γ-ray spectroscopy of C19 via the single-neutron knock-out reaction

The one-neutron knock-out reaction H-1(C-20, C-19 gamma) was studied at RIKEN using the DALI2 array. A gamma-ray transition was observed at 198(10) keV. Based on the comparison between the experimental production cross section and theoretical predictions, the transition was assigned to the de-excitation of the 3/2(1)(+) state to the ground state

Nuclear structure study of 19,20,21N nuclei by gamma spectroscopy

The structure of neutron rich nitrogen nuclei has been studied by use of neutron removal reaction and inelastic scattering. Mass and charge deformations have been deduced for the first excited state of 21N, which indicates the partial persitence of the N=14 subshell closure in nitrogen isotopes. The spectroscopic information obtained on the structure of 19,20,21N confirms the results from a previous experiment

Self-Consistent Velocity Dependent Effective Interactions

The theory of self-consistent effective interactions in nuclei is extended for a system with a velocity dependent mean potential. By means of the field coupling method, we present a general prescription to derive effective interactions which are consistent with the mean potential. For a deformed system with the conventional pairing field, the velocity dependent effective interactions are derived as the multipole pairing interactions in doubly-stretched coordinates. They are applied to the microscopic analysis of the giant dipole resonances (GDR's) of ${}^{148,154}Sm$, the first excited $2^+$ states of Sn isotopes and the first excited $3^-$ states of Mo isotopes. It is clarified that the interactions play crucial roles in describing the splitting and structure of GDR peaks, in restoring the energy weighted sum rule, and in reducing the values of $B(E\lambda)$.Comment: 35 pages, RevTeX, 7 figures (available upon request), to appear in Phys.Rev.

An international validation study of the IL-2 Luc assay for evaluating the potential immunotoxic effects of chemicals on T cells and a proposal for reference data for immunotoxic chemicals

To evaluate the immunotoxic effects of xenobiotics, we have established the Multi-ImmunoTox assay, in which three stable reporter cell lines are used to evaluate the effects of chemicals on the IL-2, IFN-\u3b3, IL-1\u3b2 and IL-8 promoters. Here, we report the official validation study of the IL-2 luciferase assay (IL-2 Luc assay). In the Phase I study that evaluated five coded chemicals in three sets of experiments, the average within-laboratory reproducibility was 86.7%. In the Phase II study, 20 coded chemicals were evaluated at multiple laboratories. In the combined results of the Phase I and II studies, the between-laboratory reproducibility was 80.0%. These results suggested that the IL-2 Luc assay was reproducible both between and within laboratories. To determine the predictivity, we collected immunotoxicological information and constructed the reference data by classifying the chemical into immunotoxic compounds targeting T cells or others according to previously reported criteria. When compared with the reference data, the average predictivity of the Phase I and II studies was 75.0%, while that of additional 60 chemicals examined by the lead laboratory was 82.5%. Although the IL-2 Luc assay alone is not sufficient to predict immunotoxicity, it will be a useful tool when combined with other immune tests

Novel calmodulin mutations associated with congenital arrhythmia susceptibility.

BACKGROUND: Genetic predisposition to life-threatening cardiac arrhythmias such as congenital long-QT syndrome (LQTS) and catecholaminergic polymorphic ventricular tachycardia (CPVT) represent treatable causes of sudden cardiac death in young adults and children. Recently, mutations in calmodulin (CALM1, CALM2) have been associated with severe forms of LQTS and CPVT, with life-threatening arrhythmias occurring very early in life. Additional mutation-positive cases are needed to discern genotype-phenotype correlations associated with calmodulin mutations. METHODS AND RESULTS: We used conventional and next-generation sequencing approaches, including exome analysis, in genotype-negative LQTS probands. We identified 5 novel de novo missense mutations in CALM2 in 3 subjects with LQTS (p.N98S, p.N98I, p.D134H) and 2 subjects with clinical features of both LQTS and CPVT (p.D132E, p.Q136P). Age of onset of major symptoms (syncope or cardiac arrest) ranged from 1 to 9 years. Three of 5 probands had cardiac arrest and 1 of these subjects did not survive. The clinical severity among subjects in this series was generally less than that originally reported for CALM1 and CALM2 associated with recurrent cardiac arrest during infancy. Four of 5 probands responded to β-blocker therapy, whereas 1 subject with mutation p.Q136P died suddenly during exertion despite this treatment. Mutations affect conserved residues located within Ca(2+)-binding loops III (p.N98S, p.N98I) or IV (p.D132E, p.D134H, p.Q136P) and caused reduced Ca(2+)-binding affinity. CONCLUSIONS: CALM2 mutations can be associated with LQTS and with overlapping features of LQTS and CPVT