Clinical effects of low-molecular-weight heparin combined with ulinastatin in children with acute pancreatitis

Purpose: To explore the clinical effects of low-molecular-weight heparin (LMWH) combined with ulinastatin (UTI) in children with acute pancreatitis.Methods: In total, 560 patients with severe acute pancreatitis treated at Binzhou People’s Hospital, Shandong, China, from April 2012 to June 2014 were enrolled in this study. They were divided into control (280 patients, ulinastatin + conventional treatment) and observational groups (280 patients, LMWH + ulinastatin + conventional treatment). The treatment lasted for 2 weeks. Clinical parameters, laboratory test indices, Acute Physiology and Chronic Health Evaluation (APACHE II) score, and computed tomography score of pancreatic necrosis (CTSPN) were assessed in both groups.Results: On admission, no significant differences were noted in clinical features, laboratory parameters, APACHE II scores, or CTSPN between the two groups (all p > 0.05). After 2 weeks of treatment, serum amylase, urine amylase, prothrombin time, fibrinogen, partial thromboplastin time, and platelet count in the study group were 913 ± 281 U/L, 1893 ± 295 U/L, 16 ± 1.60 s, 3 ± 0.60 g/L, 39.80 ± 5.60 s, and 294 ± 49 × 109/L, respectively, all of which were similar or superior to those in the control group (1738 ± 346 U/L, 2453 ± 473 U/L, 15 ± 1.50 S, 2.50 ± 0.50, 39.80 ± 5.90, and 192 ± 37 × 109/L)). APACHE II scores and CTSPN after 2 weeks of treatment in the observation group were 8.50 ± 1.80 and 2.10 ± 1, respectively, which were superior to those in the control group (9.60 ± 2.40 and 4.30 ± 2.60, respectively; p < 0.05). Moreover, the incidence of complications, mortality rate, and average duration of the hospital stay in the observation group were lower than those in the control group (p > 0.05). The cure rate in the observation group was higher than that in the control group.Conclusions: LMWH combined with UTI enhances the efficacy of conventional treatment and reduces mortality. Thus, it is a potentially effective treatment strategy for severe acute pancreatitis in children.Keywords: Acute pancreatitis, Low-molecular-weight heparin, Multiple organ function syndrome, APACHE II score, Pancreatic necrosi

N′-[(E)-2-Hydroxy­benzyl­idene]-5-methyl­isoxazole-4-carbohydrazide monohydrate

In the structure of the title compound, C12H11N3O3·H2O, the dihedral angle formed by the benzene and isoxazole rings is 2.03 (8)°. The mol­ecular conformation is stabilized by an intra­molecular O—H⋯N hydrogen bond. In the crystal structure, mol­ecules are linked into a three-dimesional network by inter­molecular N—H⋯O, O—H⋯N and O—H⋯O hydrogen bonds, and by π–π stacking inter­actions involving adjacent benzene and isoxazole rings [centroid–centroid separation = 3.663 (2) Å]

Optimization of Preparation Process of Chitosan-carrageenan Edible Composite Membrane Based on Response Surface Methodology

Chitosan was mixed with carrageenan and tea polyphenols, and chitosan-carrageenan composite film was prepared by the fluidized bed method and its properties were tested.The light transmittance,mechanical property and water resistance of the composite film were investigated by single factor test, and then the response surface experiment was carried out to optimize the preparation process of the composite film with water vapor transmittance as the evaluation index.The results showed that the optimal process of chitosan - carrageenan edible composite film was as follows: adding 1.5% of chitosan, 0.4% of carrageenan,0.6% of glycerol, 0.6% of tea polyphenols, and drying temperature of 50 ℃.Under this condition, the thickness of the composite film was uniform and the mechanical properties were good.The water vapor transmission rate was 1.37 (10–11 g/m·s·Pa)

A 3D Co(II) compound: Crystal structure and protective activity on chronic nephritis

37-42In the current study, via applying a mixed-ligand strategy, a Co(II) coordination polymer that is, {[Co4(3-dpyb)2 (odpa)2(H2O)3]ꞏ4H2O}n (1) [where 3-dpyb is N,N′-bis(3-pyridinecarboxamide)-1,4-butane, and H4odpa represents 4,4′-oxydiphthalic acid] is produced through reaction between two organic ligands and Co(NO3)2ꞏ6H2O in the water and DMF mixed solvents. For the treatment of chronic nephritis, real time RT-PCR is employed for measuring the AMPK signaling pathway activation, and ELISA detection kit is applied to evaluate inflammatory cytokines content released into plasma. Computer simulation has revealed that the activity of the proposed candidate is only from the carboxyl function groups, however, nitrogen atoms from both amide and pyridine groups only showed limited activity to the protein

RRM1 single nucleotide polymorphism -37C→A correlates with progression-free survival in NSCLC patients after gemcitabine-based chemotherapy

<p>Abstract</p> <p>Background</p> <p>The ribonucleotide reductase M1 (RRM1) gene encodes the regulatory subunit of ribonucleotide reductase, the molecular target of gemcitabine. The overexpression of RRM1 mRNA in tumor tissues is reported to be associated with gemcitabine resistance. Thus, single nucleotide polymorphisms (SNPs) of the RRM1 gene are potential biomarkers of the response to gemcitabine chemotherapy. We investigated whether RRM1 expression in peripheral blood mononuclear cells (PBMCs) or SNPs were associated with clinical outcome after gemcitabine-based chemotherapy in advanced non-small cell lung cancer (NSCLC) patients.</p> <p>Methods</p> <p>PBMC samples were obtained from 62 stage IIIB and IV patients treated with gemcitabine-based chemotherapy. RRM1 mRNA expression levels were assessed by real-time PCR. Three RRM1 SNPs, -37C→A, 2455A→G and 2464G→A, were assessed by direct sequencing.</p> <p>Results</p> <p>RRM1 expression was detectable in 57 PBMC samples, and SNPs were sequenced in 56 samples. The overall response rate to gemcitabine was 18%; there was no significant association between RRM1 mRNA expression and response rate (<it>P </it>= 0.560). The median progression-free survival (PFS) was 23.3 weeks in the lower expression group and 26.9 weeks in the higher expression group (<it>P </it>= 0.659). For the -37C→A polymorphism, the median PFS was 30.7 weeks in the C(-)37A group, 24.7 weeks in the A(-)37A group, and 23.3 weeks in the C(-)37C group (<it>P </it>= 0.043). No significant difference in PFS was observed for the SNP 2455A→G or 2464G→A.</p> <p>Conclusions</p> <p>The RRM1 polymorphism -37C→A correlated with PFS in NSCLC patients treated with gemcitabine-based chemotherapy. No significant correlation was found between PBMC RRM1 mRNA expression and the efficacy of gemcitabine.</p

Cancer survival: left truncation and comparison of results from hospital-based cancer registry and population-based cancer registry

BackgroundCancer survival is an important indicator for evaluating cancer prognosis and cancer care outcomes. The incidence dates used in calculating survival differ between population-based registries and hospital-based registries. Studies examining the effects of the left truncation of incidence dates and delayed reporting on survival estimates are scarce in real-world applications.MethodsCancer cases hospitalized at Nantong Tumor Hospital during the years 2002–2017 were traced with their records registered in the Qidong Cancer Registry. Survival was calculated using the life table method for cancer patients with the first visit dates recorded in the hospital-based cancer registry (HBR) as the diagnosis date (OSH), those with the registered dates of population-based cancer (PBR) registered as the incidence date (OSP), and those with corrected dates when the delayed report dates were calibrated (OSC).ResultsAmong 2,636 cases, 1,307 had incidence dates registered in PBR prior to the diagnosis dates of the first hospitalization registered in HBR, while 667 cases with incidence dates registered in PBR were later than the diagnosis dates registered in HBR. The 5-year OSH, OSP, and OSC were 36.1%, 37.4%, and 39.0%, respectively. The “lost” proportion of 5-year survival due to the left truncation for HBR data was estimated to be between 3.5% and 7.4%, and the “delayed-report” proportion of 5-year survival for PBR data was found to be 4.1%.ConclusionLeft truncation of survival in HBR cases was demonstrated. The pseudo-left truncation in PBR should be reduced by controlling delayed reporting and maximizing completeness. Our study provides practical references and suggestions for evaluating the survival of cancer patients with HBR and PBR

The dependent correlation between soil multifunctionality and bacterial community across different farmland soils

IntroductionMicroorganisms play a critical role in soil biogeochemical cycles, but it is still debated whether they influence soil biogeochemical processes through community composition and diversity or not. This study aims to investigate variation in bacterial community structure across different soils and its correlation to soil multifunctionality. Soil samples were collected from five typical farmland zones along distinct climatic gradients in China.MethodsThe high-throughput sequencing (Illumina MiSeq) of 16S rRNA genes was employed to analyze bacterial community composition in each soil sample. Multivariate analysis was used to determine the difference in soil properties, microbial community and functioning, and their interactions.ResultsCluster and discrimination analysis indicated that bacterial community composition was similar in five tested soil samples, but bacterial richness combined with soil enzyme activities and potential nitrification rate (PNR) contributed most to the differentiations of soil samples. Mantel test analysis revealed that bacterial community composition and richness were more significantly shaped by soil nutrient conditions and edaphic variables than bacterial diversity. As for soil multifunctionality, soil microbial community level physiological profiles were little affected by abiotic and biotic factors, while soil enzymes and PNR were also significantly related to bacterial community composition and richness, in addition to soil N and P availability.ConclusionCumulatively, soil enzymes’ activities and PNR were greatly dependent on bacterial community composition and richness not diversity, which in turn were greatly modified by soil N and P availability. Therefore, in the future it should be considered for the role of fertilization in the modification of bacterial community and the consequent control of nutrient cycling in soil

An Updated Search of Steady TeV γ−\gamma-Ray Point Sources in Northern Hemisphere Using the Tibet Air Shower Array

Using the data taken from Tibet II High Density (HD) Array (1997 February-1999 September) and Tibet-III array (1999 November-2005 November), our previous northern sky survey for TeV $\gamma-$ray point sources has now been updated by a factor of 2.8 improved statistics. From $0.0^{\circ}$ to $60.0^{\circ}$ in declination (Dec) range, no new TeV $\gamma-$ray point sources with sufficiently high significance were identified while the well-known Crab Nebula and Mrk421 remain to be the brightest TeV $\gamma-$ray sources within the field of view of the Tibet air shower array. Based on the currently available data and at the 90% confidence level (C.L.), the flux upper limits for different power law index assumption are re-derived, which are approximately improved by 1.7 times as compared with our previous reported limits.Comment: This paper has been accepted by hepn