Effect of Toki-Shakuyaku-San on Regional Cerebral Blood Flow in Patients with Mild Cognitive Impairment and Alzheimer's Disease

The aim of this study was to examine the effect of toki-shakuyaku-san (TSS) on mild cognitive impairment (MCI) and Alzheimer's disease (AD) using single-photon emission computed tomography (SPECT). All subjects were administered TSS (7.5 g/day) for eight weeks. SPECT and evaluations using the Mini Mental State Examination (MMSE), Neuropsychiatric Inventory, and Physical Self-Maintenance Scale were performed before and after treatment with TSS. Three patients with MCI and five patients with AD completed the study. No adverse events occurred during the study period. After treatment with TSS, regional cerebral blood flow (rCBF) in the posterior cingulate was significantly higher than that before treatment. No brain region showed a significant decrease in rCBF. TSS treatment also tended to improve the score for orientation to place on the MMSE. These results suggest that TSS could be useful for treatment of MCI and AD

Effect of Toki-Shakuyaku-San on Regional Cerebral Blood Flow in Patients with Mild Cognitive Impairment and Alzheimer's Disease

The aim of this study was to examine the effect of toki-shakuyaku-san (TSS) on mild cognitive impairment (MCI) and Alzheimer's disease (AD) using single-photon emission computed tomography (SPECT). All subjects were administered TSS (7.5 g/day) for eight weeks. SPECT and evaluations using the Mini Mental State Examination (MMSE), Neuropsychiatric Inventory, and Physical Self-Maintenance Scale were performed before and after treatment with TSS. Three patients with MCI and five patients with AD completed the study. No adverse events occurred during the study period. After treatment with TSS, regional cerebral blood flow (rCBF) in the posterior cingulate was significantly higher than that before treatment. No brain region showed a significant decrease in rCBF. TSS treatment also tended to improve the score for orientation to place on the MMSE. These results suggest that TSS could be useful for treatment of MCI and AD

Protein kinase C-δ signaling regulates glucagon secretion from pancreatic islets

Accumulating evidence supports the “glucagonocentric hypothesis”, in which antecedent α-cell failure and inhibition of glucagon secretion are responsible for diabetes progression. Protein kinase C (PKC) is involved in glucagon secretion from α-cells, although which PKC isozyme is involved and the mechanism underlying this PKC-regulated glucagon secretion remains unknown. Here, the involvement of PKCδ in the onset and progression of diabetes was elucidated. Immunofluorescence studies revealed that PKCδ was expressed and activated in α-cells of STZ-induced diabetic model mice. Phorbol 12-myristate 13-acetate (PMA) stimulation significantly augmented glucagon secretion from isolated islets. Pre-treatment with quercetin and rottlerin, PKCδ signaling inhibitors, significantly suppressed the PMA-induced elevation of glucagon secretion. While Go6976, a Ca2+ - dependent PKC selective inhibitor did not suppress glucagon secretion. Quercetin suppressed PMA-induced phosphorylation of Tyr311 of PKCδ in isolated islets. However, quercetin itself had no effect on either glucagon secretion or glucagon mRNA expression. Our data suggest that PKCδ signaling inhibitors suppressed glucagon secretion. Elucidation of detailed signaling pathways causing PKCδ activation in the onset and progression of diabetes followed by the augmentation of glucagon secretion could lead to the identification of novel therapeutic target molecules and the development of novel therapeutic drugs for diabetes

Christopher Simpson The Division-Viol, or, The Art of PLAYING Extempore upon a GROUND. EDITIO SECVNDA Dedication & Recommendation

本訳稿はChristopher Simpson (1605頃－1669) 著 The Division-Viol, or, The Art of PLAYING Ex tempore upon a GROUND. DIVIDED INTO THREE PARTS. EDITIO SECVNDA, London, 1665 の著者による献辞および楽譜出版権所有者による推薦文の全訳である

Study of the valenčního belt of transition metals and their alloys

Nazev prace: Studium valencniho pdsu prechodovych kovu ajejich slitin Autor: Michal Skoda Katedra (ustav): Katedra elektroniky a vakuove fyziky Vedouci diplomove prace: Prof. RNDr. Vladimir Matolin, DrSc. E-mail vedouciho: [email protected] Abstrakt: Prace se zabyvd studiem bimetalickeho systemu Ce/Pd(lll) metodami fotoelektronove spektroskopie (PES). Vysledky ziskane metodou rezonancni fotoelektronove spektroskopie (RPES) ukazuji, ze zmena chemickeho stavu systemu je zfejme doprovdzena vyznamnou modifikaci pdsove struktury atomu sytemu, kterd se projevuje dramatickymi zmenami obzvldste ve valencnim pdsu. Metoda RPES se prokdzala byt velmi mocnym ndslrojem ke studiu elekironove struktury valencniho pdsu. Klicova slova:^P5, SRPES, RPES, Pd, Ce Title: Valence band study of transition metals and their alloys Author: Michal Skoda Department: Department of Electronics and Vacuum Physics Supervisor: Prof. RNDr. Vladimir Matolin, DrSc. Supervisor's e-mail address: [email protected] Abstract: The work deals with study of bimetallic system Ce/Pd(lll) by photoelectron spectroscopy method (PES). Its investigation by resonant photoelectron spectroscopy (RPES) showed, that the chemical state modifications of the system induce significant modifications in the band structure of its atoms...Nazev prace: Studium valencniho pdsu prechodovych kovu ajejich slitin Autor: Michal Skoda Katedra (ustav): Katedra elektroniky a vakuove fyziky Vedouci diplomove prace: Prof. RNDr. Vladimir Matolin, DrSc. E-mail vedouciho: [email protected] Abstrakt: Prace se zabyvd studiem bimetalickeho systemu Ce/Pd(lll) metodami fotoelektronove spektroskopie (PES). Vysledky ziskane metodou rezonancni fotoelektronove spektroskopie (RPES) ukazuji, ze zmena chemickeho stavu systemu je zfejme doprovdzena vyznamnou modifikaci pdsove struktury atomu sytemu, kterd se projevuje dramatickymi zmenami obzvldste ve valencnim pdsu. Metoda RPES se prokdzala byt velmi mocnym ndslrojem ke studiu elekironove struktury valencniho pdsu. Klicova slova:^P5, SRPES, RPES, Pd, Ce Title: Valence band study of transition metals and their alloys Author: Michal Skoda Department: Department of Electronics and Vacuum Physics Supervisor: Prof. RNDr. Vladimir Matolin, DrSc. Supervisor's e-mail address: [email protected] Abstract: The work deals with study of bimetallic system Ce/Pd(lll) by photoelectron spectroscopy method (PES). Its investigation by resonant photoelectron spectroscopy (RPES) showed, that the chemical state modifications of the system induce significant modifications in the band structure of its atoms...Katedra fyziky povrchů a plazmatuDepartment of Surface and Plasma ScienceFaculty of Mathematics and PhysicsMatematicko-fyzikální fakult

Development of a quantitative pachytene chromosome map and its unification with somatic chromosome and linkage maps of rice (<i>Oryza sativa</i> L.)

<div><p>A quantitative pachytene chromosome map of rice (<i>Oryza sativa</i> L.) was developed using imaging methods. The map depicts not only distribution patterns of chromomeres specific to pachytene chromosomes, but also the higher order information of chromosomal structures, such as heterochromatin (condensed regions), euchromatin (decondensed regions), the primary constrictions (centromeres), and the secondary constriction (nucleolar organizing regions, NOR). These features were image analyzed and quantitatively mapped onto the map by <u>Ch</u>romosome <u>I</u>mage <u>A</u>nalyzing <u>S</u>ystem ver. 4.0 (CHIAS IV). Correlation between H3K9me2, an epigenetic marker and formation and/or maintenance of heterochromatin, thus was, clearly visualized. Then the pachytene chromosome map was unified with the existing somatic chromosome and linkage maps by physically mapping common DNA markers among them, such as a rice A genome specific tandem repeat sequence (TrsA), 5S and 45S ribosomal RNA genes, five bacterial artificial chromosome (BAC) clones, four P1 bacteriophage artificial chromosome (PAC) clones using multicolor fluorescence <i>in situ</i> hybridization (FISH). Detailed comparison between the locations of the DNA probes on the pachytene chromosomes using multicolor FISH, and the linkage map enabled determination of the chromosome number and short/long arms of individual pachytene chromosomes using the chromosome number and arm assignment designated for the linkage map. As a result, the quantitative pachytene chromosome map was unified with two other major rice chromosome maps representing somatic prometaphase chromosomes and genetic linkages. In conclusion, the unification of the three rice maps serves as an indispensable basic information, not only for an in-depth comparison between genetic and chromosomal data, but also for practical breeding programs.</p></div