Subsurface Geophysical Investigation and Physiochemical/Microbial Analysis of Groundwater Contaminant in Ota, Southwestern Nigeria

Geoelectrical imaging of the subsurface to investigate groundwater contaminants in a sedimentary environment like Ota, South-western Nigeria was carried out. The 2 – Dimensional electrical resistivity survey method for the subsurface imaging was done by engaging the Wenner array configuration using PASI Resistivity Meter in the surface measurement. The vertical electrical sounding (VES) was also done using the Schlumberger array configuration to determine the depth to the aquifer layer. The DIPRO software and the WIN-Resist application software were used for the interpretation of the 2 – D resistivity data and VES data respectively. Both Hydrochemical and Microbial analyses were done to test for the presence of heavy metals and the estimation of Total Aerobic Bacteria (TAB). From the VES results the depth to the aquifer lies around 20.1m (60.33ft.) of resistivity of 34.1 Ώ-m and the 2–D results showed the resistivities as 28.0 Ώ-m and 47.0 Ώ-m respectively showing that the study area has groundwater potential. For the hydrochemical analysis on heavy metals, the result lies within World Health Organization (WHO) standard for drinking water. But for the microbial analysis, total aerobic plate count of 2.3×106 for drinking water is high and above WHO standard specification for drinking wate

Clathrin regulates lymphocyte migration by driving actin accumulation at the cellular leading edge

Lymphocyte migration, which is essential for effective immune responses, belongs to the so-called amoeboid migration. The lymphocyte migration is up to 100 times faster than between mesenchymal and epithelial cell types. Migrating lymphocytes are highly polarized in three well-defined structural and functional zones: uropod, medial zone, and leading edge (LE). The actiomyosin-dependent driving force moves forward the uropod, whereas massive actin rearrangements protruding the cell membrane are observed at the LE. These actin rearrangements resemble those observed at the immunological synapse driven by clathrin, a protein normally involved in endocytic processes. Here, we used cell lines as well as primary lymphocytes to demonstrate that clathrin and clathrin adaptors colocalize with actin at the LE of migrating lymphocytes, but not in other cellular zones that accumulate both clathrin and actin. Moreover, clathrin and clathrin adaptors, including Hrs, the clathrin adaptor for multivesicular bodies, drive local actin accumulation at the LE. Clathrin recruitment at the LE resulted necessary for a complete cell polarization and further lymphocyte migration in both 2D and 3D migration models. Therefore, clathrin, including the clathrin population associated to internal vesicles, controls lymphocyte migration by regulating actin rearrangements occurring at the LE.This work was supported by the grants from the Spanish Ministry of Science and Technology (MICINN; BFU2011-29450 to E.V.) and Ministry of Economy and Competitiveness (MINECO; SAF2014-56716-REDT and BFU2014-59585-R to E.V., SAF2011-25834 to F.S.M., SAF2014-58895-JIN to A.C.A and BFU2014-54181-P to J.L.C.), the Madrid regional government (INDISNET-S2011/BMD-2332 to F.S.M.) and the European Research Council (ERC-2011-AdG 294340-GENTRIS to F.S.M.). We are grateful to the “Centro de Transfusión” of the “Comunidad Autónoma de Madrid” for providing the Buffy Coats

Orphan CpG islands define a novel class of highly active enhancers

<p>CpG islands (CGI) are critical genomic regulatory elements that support transcriptional initiation and are associated with the promoters of most human genes. CGI are distinguished from the bulk genome by their high CpG density, lack of DNA methylation, and euchromatic features. While CGI are canonically known as strong promoters, thousands of ‘orphan’ CGI lie far from any known transcript, leaving their function an open question. We undertook a comprehensive analysis of the epigenetic state of orphan CGI across over 100 cell types. Here we show that most orphan CGI display the chromatin features of active enhancers (H3K4me1, H3K27Ac) in at least one cell type. Relative to classical enhancers, these enhancer CGI (ECGI) are stronger, as gauged by chromatin state and in functional assays, are more broadly expressed, and are more highly conserved. Likewise, ECGI engage in more genomic contacts and are enriched for transcription factor binding relative to classical enhancers. In human cancers, these epigenetic differences between ECGI vs. classical enhancers manifest in distinct alterations in DNA methylation. Thus, ECGI define a class of highly active enhancers, strengthened by the broad transcriptional activity, CpG density, hypomethylation, and chromatin features they share with promoter CGI. In addition to indicating a role for thousands of orphan CGI, these findings suggests that enhancer activity may be an intrinsic function of CGI in general and provides new insights into the evolution of enhancers and their epigenetic regulation during development and tumorigenesis.</p

Clinical management of a complicated crown-root fracture using autogenous tooth fragment: A biological restorative approach

Trauma resulting in crown-root fracture is one of the most challenging fracture types. However, biologic width involvement should be carefully evaluated. Reattachment of tooth fragment to a fractured tooth remains as the treatment of choice because of its simplicity, natural esthetics, and conservation of tooth structure. The reattachment procedure using composite resin should be considered if the subgingival fracture can be exposed to provide isolation. This report presents a case of complicated crown-root fracture of permanent maxillay left central incisor, involving the biologic width in a 10-year-old girl. The traumatized tooth was treated endodontically. Access to the subgingival margins was gained by orthodontic extrusion followed by gingivectomy. The fractured fragment was reattached using bonding system and composite resin