Glucosyl anthranilate

In the crystal structure of the title compound, C21H25NO11, the hexopyranosyl ring adopts a chair conformation and the five substituents are in equatorial positions. An intra­molecular hydrogen bond between the amino group and a neighbouring carbonyl group is found. Two carbonyl groups are disordered and were refined using a split model

Human-derived IgG level as an indicator for EBV-associated lymphoma model in Hu-PBL/SCID chimeras

<p>Abstract</p> <p>Background</p> <p>Epstein-Barr virus (EBV) has a close association with various types of human lymphomas. Animal models are essential to elucidate the pathogenesis of human EBV-associated lymphomas. The aim of the present study is to evaluate the association between human IgG concentration and EBV-associated lymphoma development in huPBL/SCID mice.</p> <p>Methods</p> <p>Human peripheral blood lymphocytes (hu-PBL) from EBV-seropositive donors were inoculated intraperitoneally into SCID mouse. Immunohistochemical staining was used to examine differentiated antigens of tumor cells. EBV infection of the induced tumors was detected by <it>in situ </it>hybridization. IgG concentrations in the serums of 12 SCID mice were measured by unidirectional immunodiffusion assay.</p> <p>Results</p> <p>21 out of 29 mice developed tumors in their body. Immunohistochemical staining showed that all induced tumors were LCA (leukocyte common antigen) positive, B-cell markers (CD20, CD79a) positive, and T-cell markers (both CD3 and CD45RO) negative. The tumors can be diagnosed as human B-cell lymphomas by these morphological and immunohistochemical features. In situ hybridization exhibited resultant tumor cells had EBV encoded small RNA-1 (EBER-1). Human-derived IgG could be found in the serum from SCID mice on the 15^thday following hu-PBL transplantation, and IgG levels increased with the tumor development in 6 hu-PBL/SCID chimeras.</p> <p>Conclusions</p> <p>Intraperitoneal transfer of hu-PBLs from EBV+ donors to SCID mice leads to high human IgG levels in mouse serum and B cell lymphomas. Our findings suggest that increasing levels of human-derived IgG in peripheral blood from hu-PBL/SCID mice could be used to monitor EBV-related human B-cell lymphoma development in experimental animals.</p

An Intensified Mode of Variability Modulating the Summer Heat Waves in Eastern Europe and Northern China

This study investigates the leading pattern of Eurasian summer heat waves (HWs) using observed and simulated data sets and reveals an intensified mode of variability that bridges the HWs in eastern Europe (EE) and northern China (NC). The concurrent variability of the HWs in EE and NC is primarily driven by an atmospheric circum-global teleconnection that induces anomalous anticyclones over the two regions. The observed upward trends in EE and NC HW days could be related to the warm sea surface temperatures around Greenland Island, which may weaken the Atlantic westerly jet stream and lead to amplified wave trains at the exit of the jet, resulting in strengthened anticyclones over EE and NC that favor the occurrences of HWs. The Geophysical Fluid Dynamics Laboratory high-resolution atmospheric model fails to simulate the EE and NC HWs, due probably to the model’s poor representation of the South Asian summer rainfall

Transgenic studies reveal the positive role of LeEIL-1 in regulating shikonin biosynthesis in Lithospermum erythrorhizon hairy roots

Time-course accumulation of shikonin in four typical hairy root lines. Value of Ei-19 or EO-13 is significantly different from that of the control line WT-1 or EV-9 at each time point from 3 to 12 days, respectively (Student’s t-test, P < 0.05). (TIF 125 kb

The longitudinal association between possible new sarcopenia and the depression trajectory of individuals and their intimate partners

BackgroundIt is currently unknown whether the dynamic nature of depression affects the development of sarcopenia. Herein, this study aims to assess the association between possible new sarcopenia and the depression trajectory of individuals and their intimate partners through a 4-year longitudinal cohort study.MethodsOur study included 784 pairs of individuals without possible sarcopenia and their spouses from the China Health and Retirement Longitudinal Study (CHARLS) 2011. All individuals and their spouses received three assessments of the Center for Epidemiologic Studies Depression 10-item (CESD-10) scale in 2011, 2013, and 2015. According to the diagnostic algorithm recommended by the Asian Working Group for Sarcopenia (AWGS) 2019, we evaluated the incidence of possible sarcopenia in individuals in 2015. Latent class analysis (LCA) was used to identify a longitudinal depression trajectory of individuals and their spouses during a 4-year follow-up. Subsequently, we assessed the relationship between possible sarcopenia and depression trajectory using three generalized additive models.ResultsIn 2015, 24.87% (195/784) of individuals were diagnosed with possible sarcopenia. LCA identified five depression trajectories: a persistently high risk of depression in individuals and their spouses (reference; class 1 = 34 [4.3%]); a persistently low risk of depression in individuals and their spouses (class 2 = 526 [67.1%]); a high risk of depression in individuals and a low risk of depression in spouses (class 3 = 46 [5.9%]); a low risk of depression in individuals and a high risk of depression in spouses (class 4 = 116 [14.8%]); and a reduced risk of depression in individuals and their spouses (class 5 = 62 [7.9%]). The highest incidence of possible sarcopenia was shown in class 1, followed by classes 3 and 5. Classes 2 (adjusted relative risk (RR) = 0.44, 95% confidence interval (CI): 0.20–0.97) and 4 (adjusted RR = 0.40, 95%CI: 0.17–0.96) had a significantly lower incidence of possible sarcopenia than class 1. Subgroup analysis demonstrated that the incidence of possible sarcopenia in class 4 was obviously higher in women (38.89%) than in men (18.4%).ConclusionsOur study indicates a persistently high risk of depression in individuals to develop possible sarcopenia. In addition, a persistently high risk of depression in intimate partners potentially increases the risk of possible new sarcopenia, especially in female individuals who are at low risk of depression

Study on the Moderating Effect of Body Mass Index in Correlation of Anxiety and Depression Disorders

BackgroundAs two different kinds of mental disorders, anxiety disorder and depressive disorder could probably coexist in one with the proceeding of the illness. However, there are few studies on how to prevent and treat the coexistence of anxiety and depressive disorders.ObjectiveTo discuss the relationship between anxiety and depressive disorders, and the moderating effect of body mass index (BMI) in their relationship.MethodsBy use of simple random sampling, 86 outpatients and inpatients with anxiety disorders were selected from the First Affiliated Hospital of Nanchang University during June 1st to August, 31st, 2021. A self-made demographic questionnaire was used to obtain the demographic information. The Hamilton Anxiety Rating Scale was used to assess the anxiety level. The Hamilton Rating Scale for Depression was used to assess the depression level. Pearson correlation analyses were performed to assess the relationship between anxiety and depression disorders, and that between BMI and anxiety or depression disorder. Hierarchical regression analysis was adopted to explore the moderating effect of BMI on the relationship between anxiety and depressive disorders.ResultsEighty eligible cases were also diagnosed with depression. The anxiety prevalence was significantly increased with depression prevalence (r=0.70, P&lt;0.01) . BMI had no significant linear correlation with anxiety prevalence (r=0.03, P&gt;0.05) . BMI also had no significant linear correlation with depression prevalence (r=0.14, P&gt;0.05) . BMI moderated the relationship between anxiety and depression disorders significantly (&#x03B2;=-0.16, P&lt;0.01) .ConclusionBMI can weaken the effect of anxiety disorder on depression, helping prevent them from developing into comorbid mental disorders, providing new ideas for developing new dietary standard or exercise patterns for mental health prevention and treatment in the future and expands the research field of nutritional psychiatry to a certain extent

Ultra-Low-Frequency Radio Astronomy Observations from a Selenocentric Orbit: first results of the Longjiang-2 experiment

This paper introduces the first results of observations with the Ultra-Long-Wavelength (ULW) -- Low Frequency Interferometer and Spectrometer (LFIS) on board the selenocentric satellite Longjiang-2. We present a brief description of the satellite and focus on the LFIS payload. The in-orbit commissioning confirmed a reliable operational status of the instrumentation. We also present results of a transition observation, which offers unique measurements on several novel aspects. We estimate the RFI suppression required for such a radio astronomy instrumentation at the Moon distances from Earth to be of the order of 80 dB. We analyse a method of separating Earth- and satellite-originated radio frequency interference (RFI). It is found that the RFI level at frequencies lower than a few MHz is smaller than the receiver noise floor.Comment: Accepted for publication in Experimental Astronomy; 22 pages, 11 figure

Spread of Streptococcus suis Sequence Type 7, China

Streptococcus suis sequence type (ST) 7 has been spreading throughout China. To determine events associated with its emergence, we tested 114 isolates. In all 106 ST7 strains responsible for human outbreaks and sporadic infections, the tetracycline-resistance gene, tetM, was detected on the conjugative transposon Tn916. Horizontal transmission of tetM is suspected

Multiple ITS Copies Reveal Extensive Hybridization within Rheum (Polygonaceae), a Genus That Has Undergone Rapid Radiation

During adaptive radiation events, characters can arise multiple times due to parallel evolution, but transfer of traits through hybridization provides an alternative explanation for the same character appearing in apparently non-sister lineages. The signature of hybridization can be detected in incongruence between phylogenies derived from different markers, or from the presence of two divergent versions of a nuclear marker such as ITS within one individual.In this study, we cloned and sequenced ITS regions for 30 species of the genus Rheum, and compared them with a cpDNA phylogeny. Seven species contained two divergent copies of ITS that resolved in different clades from one another in each case, indicating hybridization events too recent for concerted evolution to have homogenised the ITS sequences. Hybridization was also indicated in at least two further species via incongruence in their position between ITS and cpDNA phylogenies. None of the ITS sequences present in these nine species matched those detected in any other species, which provides tentative evidence against recent introgression as an explanation. Rheum globulosum, previously indicated by cpDNA to represent an independent origin of decumbent habit, is indicated by ITS to be part of clade of decumbent species, which acquired cpDNA of another clade via hybridization. However decumbent and glasshouse morphology are confirmed to have arisen three and two times, respectively.These findings suggested that hybridization among QTP species of Rheum has been extensive, and that a role of hybridization in diversification of Rheum requires investigation

SH3 interactome conserves general function over specific form

Src homology 3 (SH3) domains bind peptides to mediate protein–protein interactions that assemble and regulate dynamic biological processes. We surveyed the repertoire of SH3 binding specificity using peptide phage display in a metazoan, the worm Caenorhabditis elegans, and discovered that it structurally mirrors that of the budding yeast Saccharomyces cerevisiae. We then mapped the worm SH3 interactome using stringent yeast two-hybrid and compared it with the equivalent map for yeast. We found that the worm SH3 interactome resembles the analogous yeast network because it is significantly enriched for proteins with roles in endocytosis. Nevertheless, orthologous SH3 domain-mediated interactions are highly rewired. Our results suggest a model of network evolution where general function of the SH3 domain network is conserved over its specific form