methods: This single-dose, randomized, open-label, 2-period crossover study was conducted in healthy male subjects in compliance with the Declaration of Helsinki and International Conference on Harmonisation guidelines. Subjects were assigned to receive the test or the reference drug as a single-dose, 500-mg capsule under fasting conditions after a 1-week washout period. After oral administration, blood samples were collected and analyzed for amoxicillin concentration using a validated high-performance liquid chromatography method. The pharmacokinetic parameters were determined using a noncompartmental method. The formulations were considered bioequivalent if the natural log-transformed ratios of pharmacokinetic parameters were within the predetermined equivalence range of 80% to 125%, according to the US Food and Drug Administration (FDA) requirement.Results: Twenty-four healthy adult male Bangladeshi volunteers (mean [SD] age, 26.92 [3.37] years; age range, 23–34 years; mean [SD] body mass index, 23.O9 [1.58] kg/m2) participated in the study. Using serum data, the values obtained for the test and reference formulations, respectively, were as follows: Cmax, 9.85 (2.73) and 10.63 (2.12) μg/mL; Tmax, 1.29 (0.58) and 1.33 (0.49) hours; and AUC0–12, 27.09 (7.62) and 28.56 (6.30) μg/mL · h−1. No period, sequence, or formulation effects were observed; however, significant variation was found among subjects with regard to AUC0–12 (P < 0.001), AUC0−∞ (P = 0.002), area under the moment curve (AUMC) from 0 to 12 hours (P < 0.001), and AUMC0−∞ (P = 0.017). All CIs for the parameters measured were within the FDA-accepted limits of 80% to 125%.Conclusion: The present study suggests that the test 500-mg amoxicillin capsule was bioequivalent to the reference 500-mg capsule according to the FDA regulatory definition, in this population of healthy adult male Bangladeshi volunteers

ラット上顎臼歯におけるBMP-石灰藻由来ハイドロキシアパタイト複合体の修復象牙質誘導

rhBMP(骨形成タンパク質)v-FRIOS Algipore(石灰藻由来の多孔質ハイドロキシアパタイト)複合体がラットを用いた直接覆髄実験において効果的に良質な修復象牙質形成を誘導するか検討した。BMP-HA群は、HA群、水酸化カルシウム群に比べて早期から旺盛な修復象牙質形成を認め、細管構造を有する緻密な構造であった。BMP-HA群では早期から炎症が軽度で、全実験期間を通して他の実験群に比べて歯髄の炎症の程度は軽度であった。水酸化カルシウム製剤によって誘導された修復象牙質は全体的に多孔質で、トンネル状の欠陥が存在し、全ての観察期間を通して歯髄に炎症反応を認めた。歯髄保存療法剤としてのrhBMPv-FRIOS Algipore複合体の有効性が示唆された

Leather Industry in Bangladesh: A Systematic Literature Review

There has been a moderate number of research works, published and unpublished, relating to the broader field of leather industry in Bangladesh. Research on and for the leather industry has undoubtedly grown-up in contemporary years. The existing socio-economic condition of Bangladesh provides an encouraging prospect for leather Industry. It attracted many economists, environmentalists and technical persons to do research works regarding various aspects of this sector. This study makes a humble attempt to present a brief review of the existing relevant literature on the leather industry of Bangladesh

Adesão às recomendações de boas práticas em insulinoterapia: relação com o controle glicêmico

Objective: To analyze the relation between adherence levels to good practice recommendations for insulin therapy and glycemic control metrics in patients with diabetes mellitus. Methods: A descriptive, cross-sectional, quantitative study was conducted with 102 patients with diabetes mellitus. Data were collected by means of semi structured interviews and complemented by information obtained from medical records. Participants answered four collection instruments: I) a sociodemographic and clinical characterization form, II) a reminder of insulin therapy guidelines, III) a capillary blood glucose self-monitoring record sheet and IV) a record form of glycemic control assessment metrics. Results: Results showed a statistically significant association between non-adherence level (100%) to insulin therapy recommendations with estimated time on target ≤ 70% and between non-adherence (80%) and standard deviation ≥ 50 mg/dl. Conclusion: These findings corroborate that non adherence to good insulin therapy recommendations contribute to a lack of glycemic control.Objetivo: evaluar la relación entre los niveles de adherencia a las recomendaciones de buenas prácticas en insulinoterapia y las métricas de control glucémico en pacientes con diabetes mellitus. Métodos: estudio descriptivo, transversal y cuantitativo, realizado con 102 pacientes con diabetes mellitus. La recolección de datos ocurrió a través de entrevistas semiestructuradas y, de forma complementaria, con datos obtenidos de las historias clínicas. Se aplicaron cuatro instrumentos de recolección: I) ficha de caracterización sociodemográfica y clínica, II) recordatorio de pautas de insulinoterapia, III) ficha de autocontrol de glucemia capilar y IV) ficha de registro de métricas de evaluación del control glucémico. Resultados: hubo una asociación estadísticamente significativa entre el nivel de no adherencia (100%) a las recomendaciones de insulinoterapia con el tiempo estimado en el objetivo ≤ 70% y entre (80%) la no adherencia y la desviación estándar ≥ 50 mg/dl. Conclusión: estos hallazgos validan que la no adherencia a las recomendaciones de buenas prácticas de insulinoterapia contribuye para la falta de control glucémico. Descriptores: Adherencia y cumplimiento terapéutico; recomendaciones de buenas prácticas; terapia con insulina; Control Glucémico.Objetivo: Analisar a relação entre os níveis de adesão às recomendações de boas práticas em insulinoterapia e as métricas de controle glicêmico em pacientes com diabetes mellitus. Métodos: Estudo descritivo, transversal e quantitativo, realizado com 102 pacientes com diabetes mellitus. A coleta de dados ocorreu por meio de entrevista semiestruturada e, em caráter complementar, com dados obtidos dos prontuários. Aplicaram-se quatro instrumentos de coleta: I) formulário de caracterização sociodemográfica e clínica, II)  recordatório de orientações sobre insulinoterapia, III)  folha de registro da automonitorização da glicemia capilar e IV) formulário de registro das métricas de avaliação do controle glicêmico. Resultados: Houve associação estatística significativa entre nível de não adesão (100%) às recomendações em insulinoterapia e tempo no alvo estimado ≤ 70%, assim como entre 80% de não adesão e desvio padrão ≥ 50 mg/dl. Conclusão: Esses achados validam que a não adesão às recomendações de boas práticas de insulinoterapia contribui para o descontrole glicêmico

Effect of curcumin and gliotoxin on rat liver myofibroblasts culture

Since 1990s, when it was demonstrated by Hammel and others, that liver fibrosis is reversible, researchers and physicians actively search for new antifibrotic therapies. In recent years knowledge of liver fibrosis pathophysiology has greatly advanced and new cellular and molecular mechanisms were described. The cells that determine extracellular matrix components distribution are myofibroblasts, but their origin is diverse. They can be activated hepatic stellate cells (HSC), portal fibroblasts (PF) or circulating mesenchymal stem cells of bone marrow. Activation of HSC and PF and their fibrogenic potential is upregulated by transcription factor NF-B. Among large number of substrates to inhibit NF-B and induce apoptosis we chose curcumin and gliotoxin. Primarily in current work we optimized the explantation culture method for isolation of hepatic myofibroblasts and received two different cultures - myofibroblasts of HSC and PF origin. Exposition of 50 ?M curcumin and 0,1 ?M gliotoxi

Distribution, sources, and pollution levels of toxic metal(loid)s in an urban river (Ichamati), Bangladesh using SOM and PMF modeling with GIS tool

Indexical assessment coupled with a self-organizing map (SOM) and positive matrix factorization (PMF) modeling of toxic metal(loid)s in sediment and water of the aquatic environment provides valuable information from the environmental management perspective. However, in northwest Bangladesh, indexical and modeling assessments of toxic metal(loid)s in surface water and sediment are still rare. Toxic metal(loid)s were measured in sediment and surface water from an urban polluted river (Ichamati) in northwest Bangladesh using an atomic absorption spectrophotometer to assess distribution, pollution levels, sources, and potential environmental risks to the aquatic environment. The mean concentrations (mg/kg) of metal(loid)s in water are as follows: Fe (871) > Mn (382) > Cr (72.4) > Zn (34.2) > Co (20.8) > Pb (17.6) > Ni (16.7) > Ag (14.9) > As (9.0) > Cu (5.63) > Cd (2.65), while in sediment, the concentration follows the order, Fe (18,725) > Mn (551) > Zn (213) > Cu (47.6) > Cr (30.2) > Ni (24.2) > Pb (23.8) > Co (9.61) > As (8.23) > Cd (0.80) > Ag (0.60). All metal concentrations were within standard guideline values except for Cr and Pb for water and Cd, Zn, Cu, Pb, and As for sediment. The outcomes of eco-environmental indices, including contamination and enrichment factors and geo-accumulation index, differed spatially, indicating that most of the sediment sites were moderately to highly polluted by Cd, Zn, and As. Cd and Zn content can trigger ecological risks. The positive matrix factorization (PMF) model recognized three probable sources of sediment, i.e., natural source (49.39%), industrial pollution (19.72%), and agricultural source (30.92%), and three possible sources of water, i.e., geogenic source (45.41%), industrial pollution (22.88%), and industrial point source (31.72%), respectively. SOM analysis identified four spatial patterns, e.g., Fe–Mn-Ag, Cd–Cu, Cr-Pb-As-Ni, and Zn–Co in water and three patterns, e.g., Mn-Co–Ni-Cr, Cd-Cu-Pb–Zn, and As-Fe-Ag in sediment. The spatial distribution of entropy water quality index values shows that the southwestern area possesses “poor” quality water. Overall, the levels of metal(loid) pollution in the investigated river surpassed a critical threshold, which might have serious consequences for the river’s aquatic biota and human health in the long run