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E6-specific detection and typing of human papillomaviruses in oral cavity specimens from Iranian patients
Authors
M. Ajorloo
A. Ardebili
+6 more
S. Khanizadeh
H.R. Nikoo
M.H. Pouriayevali
M. Ravanshad
F. Rezaei
A. Teimoori
Publication date
1 January 2017
Publisher
Pasteur Institute of Iran
Abstract
Background: Detection and quantification of human Papillomavirus (HPV) genome in oral carcinoma play an important role in diagnosis, as well as implications for progression of disease. Methods: We evaluated tissues from 50 esopharyngeal cancers collected from different regions of Iran for HPV E6 using the two type-specific primers sets. E6 gene of HPV genotypes was amplified by specific primers. The sensitivity of PCR assay was analyzed and determined using HPV-DNA-containing plasmids. Real-time PCR was utilized to determine the prevalence and HPV viral load in patients with oral cavity squamous cell carcinoma. Results: Eighteen (36) specimens were positive for HPV. Among the 18 positive specimens, 10 showed HPV-18 (55.55), and 8 specimens were positive for HPV-11 (44.44). Of the 18 infected specimens, 6 (33.32) and 12 (66.65) were identified as high-titer and low-titer viral load, respectively. Conclusions: The PCR-based assay, developed in the current study, could be used for HPV detection, quantification, and genotyping in epidemiological and clinical studies. © 2017, Pasteur Institute of Iran. All rights reserved
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Last time updated on 19/03/2018