Radar HRRP Modeling using Dynamic System for Radar Target Recognition

High resolution range profile (HRRP) is being known as one of the most powerful tools for radar target recognition. The main problem with range profile for radar target recognition is its sensitivity to aspect angle. To overcome this problem, consecutive samples of HRRP were assumed to be identically independently distributed (IID) in small frames of aspect angles in most of the related works. Here, considering the physical circumstances of maneuver of an aerial target, we have proposed dynamic system which models the short dependency between consecutive samples of HRRP in segments of the whole HRRP sequence. Dynamic system (DS) is used to model the sequence of PCA (principal component analysis) coefficients extracted from the sequence of HRRPs. Considering this we have proposed a model called PCA+DS. We have also proposed a segmentation algorithm which segments the HRRP sequence reliably. Akaike information criterion (AIC) used to evaluate the quality of data modeling showed that our PCA+DS model outperforms factor analysis (FA) model. In addition, target recognition results using simulated data showed that our method based on PCA+DS achieves better recognition rates compared to the method based on FA

The emerging role of exosomal miRNAs as a diagnostic and therapeutic biomarker in Mycobacterium tuberculosis infection

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), has been the world�s driving fatal bacterial contagious disease globally. It continues a public health emergency, and around one-third of the global community has been affected by latent TB infection (LTBI). This is mostly due to the difficulty in diagnosing and treating patients with TB and LTBI. Exosomes are nanovesicles (40�100 nm) released from different cell types, containing proteins, lipids, mRNA, and miRNA, and they allow the transfer of one�s cargo to other cells. The functional and diagnostic potential of exosomal miRNAs has been demonstrated in bacterial infections, including TB. Besides, it has been recognized that cells infected by intracellular pathogens such as Mtb can be secreting an exosome, which is implicated in the infection�s fate. Exosomes, therefore, open a unique viewpoint on the investigative process of TB pathogenicity. This study explores the possible function of exosomal miRNAs as a diagnostic biomarker. Moreover, we include the latest data on the pathogenic and therapeutic role of exosomal miRNAs in TB. © 2021, The Author(s)

Radar HRRP Modeling using Dynamic System for Radar Target Recognition

High resolution range profile (HRRP) is being known as one of the most powerful tools for radar target recognition. The main problem with range profile for radar target recognition is its sensitivity to aspect angle. To overcome this problem, consecutive samples of HRRP were assumed to be identically independently distributed (IID) in small frames of aspect angles in most of the related works. Here, considering the physical circumstances of maneuver of an aerial target, we have proposed dynamic system which models the short dependency between consecutive samples of HRRP in segments of the whole HRRP sequence. Dynamic system (DS) is used to model the sequence of PCA (principal component analysis) coefficients extracted from the sequence of HRRPs. Considering this we have proposed a model called PCA+DS. We have also proposed a segmentation algorithm which segments the HRRP sequence reliably. Akaike information criterion (AIC) used to evaluate the quality of data modeling showed that our PCA+DS model outperforms factor analysis (FA) model. In addition, target recognition results using simulated data showed that our method based on PCA+DS achieves better recognition rates compared to the method based on FA

Immunization of mice by the co-administration of codon-optimized HPV16 E7 and lL12 genes against HPV16-associated cervical cancer

Background: Various promising procedures have been used to improve the potency of DNA vaccines for the treatment of human papillomavirus type 16 (HPV16) infections. Interleukin-12 (IL12) is a powerful adjuvant that can contribute to T cell-mediated protection against many pathogens, specifically viruses. Considering the important role of T cell-mediated immunity in tumor clearance, the induction of these responses can help control the progression of tumors in animal models. We have demonstrated that the co-administration of codon-optimized E7 (uE7) gene of HPV16 with interleukin-12 is effective in the development of antitumor responses. Objectives: The present study examined the co-administration of codon-optimized HPV16 E7 gene with murine interleukin-12 gene (mIL-12) as a vaccine adjuvant in tumor mice model. Materials and methods: C57BL/6 mice were studied for tumor progression after injection of recombinant DNA vaccines. Lactate dehydrogenase (LDH) and IFN-γ were measured to evaluate the activity of cytotoxic T lymphocytes (CTLs). Measurements of tumor volume and MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay were used for assessment of therapeutic antitumor effects of the vaccines. Results: Results showed that DNA vaccines, specifically codon-optimized E7/murine interleukin-12 (mIL-12), elicited significant differences in levels of IFN-γ and cytotoxic T lymphocyte (CTLs) responses compared to control groups. Furthermore, higher antitumor response and lower tumor size in the vaccine group was significantly evident compared to control group. Conclusion: The co-administration of codon-optimized HPV16 E7 gene with IL12 significantly enhances the DNA vaccine potency against HPV16-associated cervical cancer. © 2019 Elsevier Lt

E6-specific detection and typing of human papillomaviruses in oral cavity specimens from Iranian patients

Background: Detection and quantification of human Papillomavirus (HPV) genome in oral carcinoma play an important role in diagnosis, as well as implications for progression of disease. Methods: We evaluated tissues from 50 esopharyngeal cancers collected from different regions of Iran for HPV E6 using the two type-specific primers sets. E6 gene of HPV genotypes was amplified by specific primers. The sensitivity of PCR assay was analyzed and determined using HPV-DNA-containing plasmids. Real-time PCR was utilized to determine the prevalence and HPV viral load in patients with oral cavity squamous cell carcinoma. Results: Eighteen (36) specimens were positive for HPV. Among the 18 positive specimens, 10 showed HPV-18 (55.55), and 8 specimens were positive for HPV-11 (44.44). Of the 18 infected specimens, 6 (33.32) and 12 (66.65) were identified as high-titer and low-titer viral load, respectively. Conclusions: The PCR-based assay, developed in the current study, could be used for HPV detection, quantification, and genotyping in epidemiological and clinical studies. © 2017, Pasteur Institute of Iran. All rights reserved

Antiviral therapeutic potential of curcumin: An update

The treatment of viral disease has become a medical challenge because of the increasing incidence and prevalence of human viral pathogens, as well as the lack of viable treatment alternatives, including plant-derived strategies. This review attempts to investigate the trends of research on in vitro antiviral effects of curcumin against different classes of human viral pathogens worldwide. Various electronic databases, including PubMed, Scopus, Web of Science, and Google Scholar were searched for published English articles evaluating the anti-viral activity of curcumin. Data were then extracted and analyzed. The forty-three studies (published from 1993 to 2020) that were identified contain data for 24 different viruses. The 50 cytotoxic concentration (CC50), 50 effective/inhibitory concentration (EC50/IC50), and stimulation index (SI) parameters showed that curcumin had antiviral activity against viruses causing diseases in humans. Data presented in this review highlight the potential antiviral applications of curcumin and open new avenues for further experiments on the clinical applications of curcumin and its derivatives. © 2021 by the authors. Licensee MDPI, Basel, Switzerland

Genotyping and sequence characterization of the NSP4 gene of human group A rotavirus strains in Northern Iran

Rotavirus is known to be responsible for remarkable numbers of severe diarrheal episodes and even death in infants and young children. In this study, we aimed to survey genetic diversity and variation analysis of viroporin, which is encoded by the rotavirus NSP4 segment. Thirty-five rotavirus-positive specimens were obtained, and RNA extraction and polymerase chain reaction amplification were performed. After the sequencing process, four specimens were excluded, and the final 31 samples remained for genetic diversity and variation analysis. The predominant single G/P combination was G1P8 (~78%), followed by G2P8 (~13%), and equal percentages (3%) of G2P4, G3P8, and G-non-typeable-P8. Further analyses revealed that variations could be found in the three regions of NSP4, including VP4 binding site (aa 112�146), double-layered particle binding site (aa 161�175), and finally, in the predicted amphipathic alpha-helix. Phylogenic tree analysis demonstrated that the mentioned samples clustered with genotype E1 and E2 reference sequences. As previously reported in the literature, in this study, it was revealed that no apparent correlation exists in the deduced amino acid sequences corresponding to this region between the rotaviruses collected from patients with and without diarrhea. © 2021 Wiley Periodicals LL

Hepatitis C virus alternative reading frame protein (ARFP): Production, features, and pathogenesis

Earlier observation suggests that hepatitis C virus (HCV) is a single-stranded RNA virus which encodes at least 10 viral proteins. F protein is a novel protein which has been discovered recently. These studies suggest three mechanisms for the production of this protein concerning ribosomal frameshift at codon 10, initial translation at codons 26 and 85 or 87. In this study, the association between protein F and chronicity of hepatocellular carcinoma (HCC) has been reviewed. Evidence suggests that humoral immune system can recognize this protein and produce antibodies against it. By detecting antibodies in infected people, investigators found that F protein might have a role in HCV infection causing chronic cirrhosis and HCC as higher prevalence was found in patients with mentioned complications. The increment of CD4+, CD25+, and FoxP3+ T cells, along with CD8+ T cells with low expression of granzyme B, also leads to weaker responses of the immune system which helps the infection to become chronic. Moreover, it contributes to the survival of the virus in the body through affecting the production of interferon. F protein also might play roles in the disease development, resulting in HCC. The existence of F protein affects cellular pathways through upregulating p53, c-myc, cyclin D1, and phosphorylating Rb. This review will summarize these effects on immune system and related mechanisms in cellular pathways. © 2020 Wiley Periodicals LL