Comparative evaluation of antioxidant power of hydroacoholic extract of Terminalia bellerica and Emblica officinalis plant

The present study was aimed to evaluate antioxidant activity in hydroalcoholic extract of fruits of Terminalia chebula and Emblica officinalis. Antioxidant activity of the extracts was determined using diphenylpicrylhydrazyl (DPPH), NO and Total antioxidant assays. Extracts of both medicinal plant exhibited antioxidant potential but E. officinalis proved more active. The presence of antioxidant activity in the extracts showed that these plants have the potential to be an alternate source of natural antioxidants. In vivo study is needed for successful commercialization and to benefit the food and pharmaceutical industries

AN OPEN LABELLED RANDOMISED CLINICAL TRIAL ON EFFICACY OF HARITAKI MODAKA AND ABHIJEET TAILA IN THE MANAGEMENT OF COMPUTER VISION SYNDROME

Computer Vision Syndrome is the new nomenclature to the visual, ocular, and systemic symptoms arising due to the long time and improper working on the computer. Computer Vision Syndrome is a repetitive strain disorder as defined by the American Optometric. With progressive increase in the usage of computers, the term Repeated Stress Injury has found a place in contemporary medical science. The ocular complaints experienced by computer users typically include eyestrain, eye fatigue, burning sensations, irritation, redness, blurred vision, and dry eyes, among others. This group of symptoms is called Asthenopia. The incidence of Computer Vision Syndrome is as high as 50% to 90% among the employees of computer occupation. The causes for the inefficiencies and the visual symptoms are a combination of individual visual problems and poor office ergonomics. Material & Methods: In this clinical study on CVS, 60 patients were registered from Shalakya tantra OPD, and all of them completed the treatment. In Group A, 30 patients were prescribed Abhijeet Taila for Pratimarsha Nasya and in Group B, 30 patients were prescribed Haritaki Modak tablets internally and Abhijeet Taila for Pratimarsha Nasya. Results: In total, 20% and 33.33% patients got completely cured in Group A and B respectively. Discussion: Both the drugs were effective in managing the condition, but it was observed they were more effective when given together. Multi-centric studies with larger sample size on the same drugs should be carried out to evaluate the effect of the drugs and, also so that the observations can be noted well and can be better suggested

Conditions associated with discarding of blood and its components in a blood bank of tertiary care hospital of Western Uttar Pradesh: a retrospective study

Background: Blood transfusion services in India rely on different factors for a smooth workout. Thus proper utilization of blood is necessary with minimal wasting. The aim was to evaluate the causes of discarding of blood and its components and to implement possible intervention for minimizing wastage. Methods: A retrospective study was carried out in the blood bank of Sardar Vallabh Bhai Patel Hospital, Lala Lajpat Rai Medical College, Meerut, Uttar Pradesh over a time period of 24 months from April 2019 to March 2021. Results: A total of 21627 units were collected from donors. A total of 11534 whole blood (WB), 10093 packed red blood cells (PRBC), 3245 platelets (PC), and 9669 fresh frozen plasma (FFP) were prepared. A total of 4046 units of blood and its components were discarded. The discard rate for WB, PRBC, PC and FFP was 6.25%, 7.46%, 38.39%, and 13.71% respectively. Conclusions: An average of 18.70% of blood units were discarded during this study period. The overall most common reason for discard was the date of expiry followed by transfusion transmitted infections (TTI). To minimize wastage of blood units, blood banks should be fully committed to organizing and coordinating the blood transfusion services,implementing all possible strategies as discussed in order to lower the discarding of blood and its units and to make them widely available

DISCRIMINANT ANALYSIS OF PURIFICATION ON TURMERIC (CURCUMA LONGA LINN) SAMPLES BASED ON PHYTO-PHARMACOGNOSTICAL AND MULTIVARIATE CHEMOMETRIC TECHNIQUE

Objective: To establish an acceptable identification system of various purification effects in context classical based different media on turmeric samples and relates its altering pattern in favor of phyto-pharmacognostical, image processing and multivariate chemometric analysis.Methods: Authenticated turmeric samples purified through different processes by using different media such as cow's urine, panchapllava (five different plants tender leaves), the inflorescence of alambusha (Sphaeranthus indicus, Linn) decoction, water and buttermilk. Resultant samples dried, pulverized and undertaken powder microscopy, image processing, physicochemical and chromatographic fingerprinting (HPTLC). The multivariate chemometric analysis, principal component analysis (PCA) analyzed with help of Unscrambler and image processing in Matlab software.Results: The addition of characters of medias drug with turmeric powders like the crystal of gomutra, pollen grain and starch grain of Alambusha, epidermis, fibre, the crystal of panchapllava. Identify different perceivable colors in variously processed turmeric by analyzing the Lab color space through the Image segmentation. PC1 and PC2 explained (90 + 9) % total variance in score plot of respective purify turmeric samples shown clear grouping in relation to the physicochemical constant. Quantification of curcumin in various treated turmeric samples displayed variation due to additive effect in high-performance thin layer chromatographic profile.Conclusion: This study proved that purification in ayurveda not only refers to the elimination of toxins and unwanted particles but also the transformation in the properties in the primary substance rendering it safe as well as many desired qualities are imbibed in it

Biodegradable PEG-PCL Nanoparticles for Co-delivery of MUC1 Inhibitor and Doxorubicin for the Confinement of Triple-Negative Breast Cancer

Combating triple-negative breast cancer (TNBC) is still a problem, despite the development of numerous drug delivery approaches. Mucin1 (MUC1), a glycoprotein linked to chemo-resistance and progressive malignancy, is unregulated in TNBC. GO-201, a MUC1 peptide inhibitor that impairs MUC1 activity, promotes necrotic cell death by binding to the MUC1-C unit. The current study deals with the synthesis and development of a novel nano-formulation (DM-PEG-PCL NPs) comprising of polyethylene glycol-polycaprolactone (PEG-PCL) polymer loaded with MUC1 inhibitor and an effective anticancer drug, doxorubicin (DOX). The DOX and MUC1 loaded nanoparticles were fully characterized, and their different physicochemical properties, viz. size, shape, surface charge, entrapment efficiencies, release behavior, etc., were determined. With IC(50) values of 5.8 and 2.4 nm on breast cancer cell lines, accordingly, and a combination index (CI) of < 1.0, DM-PEG-PCL NPs displayed enhanced toxicity towards breast cancer cells (MCF-7 and MDA-MB-231) than DOX-PEG-PCL and MUC1i-PEG-PCL nanoparticles. Fluorescence microscopy analysis revealed DOX localization in the nucleus and MUC1 inhibitor in the mitochondria. Further, DM-PEG-PCL NPs treated breast cancer cells showed increased mitochondrial damage with enhancement in caspase-3 expression and reduction in Bcl-2 expression.In vivo evaluation using Ehrlich Ascites Carcinoma bearing mice explicitly stated that DM-PEG-PCL NPs therapy minimized tumor growth relative to control treatment. Further, acute toxicity studies did not reveal any adverse effects on organs and their functions, as no mortalities were observed. The current research reports for the first time the synergistic approach of combination entrapment of a clinical chemotherapeutic (DOX) and an anticancer peptide (MUC1 inhibitor) encased in a diblock PEG-PCL copolymer. Incorporating both DOX and MUC1 inhibitors in PEG-PCL NPs in the designed nanoformulation has provided chances and insights for treating triple-negative breast tumors. Our controlled delivery technology is biodegradable, non-toxic, and anti-multidrug-resistant. In addition, this tailored smart nanoformulation has been particularly effective in the therapy of triple-negative breast cancer. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10924-022-02654-4

To Study effect of external cephalic version in breech pregnancy with respect to antenatal patient admitted in M.Y. Hospital, Indore

Background &amp; Method: An interventional prospective study was conducted in Department of Obstetrics and Gynecology at M.G.M. Medical College and M.Y. Hospital Indore in women with breech pregnancy admitted, Minimum of 30 cases were screened by ultrasound, then presentation were confirmed. All the patients who are diagnosed with breech adviced to go for USG with colour doppler at term and their follow up till they deliver were recorded. Result: Total number of breech admitted was 679 out of which maximum in the age group of 22-26 i.e 49.6%. Maximum number of women 407(60%) were found be unbooked and 272 (40%) of women are booked. Total no. of deliveries and breech admitted in the hospital, in study period total no. of cases delivered during the study time found to be 17,308 out of which 679 patient were breech, so incidence of breech found to be 3.9 %. Among them 30 patient give consent for ECV. Conclusion: It was concluded that External Cephalic Version is a valuable though under used option in the management of breech presentation at term. It is a relatively safe procedure, complications were very rare, it is simple to learn and perform. Vigilance for searching breech presentation and counselling after 36 weeks is important. A proper understanding of the risk is essential for the obstetrician to allow accurate counselling. Breech presentation is associated with higher incidence of mortality and morbidity irrespective of the route of delivery as compared to cephalic presentation. Any complications associated with breech presentation can be reduced by converting it to cephalic presentation by ECV. Keywords: External, Cephalic, Pregnancy &amp; Antenatal

Biofilm-<i>i</i>: A Platform for Predicting Biofilm Inhibitors Using Quantitative Structure—Relationship (QSAR) Based Regression Models to Curb Antibiotic Resistance

Antibiotic drug resistance has emerged as a major public health threat globally. One of the leading causes of drug resistance is the colonization of microorganisms in biofilm mode. Hence, there is an urgent need to design novel and highly effective biofilm inhibitors that can work either synergistically with antibiotics or individually. Therefore, we have developed a recursive regression-based platform “Biofilm-i” employing a quantitative structure–activity relationship approach for making generalized predictions, along with group and species-specific predictions of biofilm inhibition efficiency of chemical(s). The platform encompasses eight predictors, three analysis tools, and data visualization modules. The experimentally validated biofilm inhibitors for model development were retrieved from the “aBiofilm” resource and processed using a 10-fold cross-validation approach using the support vector machine and andom forest machine learning techniques. The data was further sub-divided into training/testing and independent validation sets. From training/testing data sets the Pearson’s correlation coefficient of overall chemicals, Gram-positive bacteria, Gram-negative bacteria, fungus, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, and Escherichia coli was 0.60, 0.77, 0.62, 0.77, 0.73, 0.83, 0.70, and 0.71 respectively via Support Vector Machine. Further, all the QSAR models performed equally well on independent validation data sets. Additionally, we also checked the performance of the random forest machine learning technique for the above datasets. The integrated analysis tools can convert the chemical structure into different formats, search for a similar chemical in the aBiofilm database and design the analogs. Moreover, the data visualization modules check the distribution of experimentally validated biofilm inhibitors according to their common scaffolds. The Biofilm-i platform would be of immense help to researchers engaged in designing highly efficacious biofilm inhibitors for tackling the menace of antibiotic drug resistance

A Novel Modification of Semilunar Coronally Advanced Flap for the Treatment of Gingival Recession: A Case Report

Recession of the gingival margin is still a common problem, with negative consequences for both aesthetics and periodontal health. Complete root coverage of gingival recession defects with periodontal plastic surgery is a necessity in this era where aesthetics are a top priority. Correction of mucogingival recession deformities has been described using a variety of periodontal plastic surgical procedures, each with varying degrees of success. The goal of this case report is to describe how a semilunar coronally advanced flap was modified to treat gingival recession in the maxillary anterior tooth